General factors influencing ovarian function and the prognosis for ovulation induction

2005 ◽  
pp. 33-38
Keyword(s):  
Ovulation Induction ◽  
Ovarian Function ◽  
Factors Influencing

Monitoring Induction of Ovulation by Rapid Radioimmunoassays of Oestrogen and Pregnanediol Glucuronides

1981 ◽  
Vol 18 (6) ◽  
pp. 343-349 ◽  
Author(s):  
A R Maclean ◽  
K H Outch ◽  
J M Russell ◽  
J B Brown ◽  
P M Dennis
Keyword(s):  
Liquid Chromatography ◽  
Induction Therapy ◽  
Ovulation Induction ◽  
Ovarian Function ◽  
Ovarian Response ◽  
Gas Liquid Chromatography ◽  
Excellent Correlation ◽  
Sample Handling ◽  
Induction Of Ovulation

To increase the sample-handling capacity for an induction of ovulation programme, direct urinary radioimmunoassays (RIA) for three steroid glucuronides, pregnanediol-3-glucuronide (Pd-3-G), oestrone-3-glucuronide (E1-3-G), and oestriol-16α-glucuronide (E3-16-G), were established. Results obtained for urinary Pd-3-G measured by direct RIA show an excellent correlation (r = 0·98, N = 46) with those for urinary pregnanediol measured by gas-liquid chromatography. Estrone-3-glucuronide (E1-3-G) and estriol-16α-glucuronide (E3-16-G) values, measured by direct RIA, closely paralleled the total urinary oestrogen measured fluorimetrically. Ovarian response to ovulation induction therapy can be monitored by observing the changes in the levels of E1-3-G in urine. Pre-ovulatory levels of urinary E3-16-G were found to be too low for use in this regard. Direct RIA for E1-3-G and Pd-3-G are recommended as reliable indices of ovarian function in the monitoring of patients receiving treatment for the induction of ovulation.

132 A NEW APPROACH TO ASSESS OVARIAN STRUCTURES IN LIVE RABBITS: SURGICAL TRANSLOCATION AND ULTRASOUND BIOMICROSCOPY

2012 ◽  
Vol 24 (1) ◽  
pp. 178
Author(s):  
M. P. Cervantes ◽  
J. M. Palomino ◽  
B. Toosi ◽  
K. Linn ◽  
G. P. Adams
Keyword(s):  
Ovulation Induction ◽  
Ovarian Function ◽  
Subcutaneous Fat ◽  
Ultrasound Biomicroscopy ◽  
The Body ◽  
Skin Thickness ◽  
Sound Waves ◽  
New Approach ◽  
Factors Associated

For the purpose of determining factors associated with ovulation induction in rabbits, there is a need to examine the ovaries in a serial fashion. To this end, a study was designed to develop an approach that would permit serial evaluation of ovarian structures in vivo using ultrasound biomicroscopy (UBM) while preserving ovarian function in rabbits. The use of UBM has enabled characterisation of ovarian dynamics in live mice and has recently been validated for assessing structures in excised rabbit ovaries (Cervantes et al. 2011 Reprod. Fertil. Dev. 23, 183–184). However, acoustic impedance of the body wall prevented acquisition of high-quality ovarian images in vivo by transabdominal UBM in rabbits. Female New Zealand White rabbits (5 months old; n = 12) underwent surgical ovarian translocation. Each ovary was exteriorized through a lateral flank incision on the respective sides, without compromising vascular supply. The mesovarium at each pole of the ovary was transfixed to the muscle layers of the abdominal wall to keep the ovary in a subcutaneous position. Finally, the subcutaneous and skin layers were closed. No postoperative complications were observed. The surgical site was allowed to heal for 2 weeks and the skin sutures were removed before UBM was attempted. The rabbits were wrapped in a towel for restraint without sedation. Acoustic gel was applied to the shaved skin over the translocated ovary and ovarian structures were imaged transcutaneously by UBM using a 25-MHz transducer on 3 consecutive days. Both ovaries were clearly distinguished in all 12 rabbits during each examination. Antral follicles ranging from 0.6 to 3.1 mm were detected and the number of follicles ≥0.6 mm per pair of ovaries ranged from 7 to 18. Data were analysed by one-way ANOVA. The number and diameter of follicles ≥0.6 mm per rabbit did not differ among the 3 examinations (mean ± s.e.m.; 11.0 ± 0.82, 11.8 ± 0.85 and 12.3 ± 0.75 follicles and 1.3 ± 0.07, 1.3 ± 0.07 and 1.4 ± 0.07 mm, respectively). However, follicles ≥2 mm in diameter were detected in 3 out of 12 rabbits on the first and second days and in 5 out of 12 rabbits on the third day. One or more corpora lutea were detected in the same 2 rabbits (2/12) during each of the 3 examination days. Two months after surgery, ovarian structures were clearly discernible by UBM in 11 of 24 translocated ovaries. Image deterioration was attributed to the attenuation of sound waves associated with increased skin thickness and growth of subcutaneous fat and connective tissue around the ovary, assessed at necropsy. In summary, translocated ovaries continued to function in their new location and transcutaneous UBM permitted serial visualisation of ovarian structures in live rabbits. Therefore, this new approach provides a nonterminal alternative for repetitive examination of rabbit ovaries and is particularly appropriate for experiments involving factors associated with ovulation induction in rabbits. This research was supported by the Natural Sciences and Engineering Research Council of Canada.

