Human embryonic development in vitro

2006 ◽  
pp. 316-328
Keyword(s):  
Embryonic Development ◽  
Development In Vitro

scholarly journals Nobiletin enhances the development and quality of bovine embryos in vitro during two key periods of embryonic genome activation

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Karina Cañón-Beltrán ◽  
Yulia N. Cajas ◽  
Serafín Peréz-Cerezales ◽  
Claudia L. V. Leal ◽  
Ekaitz Agirregoitia ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Signaling Pathway ◽  
Akt Signaling ◽  
Mitochondrial Activity ◽  
Bovine Embryos ◽  
Akt Signaling Pathway ◽  
Cell Stage ◽  
Development In Vitro

AbstractIn vitro culture can alter the development and quality of bovine embryos. Therefore, we aimed to evaluate whether nobiletin supplementation during EGA improves embryonic development and blastocyst quality and if it affects PI3K/AKT signaling pathway. In vitro zygotes were cultured in SOF + 5% FCS (Control) or supplemented with 5, 10 or 25 µM nobiletin (Nob5, Nob10, Nob25) or with 0.03% dimethyl-sulfoxide (CDMSO) during minor (2 to 8-cell stage; MNEGA) or major (8 to 16-cell stage; MJEGA) EGA phase. Blastocyst yield on Day 8 was higher in Nob5 (42.7 ± 1.0%) and Nob10 (44.4 ± 1.3%) for MNEGA phase and in Nob10 (61.0 ± 0.8%) for MJEGA phase compared to other groups. Mitochondrial activity was higher and lipid content was reduced in blastocysts produced with nobiletin, irrespective of EGA phase. The mRNA abundance of CDK2, H3-3B, H3-3A, GPX1, NFE2L2 and PPARα transcripts was increased in 8-cells, 16-cells and blastocysts from nobiletin groups. Immunofluorescence analysis revealed immunoreactive proteins for p-AKT forms (Thr308 and Ser473) in bovine blastocysts produced with nobiletin. In conclusion, nobiletin supplementation during EGA has a positive effect on preimplantation bovine embryonic development in vitro and corroborates on the quality improvement of the produced blastocysts which could be modulated by the activation of AKT signaling pathway.

High doses of lipid-core nanocapsules do not affect bovine embryonic development in vitro

2017 ◽  
Vol 45 ◽  
pp. 194-201 ◽  
Author(s):  
Caroline G. Lucas ◽  
Mariana H. Remião ◽  
Franciele A. Bruinsmann ◽  
Isadora A.R. Lopes ◽  
Morgana A. Borges ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Lipid Core ◽  
Development In Vitro ◽  
High Doses

scholarly journals Adverse reproductive effects of S100A9 on bovine sperm and early embryonic development in vitro

PLoS ONE ◽  
2020 ◽  
Vol 15 (1) ◽  
pp. e0227885 ◽  
Author(s):  
Natsumi Funeshima ◽  
Nao Tanikawa ◽  
Hikari Yaginuma ◽  
Hiroyuki Watanabe ◽  
Hisataka Iwata ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Early Embryonic Development ◽  
Bovine Sperm ◽  
Reproductive Effects ◽  
Development In Vitro

Effect of the number of inseminated spermatozoa on subsequent human and mouse embryonic development in vitro

1992 ◽  
Vol 7 (7) ◽  
pp. 1010-1013 ◽  
Author(s):  
John C.M. Dumoulin ◽  
Marijke Bras ◽  
Jolande A. Land ◽  
Math H.E.C. Pieters ◽  
M.Engin Enginsu ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Development In Vitro ◽  
Human And Mouse

Effects of Inflammatory Conditions from Coculture with Activated Macrophages on Mouse Preimplantation Embryonic Development In Vitro.

2011 ◽  
Vol 85 (Suppl_1) ◽  
pp. 712-712
Author(s):  
Jin Hyun Jun ◽  
Jae Wang Lee ◽  
Sung Yong Kim ◽  
You-Jin Jeong ◽  
Hee-Gyoo Kang
Keyword(s):  
Embryonic Development ◽  
Activated Macrophages ◽  
Inflammatory Conditions ◽  
Development In Vitro

scholarly journals Effects of bovine spermatozoa preparation on embryonic development in vitro

2006 ◽  
Vol 4 (1) ◽  
Author(s):  
Marko Samardzija ◽  
Martina Karadjole ◽  
Iva Getz ◽  
Zdenko Makek ◽  
Marijan Cergolj ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Bovine Spermatozoa ◽  
Development In Vitro

Factors Influencing Human Embryonic Development in Vitro

1985 ◽  
Vol 442 (1 In Vitro Fert) ◽  
pp. 342-356 ◽  
Author(s):  
SIMON B. FISHEL ◽  
JACQUES COHEN ◽  
CAROLE FEHILLY ◽  
JEAN M. PURDY ◽  
D. EUROF WALTERS ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Factors Influencing ◽  
Development In Vitro

scholarly journals Embryonic development in vitro is compromised by the ICSI procedure

2000 ◽  
Vol 15 (7) ◽  
pp. 1592-1596 ◽  
Author(s):  
T.A. Griffiths ◽  
A.P. Murdoch ◽  
M. Herbert
Keyword(s):  
Embryonic Development ◽  
Development In Vitro
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