Cellular Lipids and Inammation

2014 ◽  
pp. 51-64
Keyword(s):  
Cellular Lipids

scholarly journals Ferroptotic cell death triggered by conjugated linolenic acids is mediated by ACSL1

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Alexander Beatty ◽  
Tanu Singh ◽  
Yulia Y. Tyurina ◽  
Vladimir A. Tyurin ◽  
Svetlana Samovich ◽  
...  
Keyword(s):  
Cell Death ◽  
Therapeutic Potential ◽  
Neutral Lipids ◽  
Cell Types ◽  
Lipid Hydroperoxides ◽  
Conjugated Linolenic Acids ◽  
Cellular Lipids ◽  
Transcriptional Changes ◽  
Acyl Chains ◽  
Tumor Growth And Metastasis

AbstractFerroptosis is associated with lipid hydroperoxides generated by the oxidation of polyunsaturated acyl chains. Lipid hydroperoxides are reduced by glutathione peroxidase 4 (GPX4) and GPX4 inhibitors induce ferroptosis. However, the therapeutic potential of triggering ferroptosis in cancer cells with polyunsaturated fatty acids is unknown. Here, we identify conjugated linoleates including α-eleostearic acid (αESA) as ferroptosis inducers. αESA does not alter GPX4 activity but is incorporated into cellular lipids and promotes lipid peroxidation and cell death in diverse cancer cell types. αESA-triggered death is mediated by acyl-CoA synthetase long-chain isoform 1, which promotes αESA incorporation into neutral lipids including triacylglycerols. Interfering with triacylglycerol biosynthesis suppresses ferroptosis triggered by αESA but not by GPX4 inhibition. Oral administration of tung oil, naturally rich in αESA, to mice limits tumor growth and metastasis with transcriptional changes consistent with ferroptosis. Overall, these findings illuminate a potential approach to ferroptosis, complementary to GPX4 inhibition.

scholarly journals Changes in Cell Membrane and Cellular Lipids in Apoptotic Cells Induced by Dolichyl Phosphate Differ from Findings in Cells Induced by Etoposide.

2002 ◽  
Vol 51 (1) ◽  
pp. 35-42 ◽  
Author(s):  
Akiko HORIUCHI ◽  
Etsuko YASUGI ◽  
Chizu IWASAKI ◽  
Keiji FUJIMOTO ◽  
Mieko OSHIMA
Keyword(s):  
Cell Membrane ◽  
Apoptotic Cells ◽  
Dolichyl Phosphate ◽  
Cellular Lipids ◽  
In Cells

scholarly journals Simultaneous isolation of total cellular lipids and RNA from cultured cells

BioTechniques ◽  
2006 ◽  
Vol 41 (4) ◽  
pp. 426-430 ◽  
Author(s):  
David Akopian ◽  
Jheem D. Medh
Keyword(s):  
Cultured Cells ◽  
Cellular Lipids

Esterification of an endogenously synthesized lipoxygenase product into granulocyte cellular lipids

Biochemistry ◽  
1981 ◽  
Vol 20 (18) ◽  
pp. 5297-5301 ◽  
Author(s):  
Robert W. Bonser ◽  
Marvin I. Siegel ◽  
Sophia M. Chung ◽  
Randy T. McConnell ◽  
Pedro Cuatrecasas
Keyword(s):  
Lipoxygenase Product ◽  
Cellular Lipids

Cellular Lipids and Inflammation

2014 ◽  
pp. 39-52
Author(s):  
David Heber ◽  
Susanne Henning
Keyword(s):  
Cellular Lipids

scholarly journals Labeled vs. Label-Free Raman Imaging of Lipids in Endothelial Cells of Various Origins

Molecules ◽  
2020 ◽  
Vol 25 (23) ◽  
pp. 5752
Author(s):  
Basseem Radwan ◽  
Adriana Adamczyk ◽  
Szymon Tott ◽  
Krzysztof Czamara ◽  
Katarzyna Kaminska ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Endothelial Cells ◽  
Lipid Content ◽  
Single Layer ◽  
Vascular Diseases ◽  
Raman Imaging ◽  
Dependent Manner ◽  
Label Free ◽  
Necrosis Factor Alpha ◽  
Cellular Lipids

Endothelial cells (EC) constitute a single layer of the lining of blood vessels and play an important role in maintaining cardiovascular homeostasis. Endothelial dysfunction has been recognized as a primary or secondary cause of many diseases and it manifests itself, among others, by increased lipid content or a change in the lipid composition in the EC. Therefore, the analysis of cellular lipids is crucial to understand the mechanisms of disease development. Tumor necrosis factor alpha (TNF-α)-induced inflammation of EC alters the lipid content of cells, which can be detected by Raman spectroscopy. By default, lipid detection is carried out in a label-free manner, and these compounds are recognized based on their spectral profile characteristics. We consider (3S,3′S)-astaxanthin (AXT), a natural dye with a characteristic resonance spectrum, as a new Raman probe for the detection of lipids in the EC of various vascular beds, i.e., the aorta, brain and heart. AXT colocalizes with lipids in cells, enabling imaging of lipid-rich cellular components in a time-dependent manner using laser power 10 times lower than that commonly used to measure biological samples. The results show that AXT can be used to study lipids distribution in EC at various locations, suggesting its use as a universal probe for studying cellular lipids using Raman spectroscopy. The use of labeled Raman imaging of lipids in the EC of various organs could contribute to their easier identification and to a better understanding of the development and progression of various vascular diseases, and it could also potentially improve their diagnosis and treatment.

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