Living Skin Equivalents and Skin Organ Culture: Preclinical Models for Cosmetic Efcacy Testing

2014 ◽  
pp. 315-326
Keyword(s):  
Organ Culture ◽  
Preclinical Models ◽  
Skin Equivalents ◽  
Skin Organ Culture ◽  
Living Skin Equivalents

Colonization of living skin equivalents by Malassezia furfur

1998 ◽  
Vol 36 (1) ◽  
pp. 15-19
Author(s):  
BHATTACHARYYA ◽  
EDWARD ◽  
CORDERY ◽  
RICHARDSON
Keyword(s):  
Malassezia Furfur ◽  
Skin Equivalents ◽  
Living Skin Equivalents

The Fixation of Living Skin Equivalents

2006 ◽  
Vol 14 (1) ◽  
pp. 122-125 ◽  
Author(s):  
Hye-Ryung Choi ◽  
Soon-Kyum Kim ◽  
Sun-Bang Kwon ◽  
Kyoung-Chan Park
Keyword(s):  
Skin Equivalents ◽  
Living Skin Equivalents

scholarly journals Kinetic Cytokine Secretion Profile of LPS-Induced Inflammation in the Human Skin Organ Culture

Pharmaceutics ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 299 ◽  
Author(s):  
Raanan Gvirtz ◽  
Navit Ogen-Shtern ◽  
Guy Cohen
Keyword(s):  
Organ Culture ◽  
Human Skin ◽  
Comprehensive Evaluation ◽  
Ex Vivo ◽  
Cytokine Secretion ◽  
Peak Time ◽  
Local Skin ◽  
The Impact ◽  
Skin Organ Culture

Several in vitro models that mimic different aspects of local skin inflammation exist. The use of ex vivo human skin organ culture (HSOC) has been reported previously. However, comprehensive evaluation of the cytokine secretory capacity of the system and its kinetics has not been performed. Objective: the aim of the current study was to investigate the levels and secretion pattern of key cytokine from human skin tissue upon lipopolysaccharide (LPS) stimulation. HSOC maintained in an air–liquid interface was used. Epidermal and tissue viability was monitored by MTT and Lactate Dehydrogenase (LDH) activity assay, respectively. Cytokine levels were examined by ELISA and multiplex array. HSOCs were treated without or with three different LPS subtypes and the impact on IL-6 and IL-8 secretion was evaluated. The compounds enhanced the secreted levels of both cytokines. However, differences were observed in their efficacy and potency. Next, a kinetic multiplex analysis was performed on LPS-stimulated explants taken from three different donors to evaluate the cytokine secretion pattern during 0–72 h post-induction. The results revealed that the pro-inflammatory cytokines IL-6, IL-8, TNFα and IL-1β were up-regulated by LPS stimuli. IL-10, an anti-inflammatory cytokine, was also induced by LPS, but exhibited a different secretion pattern, peak time and maximal stimulation values. IL-1α and IL-15 showed donor-specific changes. Lastly, dexamethasone attenuated cytokine secretion in five independent repetitions, supporting the ability of the system to be used for drug screening. The collective results demonstrate that several cytokines can be used as valid inflammatory markers, regardless of changes in the secretion levels due to donor’s specific alterations.

scholarly journals Development of a Short-Term Canine Full-Thickness Skin Organ Culture Method under Serum-Free Conditions

2016 ◽  
Vol 11 (2) ◽  
pp. 61-69 ◽  
Author(s):  
Francesca Abramo ◽  
Andrea Pirone ◽  
Carla Lenzi ◽  
Maria Federica della Valle ◽  
Silvia Vidali ◽  
...  
Keyword(s):  
Organ Culture ◽  
Culture Method ◽  
Full Thickness ◽  
Short Term ◽  
Serum Free ◽  
Full Thickness Skin ◽  
Thickness Skin ◽  
Serum Free Conditions ◽  
Skin Organ Culture
Sign in / Sign up

Export Citation Format

Share Document