Biothermodynamics Of Live Cells: Energy Dissipation And Heat Generation In Cellular Cultures

2013 ◽  
pp. 497-566
2018 ◽  
Vol 144 ◽  
pp. 457-468 ◽  
Author(s):  
Wenbo Li ◽  
Xiaojie Zhang ◽  
Yuanyuan Shang ◽  
Qi Chen ◽  
Chunhua Chen ◽  
...  

2008 ◽  
Vol 23 (7) ◽  
pp. 1902-1906 ◽  
Author(s):  
Aijie Han ◽  
Venkata K. Punyamurtula ◽  
Yu Qiao

As a liquid moves in the nanopores of a silica gel, because of the hysteresis of sorption behavior, significant energy dissipation can take place. Through a calometric measurement, the characteristics of associated heat generation are investigated. The temperature variation increases with the mass of silica gel, which consists of a reversible part and an irreversible part. The residual temperature change is about 30% to 60% of the maximum temperature increase and can be accumulated as multiple loading cycles are applied.


2021 ◽  
Vol 1 (74) ◽  
pp. 62-64
Author(s):  
B. Zimin ◽  
A. Khitrina

Observations of a solid body with internal stresses have been carried out, this model allows us to describe the energy dissipation during the change of the elastic stage of deformation to the plastic one. The dependence of heat release on the heat physical properties of the contacting structures is noted.


2018 ◽  
Vol 38 (8) ◽  
pp. 723-729 ◽  
Author(s):  
K. Anas ◽  
Samson David ◽  
R.R. Babu ◽  
M. Selvakumar ◽  
S. Chattopadhyay

AbstractThe dynamic deformation of a viscoelastic material can cause heat generation. This heat generation is an aspect of energy dissipation. The present paper investigates the contribution of crosslink type and density on energy dissipation mechanism in natural rubber compounds. The influences of these elements are investigated using a very high frequency (VHF) analyzer (VHF 104) and a dynamical mechanical analyzer (DMA). The VHF 104 analyzer follows transmissibility and vibration isolation principle, whereas DMA works on dynamical mechanical the deformation principle. Higher crosslink density promotes lower heat generation in rubber compounds. Tanδinterpretation for energy dissipation characteristics of rubbery compounds should be done judiciously to avoid wrong interpretations. Polysulfidic linkages show higher damping ratios (ζ) than monosulfidic or disulfidic linkages due to their dissipative nature. The natural frequency (ωn) of a system at a given mass is the function of its crosslink density.


Author(s):  
Shinya Inoué

This paper reports progress of our effort to rapidly capture, and display in time-lapsed mode, the 3-dimensional dynamic architecture of active living cells and developing embryos at the highest resolution of the light microscope. Our approach entails: (A) real-time video tape recording of through-focal, ultrathin optical sections of live cells at the highest resolution of the light microscope; (B) repeat of A at time-lapsed intervals; (C) once each time-lapsed interval, an image at home focus is recorded onto Optical Disk Memory Recorder (OMDR); (D) periods of interest are selected using the OMDR and video tape records; (E) selected stacks of optical sections are converted into plane projections representing different view angles (±4 degrees for stereo view, additional angles when revolving stereos are desired); (F) analysis using A - D.


Author(s):  
E. D. Salmon ◽  
J. C. Waters ◽  
C. Waterman-Storer

We have developed a multi-mode digital imaging system which acquires images with a cooled CCD camera (Figure 1). A multiple band pass dichromatic mirror and robotically controlled filter wheels provide wavelength selection for epi-fluorescence. Shutters select illumination either by epi-fluorescence or by transmitted light for phase contrast or DIC. Many of our experiments involve investigations of spindle assembly dynamics and chromosome movements in live cells or unfixed reconstituted preparations in vitro in which photodamage and phototoxicity are major concerns. As a consequence, a major factor in the design was optical efficiency: achieving the highest image quality with the least number of illumination photons. This principle applies to both epi-fluorescence and transmitted light imaging modes. In living cells and extracts, microtubules are visualized using X-rhodamine labeled tubulin. Photoactivation of C2CF-fluorescein labeled tubulin is used to locally mark microtubules in studies of microtubule dynamics and translocation. Chromosomes are labeled with DAPI or Hoechst DNA intercalating dyes.


Author(s):  
K.I. Pagh ◽  
M.R. Adelman

Unicellular amoebae of the slime mold Physarum polycephalum undergo marked changes in cell shape and motility during their conversion into flagellate swimming cells (l). To understand the processes underlying motile activities expressed during the amoebo-flagellate transformation, we have undertaken detailed investigations of the organization, formation and functions of subcellular structures or domains of the cell which are hypothesized to play a role in movement. One focus of our studies is on a structure, termed the “ridge” which appears as a flattened extension of the periphery along the length of transforming cells (Fig. 1). Observations of live cells using Nomarski optics reveal two types of movement in this region:propagation of undulations along the length of the ridge and formation and retraction of filopodial projections from its edge. The differing activities appear to be associated with two characteristic morphologies, illustrated in Fig. 1.


Author(s):  
Claude Jaupart ◽  
Jean-Claude Mareschal
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