High-Resolution AFM Imaging of Native Biological Membranes

2013 ◽  
Author(s):  
Lu-Ning Liu ◽  
Simon Scheuring
Keyword(s):  
High Resolution ◽  
Biological Membranes ◽  
Afm Imaging

Imaging of Xenopus laevis Oocyte Plasma Membrane in Physiological-Like Conditions by Atomic Force Microscopy

2013 ◽  
Vol 19 (5) ◽  
pp. 1358-1363 ◽  
Author(s):  
Massimo Santacroce ◽  
Federica Daniele ◽  
Andrea Cremona ◽  
Diletta Scaccabarozzi ◽  
Michela Castagna ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Plasma Membrane ◽  
High Resolution ◽  
Membrane Proteins ◽  
Xenopus Laevis ◽  
Functional Study ◽  
Xenopus Laevis Oocyte ◽  
Force Microscopy ◽  
Atomic Force ◽  
Afm Imaging

AbstractXenopus laevis oocytes are an interesting model for the study of many developmental mechanisms because of their dimensions and the ease with which they can be manipulated. In addition, they are widely employed systems for the expression and functional study of heterologous proteins, which can be expressed with high efficiency on their plasma membrane. Here we applied atomic force microscopy (AFM) to the study of the plasma membrane of X. laevis oocytes. In particular, we developed and optimized a new sample preparation protocol, based on the purification of plasma membranes by ultracentrifugation on a sucrose gradient, to perform a high-resolution AFM imaging of X. laevis oocyte plasma membrane in physiological-like conditions. Reproducible AFM topographs allowed visualization and dimensional characterization of membrane patches, whose height corresponds to a single lipid bilayer, as well as the presence of nanometer structures embedded in the plasma membrane and identified as native membrane proteins. The described method appears to be an applicable tool for performing high-resolution AFM imaging of X. laevis oocyte plasma membrane in a physiological-like environment, thus opening promising perspectives for studying in situ cloned membrane proteins of relevant biomedical/pharmacological interest expressed in this biological system.

High resolution non-contact AFM imaging of liquids condensed onto chemically nanopatterned surfaces

2006 ◽  
Vol 106 (8-9) ◽  
pp. 703-708 ◽  
Author(s):  
Antonio Checco ◽  
Yuguang Cai ◽  
Oleg Gang ◽  
Benjamin M. Ocko
Keyword(s):  
High Resolution ◽  
Afm Imaging

scholarly journals Hydration Dynamics and the Future of Small-Amplitude AFM Imaging in Air

Molecules ◽  
2021 ◽  
Vol 26 (23) ◽  
pp. 7083
Author(s):  
Sergio Santos ◽  
Tuza A. Olukan ◽  
Chia-Yun Lai ◽  
Matteo Chiesa
Keyword(s):  
High Resolution ◽  
Small Amplitude ◽  
Stable State ◽  
High Resolution Imaging ◽  
Hydration Layer ◽  
Afm Imaging ◽  
The Future ◽  
Resolution Imaging ◽  
Tip Radius ◽  
Hydration Dynamics

Here, we discuss the effects that the dynamics of the hydration layer and other variables, such as the tip radius, have on the availability of imaging regimes in dynamic AFM—including multifrequency AFM. Since small amplitudes are required for high-resolution imaging, we focus on these cases. It is possible to fully immerse a sharp tip under the hydration layer and image with amplitudes similar to or smaller than the height of the hydration layer, i.e., ~1 nm. When mica or HOPG surfaces are only cleaved, molecules adhere to their surfaces, and reaching a thermodynamically stable state for imaging might take hours. During these first hours, different possibilities for imaging emerge and change, implying that these conditions must be considered and reported when imaging.

scholarly journals A Correlative Defect Analyzer Combining Glide Test with Atomic Force Microscope

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Jizhong He
Keyword(s):  
High Resolution ◽  
Atomic Force Microscope ◽  
Optical Imaging ◽  
Hard Disk Drive ◽  
Disk Drive ◽  
Atomic Force ◽  
Typical Data ◽  
Afm Imaging ◽  
Afm Analysis

We have developed a novel instrument combining a glide tester with an Atomic Force Microscope (AFM) for hard disk drive (HDD) media defect test and analysis. The sample stays on the same test spindle during both glide test and AFM imaging without losing the relevant coordinates. This enables an in situ evaluation with the high-resolution AFM of the defects detected by the glide test. The ability for the immediate follow-on AFM analysis solves the problem of relocating the defects quickly and accurately in the current workflow. The tool is furnished with other functions such as scribing, optical imaging, and head burnishing. Typical data generated from the tool are shown at the end of the paper. It is further demonstrated that novel experiments can be carried out on the platform by taking advantage of the correlative capabilities of the tool.

scholarly journals Mechanism of high-resolution STM/AFM imaging with functionalized tips

2014 ◽  
Vol 90 (8) ◽  
Author(s):  
Prokop Hapala ◽  
Georgy Kichin ◽  
Christian Wagner ◽  
F. Stefan Tautz ◽  
Ruslan Temirov ◽  
...  
Keyword(s):  
High Resolution ◽  
Afm Imaging ◽  
Functionalized Tips
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