COX-2 Gene Expression in Colon Cancer Tissue Related to Regulating Factors and Promoter Methylation Status

2014 ◽  
pp. 29-50
Author(s):  
Annika Asting ◽  
Helena Carén ◽  
Marianne Andersson ◽  
Christina Lönnroth ◽  
Kristina Lagerstedt ◽  
...  
Keyword(s):  
Gene Expression ◽  
Colon Cancer ◽  
Promoter Methylation ◽  
Methylation Status ◽  
Cancer Tissue ◽  
Colon Cancer Tissue ◽  
Promoter Methylation Status ◽  
Cox 2

scholarly journals COX-2 gene expression in colon cancer tissue related to regulating factors and promoter methylation status

BMC Cancer ◽  
2011 ◽  
Vol 11 (1) ◽  
Author(s):  
Annika Gustafsson Asting ◽  
Helena Carén ◽  
Marianne Andersson ◽  
Christina Lönnroth ◽  
Kristina Lagerstedt ◽  
...  
Keyword(s):  
Gene Expression ◽  
Colon Cancer ◽  
Promoter Methylation ◽  
Methylation Status ◽  
Cancer Tissue ◽  
Colon Cancer Tissue ◽  
Promoter Methylation Status ◽  
Cox 2

Gene-Specific Methylation: Potential Markers for Colorectal Cancer

2009 ◽  
Vol 24 (1) ◽  
pp. 57-62 ◽  
Author(s):  
Jyothi S. Prabhu ◽  
Aruna Korlimarla ◽  
Arindam Banerjee ◽  
Shivangi Wani ◽  
K Payal ◽  
...  
Keyword(s):  
Gene Expression ◽  
Colorectal Cancer ◽  
Polymerase Chain Reaction ◽  
Promoter Methylation ◽  
Methylation Status ◽  
Epigenetic Mechanism ◽  
Aberrant Methylation ◽  
Chain Reaction ◽  
Promoter Methylation Status ◽  
Polymerase Chain

Purpose Aberrant methylation of the promoter region is associated with silencing of many genes in neoplasia. CpG island methylation is an epigenetic mechanism for transcriptional silencing that occurs at various stages of colon tumorigenesis. In this study, we tested the promoter methylation and expression of seven genes from various pathways of DNA repair, apoptosis and inflammation, i.e., sFRP1, MLH1, RASSF1A, CDA, v-fgr, LYN-B, and TNFR10d. Method The genes were analyzed by quantitative polymerase chain reaction for the level of gene expression. The promoter methylation status of the genes was studied by methylation-specific polymerase chain reaction. Result The correlation of promoter methylation status with suppressed gene expression patterns suggested a potential role for the silencing these genes in colon cancer progression. Conclusion Promoter methylations of the studied genes could be explored as promising biomarkers for new diagnostic, prognostic and therapeutic targets of colorectal cancer.

Prediction Biomarkers Associated with Lymph Node Metastasis and Prognosis were Identified in Papillary Thyroid Carcinoma via Integrated Bioinformatics Analysis

2020 ◽  
Vol 23 ◽  
Author(s):  
Siyuan Gan ◽  
Ruifang Ye ◽  
Yanping Ha ◽  
Yinghuan Xiong ◽  
Rujia Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Lymph Node ◽  
Thyroid Carcinoma ◽  
Promoter Methylation ◽  
Methylation Status ◽  
Papillary Thyroid ◽  
Ppi Network ◽  
Promoter Methylation Status ◽  
Node Metastasis ◽  
Core Genes

Background:: Prediction biomarkers associated with prognosis and lymph node metastasis (LNM) in papillary thyroid carcinoma (PTC) are needed to facilitate clinicians in choosing appropriate therapies. Objective:: We hope to identify key genes associated with LNM and prognosis in PTC. Methods:: GSE29265, GSE33630, GSE3467, GSE3678 and GSE58545 gene expression profiles were acquired from the Gene Expression Omnibus database. Differentially expressed genes (DEGs) between PTC tissues and normal thyroid tissues were selected with the GEO2R tool, and common DEGs among the five datasets were integrated with Venn software online. A protein-protein interaction (PPI) network of the common DEGs was visualized. We analyzed the PPI network and determined core genes using the Cytoscape software. Furthermore, we employed UALCAN to verify the expression and promoter methylation status of the core genes in thyroid carcinoma (THCA). Additionally, the Kaplan–Meier plotter online tool was used to analyze the relationship between overall survival and core gene expressions in THCA. Results:: TNS3, DUSP6, DUSP4 and PTPRE were identified as core genes. Expression of these 4 genes and the promoter methylation status of DUSP4 and PTPRE were strongly associated with LNM (P<0.05). High expression of 3 genes (DUSP6, DUSP4 and PTPRE) was related with significantly better survival than low expression of the 3 genes in THCA. In contrast, high TNS3 expression was related to significantly worse survival (P<0.05). Conclusion:: TNS3, DUSP6, DUSP4, PTPRE and DUSP4 and PTPRE promoter methylation status might be useful predictive biomarkers of LNM in PTC. Additionally, these genes may be prognostic biomarkers in PTC.

Could CYP24A1 promoter methylation status affect the gene expression in the colorectal cancer patients?

Meta Gene ◽  
2020 ◽  
Vol 24 ◽  
pp. 100656
Author(s):  
Hossein Sadeghi ◽  
Ehsan Nazemalhosseini-Mojarad ◽  
Vahid Reza Yassaee ◽  
Sanaz Savabkar ◽  
Majid Ghasemian ◽  
...  
Keyword(s):  
Gene Expression ◽  
Colorectal Cancer ◽  
Cancer Patients ◽  
Promoter Methylation ◽  
Methylation Status ◽  
Promoter Methylation Status ◽  
Colorectal Cancer Patients
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