Use of Chondrogenic Progenitor Cells in Osteoarthritis

2013 ◽  
pp. 231-244
Author(s):  
Boris Schminke ◽  
Nicolai Miosge ◽  
Hayat Muhammad
Keyword(s):  
Progenitor Cells ◽  
Chondrogenic Progenitor Cells

Migrating Progenitor Cells Derived From Injured Cartilage Surface Respond to Damage-Associated Molecular Patterns

Cartilage ◽  
2021 ◽  
pp. 194760352110495
Author(s):  
Lei Ding ◽  
Cheng Zhou ◽  
Hongjun Zheng ◽  
Quanming Wang ◽  
Haiyan Song ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Progenitor Cells ◽  
Quantitative Polymerase Chain Reaction ◽  
Critical Role ◽  
Cartilage Degeneration ◽  
Expression Levels ◽  
Chondrogenic Progenitor Cells ◽  
The Difference ◽  
Molecular Patterns ◽  
Damage Associated Molecular Patterns

Objective: To delineate the response of migrating chondrogenic progenitor cells (CPCs) that arose from the surface of mechanically injured articular cartilage to proinflammatory damage-associated-molecular-patterns (DAMPs). Design: Bovine CPCs and non-CPC chondrocytes isolated from either impacted or scratched articular cartilage were studied. Those 2 types of cells were treated with mitochondrial DAMPs (MTDs; 10 nM fMLF and 10 µg/mL CpG DNA), or 10 nM HMGB1, or 10 ng/mL IL-1b for 24 hours. At the end of experiments, conditioned media and cell lysates were collected for analysis of expression levels of matrix metalloproteinases (MMPs), chemokines, and cytokines that are associated with cartilage degeneration with Western blotting and quantitative polymerase chain reaction. The difference of expression levels was compared by Welch’s t-test. Results: Our data indicated that HMGB1 and MTDs remarkably upregulated pro-MMP-13 expression in CPCs. Compared with non-CPCs, CPCs expressed significantly more baseline mRNAs of MMP-13, CXCL12, and IL-6. MTDs greatly increased the expression of MMP-13 and IL-6 in CPCs by over 100-fold ( P < 0.001). MTDs also significantly increased IL-8 expression in CPCs to a similar extent ( P < 0.001). However, when IL-1b was present, CPCs expressed less MMP-3 and active MMP-13 proteins as well as less CCL2 and IL-6 than did non-CPCs. Conclusions: We concluded that CPCs were more sensitive than non-CPCs in response to DAMPs, especially MTDs. The proinflammatory nature of CPCs implied their critical role in the early phase of posttraumatic osteoarthritis development.

Epidemiology of chondrogenic progenitor cells resident in tissues around osteoarthritic knee

Cytotherapy ◽  
2018 ◽  
Vol 20 (5) ◽  
pp. S58
Author(s):  
V.R. Mantripragada ◽  
W. Bova ◽  
C. Boehm ◽  
N.S. Piuzzi ◽  
R. Midura ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Osteoarthritic Knee ◽  
Chondrogenic Progenitor Cells

scholarly journals Biological potential alterations of migratory chondrogenic progenitor cells during knee osteoarthritic progression

2020 ◽  
Vol 22 (1) ◽  
Author(s):  
Yu-Xing Wang ◽  
Zhi-Dong Zhao ◽  
Qian Wang ◽  
Zhong-Li Li ◽  
Ya Huang ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Biological Potential ◽  
Chondrogenic Progenitor Cells

scholarly journals Biological Potential Alterations of Migratory Chondrogenic Progenitor Cells during Knee Osteoarthritic Progression

2019 ◽  
Author(s):  
Yu-Xing Wang ◽  
Zhi-Dong Zhao ◽  
Qian Wang ◽  
Zhong-Li Li ◽  
Ya Huang ◽  
...  
Keyword(s):  
Cell Migration ◽  
Progenitor Cells ◽  
Morphological Characteristics ◽  
Histochemical Staining ◽  
Osteoarthritic Cartilage ◽  
Mrna Sequencing ◽  
Biological Potential ◽  
Chondrogenic Progenitor Cells ◽  
Articular Cartilages ◽  
Grade 3

