Improvement of Hydrogen Production through Molecular Approaches and Metabolic Engineering

2014 ◽  
pp. 199-238
Keyword(s):  
Metabolic Engineering ◽  
Hydrogen Production ◽  
Molecular Approaches

scholarly journals Metabolic engineering of Caldicellulosiruptor bescii yields increased hydrogen production from lignocellulosic biomass

2013 ◽  
Vol 6 (1) ◽  
pp. 85 ◽  
Author(s):  
Minseok Cha ◽  
Daehwan Chung ◽  
James G Elkins ◽  
Adam M Guss ◽  
Janet Westpheling
Keyword(s):  
Metabolic Engineering ◽  
Hydrogen Production ◽  
Lignocellulosic Biomass ◽  
Caldicellulosiruptor Bescii

scholarly journals Metabolic engineering to enhance bacterial hydrogen production

2007 ◽  
Vol 1 (1) ◽  
pp. 30-39 ◽  
Author(s):  
Toshinari Maeda ◽  
Viviana Sanchez-Torres ◽  
Thomas K. Wood
Keyword(s):  
Metabolic Engineering ◽  
Hydrogen Production

Improvement of hydrogen production by metabolic engineering of Escherichia coli: Modification on both the PTS system and central carbon metabolism

2020 ◽  
Vol 45 (9) ◽  
pp. 5687-5696 ◽  
Author(s):  
Victor E. Balderas-Hernandez ◽  
Kathya P. Landeros Maldonado ◽  
Arturo Sánchez ◽  
Adam Smoliński ◽  
Antonio De Leon Rodriguez
Keyword(s):  
Escherichia Coli ◽  
Metabolic Engineering ◽  
Hydrogen Production ◽  
Carbon Metabolism ◽  
Central Carbon Metabolism ◽  
Central Carbon

Metabolic engineering for enhanced hydrogen production: a review

2013 ◽  
Vol 59 (2) ◽  
pp. 59-78 ◽  
Author(s):  
Yogesh Goyal ◽  
Manish Kumar ◽  
Kalyan Gayen
Keyword(s):  
Metabolic Engineering ◽  
Hydrogen Production ◽  
Hydrogen Gas ◽  
Published Data ◽  
Dual Systems ◽  
Wide Range ◽  
Production Of Hydrogen ◽  
Different Strains ◽  
High Yields ◽  
Associated Species

Hydrogen gas exhibits potential as a sustainable fuel for the future. Therefore, many attempts have been made with the aim of producing high yields of hydrogen gas through renewable biological routes. Engineering of strains to enhance the production of hydrogen gas has been an active area of research for the past 2 decades. This includes overexpression of hydrogen-producing genes (native and heterologous), knockout of competitive pathways, creation of a new productive pathway, and creation of dual systems. Interestingly, genetic mutations in 2 different strains of the same species may not yield similar results. Similarly, 2 different studies on hydrogen productivities may differ largely for the same mutation and on the same species. Consequently, here we analyzed the effect of various genetic modifications on several species, considering a wide range of published data on hydrogen biosynthesis. This article includes a comprehensive metabolic engineering analysis of hydrogen-producing organisms, namely Escherichia coli, Clostridium, and Enterobacter species, and in addition, a short discussion on thermophilic and halophilic organisms. Also, apart from single-culture utilization, dual systems of various organisms and associated developments have been discussed, which are considered potential future targets for economical hydrogen production. Additionally, an indirect contribution towards hydrogen production has been reviewed for associated species.

In vitro metabolic engineering of hydrogen production at theoretical yield from sucrose

2014 ◽  
Vol 24 ◽  
pp. 70-77 ◽  
Author(s):  
Suwan Myung ◽  
Joseph Rollin ◽  
Chun You ◽  
Fangfang Sun ◽  
Sanjeev Chandrayan ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Hydrogen Production ◽  
Theoretical Yield

scholarly journals Application of Metabolic Engineering Approaches in Enhancing Biological Hydrogen Production

2020 ◽  
Author(s):  
Wenfa Ng
Keyword(s):  
Metabolic Engineering ◽  
Hydrogen Production ◽  
Industrial Applications ◽  
Genetically Engineered ◽  
Biohydrogen Production ◽  
Over Expression ◽  
The Past ◽  
Biological Hydrogen Production ◽  
Biological Methods ◽  
Selection Of

Hydrogen is useful as a fuel and could be produced by a variety of means. One approach uses artificial photosynthesis where energy from sunlight powers the splitting of water into hydrogen and oxygen. But, biological methods for producing hydrogen has emerged strongly over the past decades. In particular, specific microorganisms could use different substrates to produce hydrogen at differing yields. Such fundamental discoveries with industrial applications thus motivated the use of metabolic engineering approaches and methodologies in enhancing biological hydrogen production through a series of enzyme over-expression, pathway debottlenecking, and gene deletion. However, such approaches heavily rely on the selection of an appropriate microbial chassis for biohydrogen production. With the proper strain in hand, use of alternative substrates may engender greater hydrogen productivities. But learning from the bioprocessing field, co-culture of two compatible microorganisms have been sought after for improving biohydrogen production. In addition, thermophilic microbes may also be useful candidates for exploiting hydrogen production from composting. Future outlook in the field looks into filling our gaps in understanding of the metabolic network that feeds into hydrogen production in different organisms. But, more importantly, problems such as reduced growth rate in engineered microbes point to fundamental issues with using genetically engineered microorganisms for improved biohydrogen production, to which clever bioprocess engineering may yield solutions.

Sign in / Sign up

Export Citation Format

Share Document