In vitro and in vivo Evaluation of Methoxy Polyethylene Glycol–Polylactide Nanoparticles for Small-Molecule Drug Chemotherapy

2014 ◽  
pp. 220-235
Keyword(s):  
Polyethylene Glycol ◽  
Small Molecule ◽  
In Vivo Evaluation ◽  
Small Molecule Drug ◽  
Methoxy Polyethylene Glycol

scholarly journals Controlled Release of Strontium through Neutralization Reaction within a Methoxy(Polyethylene Glycol)-Polyesterc hydrogel

2017 ◽  
Vol 15 (2) ◽  
pp. 162-169 ◽  
Author(s):  
Sydney Peng ◽  
Zhi-Teng Lai ◽  
Ding-Wei Hong ◽  
I-Ming Chu ◽  
Po-Liang Lai
Keyword(s):  
Polyethylene Glycol ◽  
Glycolic Acid ◽  
Time Frame ◽  
Neutralization Reaction ◽  
Bone Void Filler ◽  
Potential Acid ◽  
Methoxy Polyethylene Glycol ◽  
Localized Treatment

Background The aim of this study was to develop a minimally invasive hydrogel system that can release strontium ions, an element that has been shown to increase osteoblast proliferation and prohibit bone resorption, in a controlled manner. Methods SrCO3 was selected as the salt of choice due to potential acid neutralization reaction between SrCO3 and degradation by-products of methoxy(polyethylene glycol)- co-poly(lactic- co-glycolic acid) (mPEG-PLGA): namely, lactic acid and glycolic acid. SrCO3 was incorporated into mPEG-PLGA hydrogel, and the system was assessed for gelation properties, drug release and biocompatibility. Results SrCO3 incorporation at hydrogel to SrCO3 ratios of 5:1, 3:1 and 1:1 (wt%) did not compromise the thermosensitivity of mPEG-PLGA hydrogels. Furthermore, incorporation of SrCO3 at 1:1 ratio prevented copolymer self-catalysis and decreased hydrogel weight loss from 85% to 61% in vitro after 30 days. During the 30-day time frame, zero-order strontium release was observed and was correlated to hydrogel degradation and acidity. The addition of SrCO3 also improved in vivo hydrogel biocompatibility, due to moderation of acidic microenvironment and amelioration of inflammatory response. Conclusions These results showed that the described system is suitable for the extended release of strontium and exhibits potential for localized treatment for osteoporosis or as a bone void filler.

scholarly journals In Vitro and In Vivo Evaluation of a 64Cu-Labeled Polyethylene Glycol-Bombesin Conjugate

2004 ◽  
Vol 19 (1) ◽  
pp. 25-34 ◽  
Author(s):  
Buck E. Rogers ◽  
Debbie Della Manna ◽  
Ahmad Safavy
Keyword(s):  
Polyethylene Glycol ◽  
In Vivo Evaluation

Injectable hydrogels based on MPEG–PCL–RGD and BMSCs for bone tissue engineering

2020 ◽  
Vol 8 (15) ◽  
pp. 4334-4345 ◽  
Author(s):  
Hyun Joo Kim ◽  
Su Jung You ◽  
Dae Hyeok Yang ◽  
Jin Eun ◽  
Hae Kwan Park ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Polyethylene Glycol ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Osteogenic Potential ◽  
Injectable Hydrogels ◽  
Methoxy Polyethylene Glycol

The aim of this study was to investigate the osteogenic potential of BMSCs seeded on RGD-conjugated methoxy polyethylene glycol-polycaprolactone (MP–RGD) in vitro and in vivo.

scholarly journals Physical Gold Nanoparticle-Decorated Polyethylene Glycol-Hydroxyapatite Composites Guide Osteogenesis and Angiogenesis of Mesenchymal Stem Cells

Biomedicines ◽  
2021 ◽  
Vol 9 (11) ◽  
pp. 1632
Author(s):  
Chiung-Chyi Shen ◽  
Shan-Hui Hsu ◽  
Kai-Bo Chang ◽  
Chun-An Yeh ◽  
Hsiang-Chun Chang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Polyethylene Glycol ◽  
Osteogenic Differentiation ◽  
Photoelectron Spectroscopy ◽  
In Vivo Evaluation ◽  
Vitro Assay ◽  
Vis Spectroscopy

In this study, polyethylene glycol (PEG) with hydroxyapatite (HA), with the incorporation of physical gold nanoparticles (AuNPs), was created and equipped through a surface coating technique in order to form PEG-HA-AuNP nanocomposites. The surface morphology and chemical composition were characterized using scanning electron microscopy (SEM), atomic force microscopy (AFM), UV–Vis spectroscopy (UV–Vis), Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and contact angle assessment. The effects of PEG-HA-AuNP nanocomposites on the biocompatibility and biological activity of MC3T3-E1 osteoblast cells, endothelial cells (EC), macrophages (RAW 264.7), and human mesenchymal stem cells (MSCs), as well as the guiding of osteogenic differentiation, were estimated through the use of an in vitro assay. Moreover, the anti-inflammatory, biocompatibility, and endothelialization capacities were further assessed through in vivo evaluation. The PEG-HA-AuNP nanocomposites showed superior biological properties and biocompatibility capacity for cell behavior in both MC3T3-E1 cells and MSCs. These biological events surrounding the cells could be associated with the activation of adhesion, proliferation, migration, and differentiation processes on the PEG-HA-AuNP nanocomposites. Indeed, the induction of the osteogenic differentiation of MSCs by PEG-HA-AuNP nanocomposites and enhanced mineralization activity were also evidenced in this study. Moreover, from the in vivo assay, we further found that PEG-HA-AuNP nanocomposites not only facilitate the anti-immune response, as well as reducing CD86 expression, but also facilitate the endothelialization ability, as well as promoting CD31 expression, when implanted into rats subcutaneously for a period of 1 month. The current research illustrates the potential of PEG-HA-AuNP nanocomposites when used in combination with MSCs for the regeneration of bone tissue, with their nanotopography being employed as an applicable surface modification approach for the fabrication of biomaterials.

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