Developing a Dynamics Model for Epidermal Growth Factor (EGF)-Induced Cellular Signaling Events

2017 ◽  
pp. 69-86
Author(s):  
Ning Xi ◽  
Ruiguo Yang ◽  
Bo Song ◽  
King Wai Chiu Lai ◽  
Hongzhi Chen ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Cellular Signaling ◽  
Dynamics Model ◽  
Signaling Events ◽  
Epidermal Growth

scholarly journals Prolactin inhibits epidermal growth factor (EGF)-stimulated signaling events in mouse mammary epithelial cells by altering EGF receptor function.

1993 ◽  
Vol 4 (8) ◽  
pp. 773-780 ◽  
Author(s):  
S E Fenton ◽  
L G Sheffield
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Epithelial Cells ◽  
Egf Receptor ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Mammary Tissue ◽  
Mrna Levels ◽  
Signaling Events ◽  
Epidermal Growth

We have previously shown that lactogenic hormones stimulate epidermal growth factor (EGF) mRNA accumulation in mouse mammary glands in vivo and in mouse mammary epithelial cells (NMuMG line). However, our in vitro studies indicate that the lactogenic hormone prolactin (PRL) completely inhibits EGF-stimulated DNA synthesis. PRL does not alter cholera toxin or insulin-like growth factor-1-stimulated cell growth, thus the inhibition appears to be specific for EGF. Our current studies are designed to evaluate the effects of PRL on EGF-stimulated signaling events in the NMuMG cell line. Cells treated with PRL for 30 min demonstrated a loss of high affinity EGF-binding ability. After long-term PRL treatment (18 h) there was a decrease in EGF receptor (R) number, as determined by [125I]EGF binding. PRL treatment (8 h) also decreased EGF-R mRNA levels. An EGF-stimulated increase in EGF-R mRNA observed 2-4 h after treatment was decreased when PRL was added to the cultures. Furthermore, levels of EGF-stimulated tyrosine phosphorylation of the EGF-R (170 kDa) and phospholipase C gamma (145 kDa) are dramatically decreased in cells treated with PRL. Also of great interest was a decrease in EGF-stimulated c-myc mRNA in PRL-treated cells. We conclude that PRL is acting to down-regulate the EGF-R, thus limiting EGF-stimulated cell signaling in mammary tissue.

Epidermal growth factor enemas are effective in the treatment of left-sided ulcarative colitis

2001 ◽  
Vol 120 (5) ◽  
pp. A11-A12 ◽  
Author(s):  
A SINHA ◽  
J NIGHTINGALE ◽  
K WEST ◽  
R PLAYFORD
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Epidermal Growth

150: The Effect of Epidermal Growth Factor (EGF) on Inward Potassium Rectifying Currents in Bladder Urothelial Cells

2005 ◽  
Vol 173 (4S) ◽  
pp. 41-41
Author(s):  
P. Sean Van Zijl ◽  
Yan Sun ◽  
Mingkui Chen ◽  
Marc Simard ◽  
Toby C. Chai
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Urothelial Cells ◽  
Epidermal Growth
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