Black Fly Rearing and Use in Laboratory Bioassays

2014 ◽  
pp. 42-72 ◽  
Author(s):  
Elmer Gray ◽  
Ray Noblet
Keyword(s):  
Black Fly ◽  
Laboratory Bioassays

In Vitro Screening of Azalea for Resistance to Azalea Lace Bug

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 506b-506
Author(s):  
Carol D. Robacker ◽  
S.K. Braman
Keyword(s):  
Leaf Damage ◽  
In Vitro Screening ◽  
Screening Assay ◽  
Delaware Valley ◽  
In Vitro Techniques ◽  
Culture Tube ◽  
Lace Bug ◽  
Lace Bugs ◽  
Laboratory Bioassays

Azalea lace bug (Stephanitis pyrioides) is the most serious pest on azalea. Results of laboratory bioassays and field evaluations of 17 deciduous azalea taxa have identified three resistant taxa: R. canescens, R. periclymenoides, and R. prunifolium. Highly susceptible taxa are `Buttercup', `My Mary', R. oblongifolium, and the evergreen cultivar `Delaware Valley White'. To determine whether in vitro techniques would have potential value in screening or selecting for resistance, or for the identification of morphological or chemical factors related to resistance, an in-vitro screening assay was developed. In-vitro shoot proliferation was obtained using the medium and procedures of Economou and Read (1984). Shoots used in the bioassays were grown in culture tubes. Two assays were developed: one for nymphs and one for adult lace bugs. To assay for resistance to nymphs, `Delaware Valley White' leaves containing lace bug eggs were disinfested with 70% alcohol and 20% commercial bleach, and incubated in sterile petri plates with moistened filter paper until the nymphs hatched. Five nymphs were placed in each culture tube, and cultures were incubated for about 2 weeks, or until adults were observed. To assay for resistance to adults, five female lace bugs were placed in each culture tube and allowed to feed for 5 days. Data collected on survival and leaf damage was generally supportive of laboratory bioassays and field results. Adult lace bugs had a low rate of survival on resistant taxa. Survival of nymphs was somewhat reduced on resistant taxa.

Larval black fly (Diptera: Simuliidae) distribution and diversity along a floodplain river in the Alps (Tagliamento River, Italy)

2002 ◽  
Vol 28 (2) ◽  
pp. 524-531
Author(s):  
David B. Arscott ◽  
Rolf Glatthaar ◽  
Klement Tockner ◽  
J. V. Ward
Keyword(s):  
Black Fly ◽  
Floodplain River ◽  
The Alps ◽  
Tagliamento River

Susceptibility of black fly larvae (Diptera: Simuliidae) to lawn-care insecticides individually and as mixtures

2003 ◽  
Vol 22 (7) ◽  
pp. 1582-1588 ◽  
Author(s):  
Jay P. Overmyer ◽  
Kevin L. Armbrust ◽  
Ray Noblet
Keyword(s):  
Black Fly ◽  
Lawn Care ◽  
Fly Larvae

scholarly journals Orientation of Onchocerca lienalis Stiles (Filarioidea: Onchocercidae) Microfilariae to Black Fly Saliva

2002 ◽  
Vol 39 (6) ◽  
pp. 908-914 ◽  
Author(s):  
T. Stallings ◽  
M. S. Cupp ◽  
E. W. Cupp
Keyword(s):  
Black Fly
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