Micro and Nanotechnology in Vascular Regeneration

2016 ◽  
pp. 717-746 ◽  
Keyword(s):  
Vascular Regeneration

scholarly journals Author Correction: In vivo transduction of ETV2 improves cardiac function and induces vascular regeneration following myocardial infarction

2019 ◽  
Vol 51 (9) ◽  
pp. 1-1 ◽  
Author(s):  
Sunghun Lee ◽  
Dong Hun Lee ◽  
Bong-Woo Park ◽  
Ri Youn Kim ◽  
Anh Duc Hoang ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Cardiac Function ◽  
Vascular Regeneration

scholarly journals Interleukin-17A Triggers the Release of Platelet-Derived Factors Driving Vascular Endothelial Cells toward a Pro-Angiogenic State

Cells ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1855
Author(s):  
Aikaterini Gatsiou ◽  
Kateryna Sopova ◽  
Alexandros Tselepis ◽  
Konstantinos Stellos
Keyword(s):  
Endothelial Cells ◽  
Immune Cell ◽  
Vascular Endothelial Cells ◽  
Interleukin 2 ◽  
Cell Subset ◽  
Aortic Ring ◽  
Inflammatory Factor ◽  
Vascular Endothelial ◽  
Monocyte Chemoattractant Protein 1 ◽  
Vascular Regeneration

Platelets comprise a highly interactive immune cell subset of the circulatory system traditionally known for their unique haemostatic properties. Although platelets are considered as a vault of growth factors, cytokines and chemokines with pivotal role in vascular regeneration and angiogenesis, the exact mechanisms by which they influence vascular endothelial cells (ECs) function remain underappreciated. In the present study, we examined the role of human IL-17A/IL-17RA axis in platelet-mediated pro-angiogenic responses. We reveal that IL-17A receptor (IL-17RA) mRNA is present in platelets transcriptome and a profound increase is documented on the surface of activated platelets. By quantifying the protein levels of several factors, involved in angiogenesis, we identified that IL-17A/IL17RA axis selectively induces the release of vascular endothelial growth factor, interleukin -2 and -4, as well as monocyte chemoattractant protein -1 from treated platelets. However, IL-17A exerted no effect on the release of IL-10, an anti-inflammatory factor with potentially anti-angiogenic properties, from platelets. Treatment of human endothelial cell two-dimensional tubule networks or three-dimensional spheroid and mouse aortic ring structures with IL-17A-induced platelet releasate evoked pro-angiogenic responses of ECs. Our findings suggest that IL-17A may critically affect platelet release of pro-angiogenic factors driving ECs towards a pro-angiogenic state.

scholarly journals Co-Transplantation of Skin-Derived Precursors and Collagen Sponge Facilitates Diabetic Wound Healing by Promoting Local Vascular Regeneration

2015 ◽  
Vol 37 (5) ◽  
pp. 1725-1737 ◽  
Author(s):  
Tingyu Ke ◽  
Mei Yang ◽  
Duo Mao ◽  
Meifeng Zhu ◽  
Yongzhe Che ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Expression Patterns ◽  
Collagen Sponge ◽  
Health Concern ◽  
Diabetic Wound ◽  
Paracrine Signalling ◽  
Vascular Regeneration ◽  
Diabetic Wound Healing

Background/Aims: Impaired diabetes wound healing can often lead to serious complications and remains a major health concern due to the lack of effective therapeutic approaches. Compromised angiogenesis, disrupted growth factor and cytokine activity are all attributable to diabetic wound healing impairment. The skin-derived precursors (SKPs) have been shown to differentiate into vascular and nerve cells, both of which are crucial components for wound repair. Given their easy accessibility and multipotency, the SKPs were proposed as an ideal therapeutic candidate for diabetic wound healing. Since the efficacy of cell therapy is limited by poor cell survival, collagen sponge was employed for better SKPs delivery. Methods: SKPs were isolated and transplanted directly to the wound areas of diabetic mice in the absence and presence of collagen sponge. The effects of SKPs and/or collagen sponge on diabetic wound healing were examined histologically as well as immunostaining of isolectin and α-SMA. Mechanisms via which the SKPs facilitate wound healing were then investigated by transplanting SKPs that have been pre-labelled with a fluorescence dye, Dil. Expression patterns of Dil and an SKP marker, nestin, was also examined. Results and Conclusion: Accelerated wound healing and enhanced local capillary regeneration could be observed 14 days after skin ablation from both SKPs and collagen sponge co-transplanted and collagen sponge only groups. Subsequent analyses further revealed superior pro-angiogenic effects from the SKP and collagen sponge co-delivered group, which are mainly attributable to in vivo transdifferentation and paracrine signalling of the SKPs.

scholarly journals Stem Cell Therapy for Vascular Regeneration

Circulation ◽  
2010 ◽  
Vol 122 (5) ◽  
pp. 517-526 ◽  
Author(s):  
Nicholas J. Leeper ◽  
Arwen L. Hunter ◽  
John P. Cooke
Keyword(s):  
Stem Cell ◽  
Cell Therapy ◽  
Stem Cell Therapy ◽  
Vascular Regeneration

scholarly journals Pivotal Role of Lnk Adaptor Protein in Endothelial Progenitor Cell Biology for Vascular Regeneration

2009 ◽  
Vol 104 (8) ◽  
pp. 969-977 ◽  
Author(s):  
Sang-Mo Kwon ◽  
Takahiro Suzuki ◽  
Atsuhiko Kawamoto ◽  
Masaaki Ii ◽  
Masamichi Eguchi ◽  
...  
Keyword(s):  
Endothelial Progenitor Cell ◽  
Cell Biology ◽  
Progenitor Cell ◽  
Adaptor Protein ◽  
Pivotal Role ◽  
Endothelial Progenitor ◽  
Vascular Regeneration

scholarly journals Age, wound size and position of injury – dependent vascular regeneration assay in growing leaves

2020 ◽  
Author(s):  
Dhanya Radhakrishnan ◽  
Anju Pallipurath Shanmukhan ◽  
Abdul Kareem ◽  
Mabel Maria Mathew ◽  
Vijina Varaparambathu ◽  
...  
Keyword(s):  
Molecular Biology ◽  
Molecular Mechanism ◽  
Rapid Method ◽  
Plant Species ◽  
Plant Parts ◽  
Vascular Regeneration ◽  
New Findings ◽  
Stepwise Method ◽  
Experimental Approaches ◽  
Regeneration Efficiency

ABSTRACTBackgroundRecurring damage to aerial organs of plants necessitates their prompt repair, particularly their vasculature. While vascular regeneration assay in aerial plant parts such as stem and inflorescence stalk are well established, those on leaf vasculature remained unexplored. Recently we established a new vascular regeneration assay in growing leaf and discovered the underlying molecular mechanism.ResultsHere we describe the detailed stepwise method of incision and the regeneration assay used for studying the leaf vascular regeneration. By using a combination of micro-surgical perturbations, brightfield microscopy and other experimental approaches, our new findings show that the regeneration efficiency decreases with aging of the leaf, and increases with the nearness of the wound towards the proximal end of the leaf.ConclusionThis easy-to-master vascular regeneration assay is an efficient and rapid method to study the mechanism of vascular regeneration in growing leaves. It can be readily adapted for other plant species and can be combined with cellular and molecular biology techniques.

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