Abstract Background: Although studies have demonstrated that chondrogenic progenitor cells (CPCs) remain present in human osteoarthritic cartilage, the heterogeneity of CPCs subpopulations, the biological alterations of CPCs, and their contributions to the progression of osteoarthritis remain to be investigated. Methods: CPCs were isolated from paired grade 1-2 and grade 3-4 osteoarthritic cartilage by virtue of cell migratory capacities. The cell morphology, immunophenotype, self-renewal, multidifferentiation, and cell migration of these CPCs were evaluated. Additionally, the distributions of CPCs in articular cartilage were determined by immuno-histochemical staining. Furthermore, a high-through mRNA sequencing was performed to explore the underlying mechanisms. Results: Migratory CPCs (mCPCs) robustly outgrew from collagenases-digested osteoarthritic cartilages for 2 weeks after the initial culture. The mCPCs from grade 3-4 cartilages (mCPCs, grade 3-4) harbored morphological characteristics, cell proliferation and colony formation capacity that were similar to those of the mCPCs from the grade 1-2 cartilages (mCPCs, grade 1-2). However, the mCPCs (grade 3-4) highly expressed CD271. In addition, the mCPCs (grade 3-4) showed enhanced osteo-adipogenic activities and decreased chondrogenic capacity. Furthermore, the mCPCs (grade 3-4) exhibited stronger cell migration in response to osteoarthritis synovial fluids. More CD105+ cells resided in grade 3-4 superficial articular cartilages. Moreover, the results of mRNA sequencing showed that mCPCs (grade 3-4) expressed higher migratory molecules. Conclusions: Our data suggest that more mCPCs (grade 3-4) migrate to injured articular cartilages but with decreased cartilage-repairing capacity, which might accelerate cartilage degradation. Thus, these dysfunctional mCPCs might be a novel cell target to alleviate cartilage lesions.

scholarly journals Biological Potential Alterations of Migratory Chondrogenic Progenitor Cells during Knee Osteoarthritic Progression

2020 ◽  
Author(s):  
Yu-Xing Wang ◽  
Zhi-Dong Zhao ◽  
Qian Wang ◽  
Zhong-Li Li ◽  
Ya Huang ◽  
...  
Keyword(s):  
Cell Migration ◽  
Progenitor Cells ◽  
Morphological Characteristics ◽  
Osteoarthritic Cartilage ◽  
Medial Condyle ◽  
Mrna Sequencing ◽  
Biological Potential ◽  
Chondrogenic Progenitor Cells ◽  
Articular Cartilages ◽  
Grade 3

Abstract Background: Although increasing studies have demonstrated that chondrogenic progenitor cells (CPCs) remain present in human osteoarthritic cartilage, the biological alterations of the CPCs from the less diseased lateral tibial condyle and the more diseased medial condyle of same patient remain to be investigated. Methods: CPCs were isolated from paired grade 1-2 and grade 3-4 osteoarthritic cartilage by virtue of cell migratory capacities. The cell morphology, immunophenotype, self-renewal, multi-differentiation, and cell migration of these CPCs were evaluated. Additionally, the distributions of CD105+/CD271+ cells in OA osteochondral specimen were determined. Furthermore, a high-through mRNA sequencing was performed. Results: Migratory CPCs (mCPCs) robustly outgrew from mildly collagenases-digested osteoarthritic cartilages. The mCPCs from grade 3-4 cartilages (mCPCs, grade 3-4) harbored morphological characteristics, cell proliferation and colony formation capacity that were similar to those of the mCPCs from the grade 1-2 OA cartilages (mCPCs, grade 1-2). However, the mCPCs (grade 3-4) highly expressed CD271. In addition, the mCPCs (grade 3-4) showed enhanced osteo-adipogenic activities and decreased chondrogenic capacity. Furthermore, the mCPCs (grade 3-4) exhibited stronger cell migration in response to osteoarthritis synovial fluids. More CD105+/CD271+ cells resided in grade 3-4 articular cartilages. Moreover, the results of mRNA sequencing showed that mCPCs (grade 3-4) expressed higher migratory molecules. Conclusions: Our data suggest that more mCPCs (grade 3-4) migrate to injured articular cartilages but with enhanced osteo-adipogenic and decreased chondrogenic capacity, which might explain the pathological changes of mCPCs during the progression of OA from early to late stages. Thus, these dysfunctional mCPCs might be optional cell targets for OA therapies.

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