Detrusor smooth muscle physiology

2004 ◽  
pp. 307-330
Keyword(s):  
Smooth Muscle ◽  
Muscle Physiology ◽  
Detrusor Smooth Muscle

scholarly journals Novel role for the transient potential receptor melastatin 4 channel in guinea pig detrusor smooth muscle physiology

2013 ◽  
Vol 304 (5) ◽  
pp. C467-C477 ◽  
Author(s):  
Amy C. Smith ◽  
Kiril L. Hristov ◽  
Qiuping Cheng ◽  
Wenkuan Xin ◽  
Shankar P. Parajuli ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Transient Receptor Potential ◽  
Critical Role ◽  
Protein Detection ◽  
Muscle Physiology ◽  
Detrusor Smooth Muscle ◽  
Excitation Contraction Coupling ◽  
Contraction Coupling ◽  
Trpm4 Channel

Members of the transient receptor potential (TRP) channel superfamily, including the Ca2+-activated monovalent cation-selective TRP melastatin 4 (TRPM4) channel, have been recently identified in the urinary bladder. However, their expression and function at the level of detrusor smooth muscle (DSM) remain largely unexplored. In this study, for the first time we investigated the role of TRPM4 channels in guinea pig DSM excitation-contraction coupling using a multidisciplinary approach encompassing protein detection, electrophysiology, live-cell Ca2+ imaging, DSM contractility, and 9-phenanthrol, a recently characterized selective inhibitor of the TRPM4 channel. Western blot and immunocytochemistry experiments demonstrated the expression of the TRPM4 channel in whole DSM tissue and freshly isolated DSM cells with specific localization on the plasma membrane. Perforated whole cell patch-clamp recordings and real-time Ca2+ imaging experiments with fura 2-AM, both using freshly isolated DSM cells, revealed that 9-phenanthrol (30 μM) significantly reduced the cation current and decreased intracellular Ca2+ levels. 9-Phenanthrol (0.1–30 μM) significantly inhibited spontaneous, 0.1 μM carbachol-induced, 20 mM KCl-induced, and nerve-evoked contractions in guinea pig DSM-isolated strips with IC50 values of 1–7 μM and 70–80% maximum inhibition. 9-Phenanthrol also reduced nerve-evoked contraction amplitude induced by continuous repetitive electrical field stimulation of 10-Hz frequency and shifted the frequency-response curve (0.5–50 Hz) relative to the control. Collectively, our data demonstrate the novel finding that TRPM4 channels are expressed in guinea pig DSM and reveal their critical role in the regulation of guinea pig DSM excitation-contraction coupling.

257: The Phosphodiesterase Type 4 Inhibitor, Rolipram, is More Efficient to Relax Detrusor Smooth Muscle in Rats with Overactive Bladder than in Control Rats

2007 ◽  
Vol 177 (4S) ◽  
pp. 86-86
Author(s):  
Stephanie Oger ◽  
Delphine Behr-Roussel ◽  
Katell Mevel ◽  
Jacques Bernabe ◽  
Pierre Denys ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Overactive Bladder ◽  
Detrusor Smooth Muscle ◽  
Phosphodiesterase Type

373: The Contractile Properties of Detrusor Smooth Muscle in Cloacal Exstrophy

2007 ◽  
Vol 177 (4S) ◽  
pp. 124-124
Author(s):  
Navroop S. Johal ◽  
Peter M. Cuckow ◽  
Christopher H. Fry
Keyword(s):  
Smooth Muscle ◽  
Contractile Properties ◽  
Detrusor Smooth Muscle ◽  
Cloacal Exstrophy

MEASUREMENT OF INTERCELLULAR ELECTRICAL COUPLING IN GUINEA-PIG DETRUSOR SMOOTH MUSCLE

1999 ◽  
Vol 161 (2) ◽  
pp. 660-664 ◽  
Author(s):  
C.H. FRY ◽  
M. COOKLIN ◽  
J. BIRNS ◽  
A.R. MUNDY
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Electrical Coupling ◽  
Detrusor Smooth Muscle

Effect of low concentrations of hydrogen peroxide on the contractile responses of rat detrusor smooth muscle strips

2010 ◽  
Vol 638 (1-3) ◽  
pp. 115-120 ◽  
Author(s):  
June Hyun Han ◽  
Moo Yeol Lee ◽  
Shin Young Lee ◽  
In Ho Chang ◽  
Hae Jong Kim ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Smooth Muscle ◽  
Detrusor Smooth Muscle ◽  
Contractile Responses ◽  
Low Concentrations
Sign in / Sign up

Export Citation Format

Share Document