Pulmonary Primary Cells Cocultured in a Novel Cell Culture System, the Integrated Discrete Multiple Cell–Type Coculture System (IdMOC): Pulmonary Cytotoxicity of Eight Cigarette Smoke Condensates and Nicotine

2014 ◽  
pp. 348-359 ◽  
Keyword(s):  
Cell Culture ◽  
Cigarette Smoke ◽  
Culture System ◽  
Primary Cells ◽  
Cell Culture System ◽  
Cell Type ◽  
Coculture System ◽  
Multiple Cell ◽  
Multiple Cell Type

In vitro assessment of food-derived-glucose bioaccessibility and bioavailability in bicameral cell culture system

2020 ◽  
Vol 45 (5) ◽  
pp. 631-637
Author(s):  
Cansu Ozel-Tasci ◽  
Gozde Pilatin ◽  
Ozgur Edeer ◽  
Sukru Gulec
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Metabolic Diseases ◽  
Enzymatic Digestion ◽  
Cell Culture System ◽  
Culture Studies ◽  
Experimental Approaches ◽  
In Vitro Assessment

AbstractBackgroundFunctional foods can help prevent metabolic diseases, and it is essential to evaluate functional characteristics of foods through in vitro and in vivo experimental approaches.ObjectiveWe aimed to use the bicameral cell culture system combined with the in vitro digestion to evaluate glucose bioavailability.Materials and methodsCake, almond paste, and pudding were modified by adding fiber and replacing sugar with sweeteners and polyols. Digestion process was modeled in test tubes. Rat enterocyte cells (IEC-6) were grown in a bicameral cell culture system to mimic the physiological characteristics of the human intestine. The glucose bioaccessibility and cellular glucose efflux were measured by glucose oxidase assay.Results and discussionThe glucose bioaccessibilities of modified foods were significantly lower (cake: 2.6 fold, almond paste: 9.2 fold, pudding 2.8 fold) than the controls. Cellular glucose effluxes also decreased in the modified cake, almond paste, and pudding by 2.2, 4, and 2 fold respectively compared to their controls.ConclusionOur results suggest that combining in vitro enzymatic digestion with cell culture studies can be a practical way to test in vitro glucose bioaccessibility and bioavailability in functional food development.

Multilayer cell culture system supported by thread

2018 ◽  
Vol 257 ◽  
pp. 650-657 ◽  
Author(s):  
Azadeh Nilghaz ◽  
Siew Hoo ◽  
Wei Shen ◽  
Xiaonan Lu ◽  
Peggy P.Y. Chan
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Cell Culture System

Environmental monitoring of urban streams using a primary fish gill cell culture system (FIGCS)

2015 ◽  
Vol 120 ◽  
pp. 279-285 ◽  
Author(s):  
Sabine Schnell ◽  
Kafilat Bawa-Allah ◽  
Adebayo Otitoloju ◽  
Christer Hogstrand ◽  
Thomas H. Miller ◽  
...  
Keyword(s):  
Cell Culture ◽  
Environmental Monitoring ◽  
Culture System ◽  
Urban Streams ◽  
Cell Culture System ◽  
Fish Gill ◽  
Gill Cell

Ho:YAG laser: intervertebral disk cell interaction using three-dimensional cell culture system

2000 ◽  
Author(s):  
Masato Sato ◽  
Miya Ishihara ◽  
Tsunenori Arai ◽  
Takashi Asazuma ◽  
Toshiyuki Kikuchi ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Three Dimensional ◽  
Cell Interaction ◽  
Intervertebral Disk ◽  
Cell Culture System ◽  
Ho:Yag Laser ◽  
Dimensional Cell

A Cell Culture System for Study of the Development of Toxoplasma gondii Bradyzoites

1995 ◽  
Vol 42 (2) ◽  
pp. 150-157 ◽  
Author(s):  
LOUIS M. WEISS ◽  
DENISE LAPLACE ◽  
PETER M. TAKVORIAN ◽  
HERBERT B. TANOWITZ ◽  
ANN CALI ◽  
...  
Keyword(s):  
Cell Culture ◽  
Toxoplasma Gondii ◽  
Culture System ◽  
Cell Culture System ◽  
A Cell

scholarly journals Interferon treatment suppresses enteric adenovirus infection in a model gastrointestinal cell-culture system

2012 ◽  
Vol 93 (3) ◽  
pp. 618-623 ◽  
Author(s):  
Victoria Sherwood ◽  
Elizabeth King ◽  
Sabine Tötemeyer ◽  
Ian Connerton ◽  
Kenneth H. Mellits
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Adenovirus Type ◽  
Host Cells ◽  
Adenovirus Infection ◽  
Cell Culture System ◽  
Cell Culture Model ◽  
Culture Model ◽  
Enteric Adenovirus ◽  
Transcriptional Induction

Exposure to interferon results in the rapid transcriptional induction of genes, many of which function to create an antiviral environment in potential host cells. For the majority of adenoviruses, replication is unaffected by the actions of interferon. It has previously been shown, using non-gastrointestinal cells, that the species F human adenoviruses are sensitive to the action of interferon. Here, we have developed an enterocyte-like cell-culture model to re-evaluate this question, and determined the effects of interferon on species F adenovirus during infection of gastrointestinal cells. We show that species F adenovirus type 40 is sensitive to the effects of interferon in gastrointestinal-like cells, which may help to explain its fastidious growth in culture.

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