Anti-Oxidative Effects of the Biennial Flower of Panax Notoginseng Against H2O2-Induced Cytotoxicity in Cultured PC12 Cells

2014 ◽  
pp. 115-130
Keyword(s):  
Pc12 Cells ◽  
Panax Notoginseng ◽  
Oxidative Effects

scholarly journals Anti-oxidative effects of the biennial flower of Panax notoginseng against H2O2-induced cytotoxicity in cultured PC12 cells

2010 ◽  
Vol 5 (1) ◽  
pp. 38 ◽  
Author(s):  
Roy Choi ◽  
Zhiyong Jiang ◽  
Heidi Xie ◽  
Anna Cheung ◽  
David Lau ◽  
...  
Keyword(s):  
Pc12 Cells ◽  
Panax Notoginseng ◽  
Oxidative Effects

scholarly journals Erratum: Neuroprotective Effects of Dammarane-Type Saponins from Panax notoginseng on Glutamate-Induced Cell Damage in PC12 Cells

Planta Medica ◽  
2019 ◽  
Vol 85 (09/10) ◽  
pp. e2-e2
Author(s):  
Bao-Bao Zhang ◽  
Xiao-Long Hu ◽  
Yu-Yan Wang ◽  
Jun-Yan Li ◽  
Thi-Anh Pham ◽  
...  
Keyword(s):  
Pc12 Cells ◽  
Cell Damage ◽  
Panax Notoginseng ◽  
Neuroprotective Effects

scholarly journals Exploring the Mechanism of Panax notoginseng Saponins against Alzheimer’s Disease by Network Pharmacology and Experimental Validation

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Yixuan Jiang ◽  
Shanliang Li ◽  
Xiaoqin Xie ◽  
Hemei Li ◽  
Panling Huang ◽  
...  
Keyword(s):  
Pc12 Cells ◽  
Neuroprotective Effect ◽  
Panax Notoginseng ◽  
Network Pharmacology ◽  
Protective Effects ◽  
Panax Notoginseng Saponins ◽  
Active Ingredients ◽  
Target Disease ◽  
And Function

Background. Panax notoginseng saponins (PNS) have been used for neurodegenerative disorders such as cerebral ischemia and Alzheimer’s disease (AD). Although increasing evidences show the neuron protective effects of PNS, the vital compounds and their functional targets remain elusive. To explore the potential functional ingredients of PNS for the AD treatment and their molecular mechanisms, an in vitro neuron injured model induced by Aβ was investigated, and the potential mechanism was predicted by network pharmacology approach and validated by molecular biology methods. Methods. Network pharmacology approach was used to reveal the relationship between ingredient-target disease and function-pathway of PNS on the treatment of AD. The active ingredients of PNS were collected from TCMSP, PubChem database, and literature mining in PubMed database. DrugBank and GeneCards database were used to predict potential targets for AD. The STRING database was performed to reveal enrichment of these target proteins, protein-protein interactions, and related pathways. Networks were visualized by utilizing Cytoscape software. The enrichment analysis was performed by the DAVID database. Finally, neuroprotective effect and predictive mechanism of PNS were investigated in an in vitro AD model established by Aβ25–35-treated PC12 cells. Results. An ingredient-target disease and function-pathway network demonstrated that 38 active ingredients were derived from PNS modulated 364 common targets shared by PNS and AD. GO and KEGG analysis, further clustering analysis, showed that mTOR signaling targets were associated with the neuroprotective effects of PNS. In Aβ-treated PC12 cells, PNS treatment improved neuroprotective effect, including mTOR inhibition and autophagy activation. Conclusions. Collectively, the protective effects of PNS on AD-neuron injury are related to the inhibition of mTOR and autophagy activation.

Neuroprotective Effects of Dammarane-Type Saponins from Panax notoginseng on Glutamate-Induced Cell Damage in PC12 Cells

Planta Medica ◽  
2019 ◽  
Vol 85 (09/10) ◽  
pp. 692-700 ◽  
Author(s):  
Bao-Bao Zhang ◽  
Xiao-Long Hu ◽  
Yu-Yan Wang ◽  
Jun-Yan Li ◽  
Thi-Anh Pham ◽  
...  
Keyword(s):  
Neurodegenerative Diseases ◽  
Pc12 Cells ◽  
Cell Damage ◽  
Reactive Oxygen Species Generation ◽  
Panax Notoginseng ◽  
Cytochrome C Release ◽  
Neuroprotective Effects ◽  
Excitatory Neurotransmitter ◽  
Highly Active ◽  
Dependent Protein

AbstractDammarane-type saponins, the main active ingredients of Panax notoginseng, have substantial neuroprotective effects in different animal models of neurodegenerative diseases. However, because these compounds have different structures, the level of protection provided by individual compounds varies, and highly active compounds can be selected based on structure-activity relationships. Glutamate is a major excitatory neurotransmitter that plays an important role in synaptic response development. However, excessive extracellular glutamate levels lead to neuronal dysfunctions in the central nervous system. Herein, we investigated the neuroprotective effects of nine saponins (compounds 1 – 9) on glutamate-treated PC12 cells in the concentration range of 0.1 – 10 µM. The MTT assay revealed that these compounds increased cell viability to 65.6, 69.8, 76.9, 91.7, 74.4, 63.3, 59.9, 64.7, and 59.9%, respectively, compared with the glutamate-treated cells (44.6%). Protopanaxatriol (compound 4) was the most neuroprotective compound, and subsequent experiments revealed that pretreatment with compound 4 significantly reverses mitochondrial membrane potential collapse, increases superoxide dismutase activity, and decreases lactate dehydrogenase leakage, malondiadehyde levels, reactive oxygen species generation, and cell apoptosis. Compound 4 also decreased the Bax/Bcl-2 ratio, cleaved caspase-3, N-methyl-D-aspartic receptor 1, and Ca2+-/calmodulin-dependent protein kinase II expression, and inhibited glutamate-induced cytochrome C release and phosphorylation of apoptosis signal-regulating kinase 1, c-Jun N-terminal kinase, and p38. Overall, the results indicate that protopanaxatriol has significant neuroprotective effects, and might be a promising neuroprotective agent for preventing and treating neurodegenerative diseases.

Triterpenoids with Promoting Effects on the Differentiation of PC12 Cells from the Steamed Roots of Panax notoginseng

2015 ◽  
Vol 78 (8) ◽  
pp. 1829-1840 ◽  
Author(s):  
Cheng-Zhen Gu ◽  
Jun-Jiang Lv ◽  
Xiao-Xia Zhang ◽  
Yi-Jun Qiao ◽  
Hui Yan ◽  
...  
Keyword(s):  
Pc12 Cells ◽  
Panax Notoginseng
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