Umbilical Cord Matrix Cells—Promising Instrument for Regenerative Medicine

2013 ◽  
pp. 212-227
Author(s):  
Maslova O. ◽  
Deryabina G. ◽  
Kordium A.
Keyword(s):  
Regenerative Medicine ◽  
Umbilical Cord ◽  
Umbilical Cord Matrix

scholarly journals POSTNATAL EXTRA-EMBRYONIC TISSUES AS A SOURCE OF MULTIPLE CELL TYPES FOR REGENERATIVE MEDICINE APPLICATIONS

2017 ◽  
Vol 39 (3) ◽  
pp. 186-190 ◽  
Author(s):  
O S Gubar ◽  
A I Rodnichenko ◽  
R G Vasylie ◽  
A V Zlatska ◽  
D O Zubov
Keyword(s):  
Regenerative Medicine ◽  
Umbilical Cord ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Umbilical Vein ◽  
Cell Types ◽  
Population Doubling ◽  
Embryonic Tissues ◽  
Chorionic Membrane ◽  
Multiple Cell

Aim: We aimed to isolate and characterize the cell types which could be obtained from postnatal extra-embryonic tissues. Materials and Methods: Fresh tissues (no more than 12 h after delivery) were used for enzymatic or explants methods of cell isolation. Obtained cultures were further maintained at 5% oxygen. At P3 cell phenotype was assessed by fluorescence-activated cell sorting, population doubling time was calculated and the multilineage differentiation assay was performed. Results: We have isolated multiple cell types from postnatal tissues. Namely, placental mesenchymal stromal cells from placenta chorionic disc, chorionic membrane mesenchymal stromal cells (ChM-MSC) from free chorionic membrane, umbilical cord MSC (UC-MSC) from whole umbilical cord, human umbilical vein endothelial cells (HUVEC) from umbilical vein, amniotic epithelial cells (AEC) and amniotic MSC (AMSC) from amniotic membrane. All isolated cell types displayed high proliferation rate together with the typical MSC phenotype: CD73+CD90+CD105+CD146+CD166+CD34-CD45-HLA-DR-. HUVEC constitutively expressed key markers CD31 and CD309. Most MSC and AEC were capable of osteogenic and adipogenic differentiation. Conclusion: We have shown that a wide variety of cell types can be easily isolated from extra-embryonic tissues and expanded ex vivo for regenerative medicine applications. These cells possess typical MSC properties and can be considered an alternative for adult MSC obtained from bone marrow or fat, especially for allogeneic use.

132 UMBILICAL CORD MATRIX STEM CELLS: A NEW SOURCE FOR GENERATION OF HEPATOCYTE-LIKE CELLS

2008 ◽  
Vol 48 ◽  
pp. S57
Author(s):  
D. Campard ◽  
P.A. Lysy ◽  
N. Jazouli ◽  
F. Andre ◽  
M. Najimi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Umbilical Cord ◽  
Umbilical Cord Matrix
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