Monosaccharide Analysis of Recombinant Glycoproteins by High pH Anion-Exchange Chromatography/High-Performance Liquid Chromatography Using a Refractive Index Detector

1997 ◽  
pp. 481-498
Keyword(s):  
Refractive Index ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Anion Exchange ◽  
High Performance ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
High Ph ◽  
Recombinant Glycoproteins ◽  
Refractive Index Detector

Anion-exchange chromatography of proteins on AG MP-1 using high-performance liquid chromatography equipment

1984 ◽  
Vol 142 (2) ◽  
pp. 373-377 ◽  
Author(s):  
John T. Axelson ◽  
James W. Bodley ◽  
Jeou-Yuan Chen ◽  
Patricia C. Dunlop ◽  
Luann P. Rosenthal ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Anion Exchange ◽  
High Performance ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography

Comparison of different anion-exchange chromatography resins for the purification of cyanobacterial microcystins

2015 ◽  
Vol 16 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Theerasak Somdee ◽  
Anchana Somdee
Keyword(s):  
Liquid Chromatography ◽  
Anion Exchange ◽  
High Performance ◽  
Total Yield ◽  
Deae Cellulose ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Ionization Mass ◽  
Freeze Dried ◽  
Wide Range

For the first time, different types of diethylaminoethyl (DEAE) anion-exchange resins, widely used in previous studies, were investigated to determine the most effective resin for the purification of microcystins (MCs). MCs were extracted from freeze-dried Microcystis aeruginosa cells that had been harvested from the Bueng Nong Khot reservoir, Khon Kaen, Thailand. The toxins were precipitated with ammonium sulfate and then fractionated using five different anion-exchange chromatography resins, followed by chromatography with a C18 cartridge. The toxins were further identified via liquid chromatography–electrospray ionization–mass spectrometry (LC-ESI-MS) analysis, and the yields and purity were determined by high-performance liquid chromatography (HPLC) with ultraviolet detection. DEAE Sephadex A-25 exhibited the best overall performance for MC purification regarding both yield and purity, followed by DEAE cellulose, DEAE Sephacel, DEAE Sepharose Fast Flow and Toyopearl DEAE. Four MC variants, MC-RR, MC-FR, [Dha7]MC-LR and MC-WR, were obtained, and [Dha7]MC-LR was the major variant, with a total yield of 53.08 mg and a purity of 95% using the Sephadex resin. This study indicates that protein precipitation and single-column chromatography using DEAE Sephadex A-25 constitute an effective method for the purification of a wide range of MC variants.

scholarly journals High-Performance Anion-Exchange Chromatography Coupled with Pulsed Electrochemical Detection as a Powerful Tool to Evaluate Carbohydrates of Food Interest: Principles and Applications

2012 ◽  
Vol 2012 ◽  
pp. 1-13 ◽  
Author(s):  
Claudio Corradini ◽  
Antonella Cavazza ◽  
Chiara Bignardi
Keyword(s):  
Anion Exchange ◽  
Electrochemical Detection ◽  
High Performance ◽  
Oxidation Mechanism ◽  
Amperometric Detection ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
High Ph ◽  
Analytical Technique ◽  
Pulsed Electrochemical Detection

Specific HPLC approaches are essential for carbohydrate characterization in food products. Carbohydrates are weak acids with pKa values in the range 12–14 and, consequently, at high pH can be transformed into oxyanions, and can be readily separated using highly efficient anion-exchange columns. Electrochemical detection in HPLC has been proven to be a powerful analytical technique for the determination of compounds containing electroactive groups; pulsed amperometric detection of carbohydrates is favourably performed by taking advantage of their electrocatalytic oxidation mechanism at a gold working electrode in a basic media. High-performance Anion Exchange Chromatography (HPAEC) at high pH coupled with pulsed electrochemical detection (PED) is one of the most useful techniques for carbohydrate determination either for routine monitoring or research application. This technique has been of a great impact on the analysis of oligo- and polysaccharides. The compatibility of electrochemical detection with gradient elution, coupled with the high selectivity of the anion-exchange stationary phases, allows mixtures of simple sugars, oligo- and polysaccharides to be separated with high resolution in a single run. A few reviews have been written on HPAEC-PED of carbohydrates of food interest in the last years. In this paper the recent developments in this field are examined.

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