scholarly journals Modeling Robustness Tradeoffs in Yeast Cell Polarization Induced by Spatial Gradients

2014 ◽  
pp. 41-78
Author(s):  
Ching-Shan Chou ◽  
Qing Nie ◽  
Tau-Mu Yi
Keyword(s):  
Yeast Cell ◽  
Cell Polarization ◽  
Spatial Gradients

scholarly journals Modeling Robustness Tradeoffs in Yeast Cell Polarization Induced by Spatial Gradients

PLoS ONE ◽  
2008 ◽  
Vol 3 (9) ◽  
pp. e3103 ◽  
Author(s):  
Ching-Shan Chou ◽  
Qing Nie ◽  
Tau-Mu Yi
Keyword(s):  
Yeast Cell ◽  
Cell Polarization ◽  
Spatial Gradients

scholarly journals The GTP-binding Protein Rho1p Is Required for Cell Cycle Progression and Polarization of the Yeast Cell

1999 ◽  
Vol 146 (2) ◽  
pp. 373-387 ◽  
Author(s):  
Jana Drgonová ◽  
Tomás Drgon ◽  
Dong-Hyun Roh ◽  
Enrico Cabib
Keyword(s):  
Cell Cycle ◽  
Yeast Cell ◽  
Cell Cycle Progression ◽  
Binding Protein ◽  
Cell Polarization ◽  
Nonpermissive Temperature ◽  
Gtp Binding Protein ◽  
Cycle Progression ◽  
Glucan Synthase ◽  
Gtp Binding

Previous work showed that the GTP-binding protein Rho1p is required in the yeast, Saccharomyces cerevisiae, for activation of protein kinase C (Pkc1p) and for activity and regulation of β(1→3)glucan synthase. Here we demonstrate a hitherto unknown function of Rho1p required for cell cycle progression and cell polarization. Cells of mutant rho1E45I in the G1 stage of the cell cycle did not bud at 37°C. In those cells actin reorganization and recruitment to the presumptive budding site did not take place at the nonpermissive temperature. Two mutants in adjacent amino acids, rho1V43T and rho1F44Y, showed a similar behavior, although some budding and actin polarization occurred at the nonpermissive temperature. This was also the case for rho1E45I when placed in a different genetic background. Cdc42p and Spa2p, two proteins that normally also move to the bud site in a process independent from actin organization, failed to localize properly in rho1E45I. Nuclear division did not occur in the mutant at 37°C, although replication of DNA proceeded slowly. The rho1 mutants were also defective in the formation of mating projections and in congregation of actin at the projections in the presence of mating pheromone. The in vitro activity of β(1→3)glucan synthase in rho1 E45I, although diminished at 37°C, appeared sufficient for normal in vivo function and the budding defect was not suppressed by expression of a constitutively active allele of PKC1. Reciprocally, when Pkc1p function was eliminated by the use of a temperature-sensitive mutation and β(1→3)glucan synthesis abolished by an echinocandin-like inhibitor, a strain carrying a wild-type RHO1 allele was able to produce incipient buds. Taken together, these results reveal a novel function of Rho1p that must be executed in order for the yeast cell to polarize.

scholarly journals ROLE OF SMALL G PROTEINS IN YEAST CELL POLARIZATION AND WALL BIOSYNTHESIS

1998 ◽  
Vol 67 (1) ◽  
pp. 307-333 ◽  
Author(s):  
Enrico Cabib ◽  
Jana Drgonová ◽  
Tomás Drgon
Keyword(s):  
Yeast Cell ◽  
G Proteins ◽  
Cell Polarization ◽  
Small G Proteins

Modeling Yeast Cell Polarization Induced by Pheromone Gradients

2007 ◽  
Vol 128 (1-2) ◽  
pp. 193-207 ◽  
Author(s):  
Tau-Mu Yi ◽  
Shanqin Chen ◽  
Ching-Shan Chou ◽  
Qing Nie
Keyword(s):  
Yeast Cell ◽  
Cell Polarization

scholarly journals A Predictive Model for Yeast Cell Polarization in Pheromone Gradients

2016 ◽  
Vol 12 (4) ◽  
pp. e1004795 ◽  
Author(s):  
Nicolas Muller ◽  
Matthieu Piel ◽  
Vincent Calvez ◽  
Raphaël Voituriez ◽  
Joana Gonçalves-Sá ◽  
...  
Keyword(s):  
Yeast Cell ◽  
Predictive Model ◽  
Cell Polarization

scholarly journals The first buds of Cdc42

2015 ◽  
Vol 209 (6) ◽  
pp. 780-780 ◽  
Author(s):  
Ben Short
Keyword(s):  
Yeast Cell ◽  
Cell Polarization ◽  
Small Gtpase

In 1990, John Pringle and colleagues identified the small GTPase and demonstrated its role in yeast cell polarization.

scholarly journals Bem1 complexes and the complexity of yeast cell polarization

2009 ◽  
Vol 19 (5) ◽  
pp. R194-R195 ◽  
Author(s):  
Rong Li ◽  
Roland Wedlich-Soldner
Keyword(s):  
Yeast Cell ◽  
Cell Polarization
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