Vitamin Analysis in Food by UPLC–MS

2014 ◽  
pp. 243-278
Author(s):  
Ahmad Aqel ◽  
Kareem Yusuf ◽  
Asma’a Al-Rifai ◽  
Zeid Alothman
Keyword(s):  
Vitamin Analysis

scholarly journals Vitamin Analysis, Trace Elements Content, and Their Extractabilities in Tetrapleura tetraptera

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Prince Oteng ◽  
John K. Otchere ◽  
Stephen Adusei ◽  
Richard Q. Mensah ◽  
Emmanuel Tei-Mensah
Keyword(s):  
Trace Elements ◽  
Vitamin A ◽  
Vitamin C ◽  
Trace Element ◽  
Trace Element Content ◽  
Beta Carotene ◽  
Flame Atomic Absorption ◽  
Significant Difference ◽  
Tetrapleura Tetraptera ◽  
Vitamin Analysis

Tetrapleura tetraptera is widely cherished in African traditional homes because of its alleged therapeutic and nutritional properties. This present study aimed at determining the levels of vitamin A, C, E, and beta-carotene and trace element (Fe, Cu, Mn, Co, Se, and Zn) concentrations and their extractabilities in the pulp, seeds, and whole fruit (mixture of pulp and seeds) of T. tetraptera. The total trace element concentration of Fe, Cu, Co, Mn, and Zn and their extractabilities (%) were determined using flame atomic absorption spectrometer (FAAS), whereas UV-VIS spectrophotometer was used to determine selenium concentration. The trace element content (mg/kg) based on dry weight in the pulp, seeds, and whole fruit was Fe (162.00 ± 7.14, 115.00 ± 12.00, and 154.00 ± 25.20, respectively), Zn (31.60 ± 4.77, 43.40 ± 5.29, and 41.50 ± 8.97, respectively), Cu (16.10 ± 4.98, 11.90 ± 8.40, and 17.20 ± 14.50, respectively), Mn (55.30 ± 2.41, 156.00 ± 10.20, and 122.00 ± 5.29, respectively), Co (38.10 ± 6.40, 21.10 ± 7.15, and 44.00 ± 14.90, respectively), and Se (1.49 ± 0.17, 2.43 ± 0.28, and 2.97 ± 0.27 μg/g, respectively). The mineral extractabilities (%) in the pulp, seeds, and whole fruit of T. tetraptera were established to be in the order Co > Zn > Fe > Cu > Se > Mn. Also, the chromatographic method (HPLC) was used to evaluate vitamin E concentration, and vitamin C and concentration of beta-carotene were calculated from the obtained concentration of vitamin A using a conversion factor by the titrimetric method. From the results of vitamin analysis, a significant difference (p<0.05) was observed among the pulp, seeds, and whole fruit for vitamin C and E. However, no significant difference (p>0.05) was perceived among these plant parts for vitamin A and beta-carotene. This study has therefore revealed that the pulp, seeds, and whole fruit of T. tetraptera contain varying concentrations of vitamins and trace elements and has given many vital insights on which part of T. tetraptera to consume, as concentrations of these nutrients differ in the discrete parts of the fruit.

Some applications of fluorimetry in vitamin analysis

The Analyst ◽  
1947 ◽  
Vol 72 (858) ◽  
pp. 385 ◽  
Author(s):  
E. Kodicek
Keyword(s):  
Vitamin Analysis

scholarly journals Nutrient Analysis and Antimicrobial Activities of the Leaves and Fruit Pulp Extracts of Tetrapluera tetraptera on Clinical Bacteria Isolates

2020 ◽  
pp. 21-31
Author(s):  
R. U. B. Ebana ◽  
U. O. Edet ◽  
I. E. Andy ◽  
C. A. Etok ◽  
V. J. Etim ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Activities ◽  
Fruit Pulp ◽  
Nutrient Analysis ◽  
Robust Analysis ◽  
Zn And Cu In ◽  
Permissible Levels ◽  
Potential Antimicrobial Activity ◽  
Vitamin Analysis ◽  
Standard Disc

Introduction: Plants are known to contain numerous phytochemicals with potential antimicrobial activity. Aim: Nutritional, phytochemical and antimicrobial activities of the leaves and the fruit pulp extracts of Tetrapluera tetraptera was carried out. Methodology: Collection and identification of isolates and plants parts, and preparation of extracts and nutrient (proximate, vitamins and minerals and anti-nutrients) evaluations were all done using standard protocols previously reported. Evaluations of phytochemicals were carried out using crude screening, quantification and gas chromatography coupled to mass spectrophotometer for a              robust analysis. Antimicrobial activities were evaluated using the standard disc method and chloramphenicol as control. Results: The results revealed varying amounts of proximate nutrients (ash, fibre, moisture, carbohydrate, protein and fat) in both samples. Vitamin analysis showed the presence of vitamin A, total and soluble vitamin C, niacin, riboflavin and thiamin in both samples. Furthermore, both samples had minerals such as Na, K, CA, P, Mg, Zn, Fe, and Cu, and K, Na, Ca, Mg, P, Fe, Zn and Cu in order of decreasing abundance in the fruit pulp and leaves, respectively. Both samples showed the presence of permissible levels of anti-nutrients compared to edible vegetables. Using all three methods, several phytochemicals such as terpenes, alkaloids, flavonoids, polyphenol, organic and fatty acids, amongst other in various amounts were obtained in the samples. Antimicrobial activities against identified clinical isolates used in this study which were Escherichia coli, Staphylococcus aureus and Proteus spp with both extracts ranged from 12.78±0.03 to 17.34±0.34 mm. Conclusion: Given the rising cases of antimicrobial resistance and absence of newer antibiotics, the antimicrobial activity of our study plant is worth further evaluations.

Vitamin Analysis in Infant Formulas

1982 ◽  
Vol 65 (6) ◽  
pp. 1491-1494
Author(s):  
Alvin W Granade
Keyword(s):  
Infant Formula ◽  
Drug Administration ◽  
Food And Drug Administration ◽  
Infant Formulas ◽  
Regional Laboratory ◽  
Vitamin Analysis

Abstract The methodology used by the Atlanta Regional Laboratory, U.S. Food and Drug Administration, for determination of vitamins in infant formulas is summarized. Analytical findings for 11 vitamins are presented by manufacturer in terms of the requirements of the Infant Formula Act and percentage of the manufacturer’s label declaration.

New Approaches in Vitamin Analysis: Objectives and Strategy

1988 ◽  
Vol 71 (2) ◽  
pp. 447-447
Author(s):  
Paul M Finglas ◽  
David A T Southgate ◽  
Michael R A Morgan
Keyword(s):  
New Approaches ◽  
Vitamin Analysis
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