scholarly journals Сell-free D N A in follicular fluid of women with different parameters of ovarian function

2019 ◽  
Vol 18 (2) ◽  
pp. 16-23
Author(s):  
E. A. Andreeva ◽  
N. A. Khonina ◽  
E. N. Demchenko ◽  
E. D. Gavrilova ◽  
N. M. Pasman ◽  
...  
Keyword(s):  
Follicular Fluid ◽  
Ovulation Induction ◽  
Ovarian Function ◽  
Natural Cycle ◽  
Hormonal Stimulation ◽  
Ovulation Stimulation ◽  
Natural Cycle Ivf ◽  
Oocyte Donors ◽  
The Relationship

The aim of the study was to evaluate cell-free DNA (cfDNA) in the follicular fluid (FF) of women undergoing IVF treatment and to analyze the relationship between cfDNA levels and the parameters of folliculogenesis and oogenesis as well as the quality of embryos. Materials and methods. The study included 53 women aged 20 to 45 years. In 49 patients, oocytes were obtained by stimulating ovulation with gonadotropins, and 4 patients underwent natural cycle IVF without hormonal stimulation. Measurement of cfDNA was carried out by fluorimetry using QuantiFluor™ Handheld Fluorometers (BioSilica, Russian Federation). Results. The FF of women with ovulation stimulation revealed a higher level of cfDNA as opposed to FF of women in the natural cycle. There were no differences in the cfDNA levels in women with infertility and oocyte donors. Women with infertility lasting for more than 5 years had a higher level of cfDNA. Women with the elevated anti-Mullerian hormone (AMH) levels were characterized by the high FF cfDNA concentration and a large number of follicles. Likewise, correlation analysis showed that FF cfDNA was significantly and positively correlated with the AMH level. The obtained data revealed the participation of cfDNA in different stages of oogenesis. Conclusions. The level of FF cfDNA in women may serve as an additional biomarker of the effectiveness of ovulation induction.

OVARIAN FUNCTION AND OVULATION INDUCTION

The Lancet ◽  
1966 ◽  
Vol 288 (7471) ◽  
pp. 1030 ◽  
Author(s):  
A.C. Crooke ◽  
W.R. Butt ◽  
P.V. Bertrand
Keyword(s):  
Ovulation Induction ◽  
Ovarian Function

Factors influencing adhesive bonding at the bone/implant interface

1992 ◽  
Vol 50 (2) ◽  
pp. 1114-1115
Author(s):  
Julie A. Martini ◽  
Robert H. Doremus
Keyword(s):  
Electron Microscopy ◽  
Adhesive Bonding ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Bone Implant ◽  
Factors Influencing ◽  
Lr White ◽  
Transmission Electron ◽  
New Zealand White Rabbits ◽  
Scanning Electron

Tracy and Doremus have demonstrated chemical bonding between bone and hydroxylapatite with transmission electron microscopy. Now researchers ponder how to improve upon this bond in turn improving the life expectancy and biocompatibility of implantable orthopedic devices.This report focuses on a study of the- chemical influences on the interfacial integrity and strength. Pure hydroxylapatite (HAP), magnesium doped HAP, strontium doped HAP, bioglass and medical grade titanium cylinders were implanted into the tibial cortices of New Zealand white rabbits. After 12 weeks, the implants were retrieved for a scanning electron microscopy study coupled with energy dispersive spectroscopy.Following sacrifice and careful retrieval, the samples were dehydrated through a graduated series starting with 50% ethanol and continuing through 60, 70, 80, 90, 95, and 100% ethanol over a period of two days. The samples were embedded in LR White. Again a graduated series was used with solutions of 50, 75 and 100% LR White diluted in ethanol.

Factors Influencing Electrophysiologic Responsivity in Normal Adults

1965 ◽  
Vol 8 (4) ◽  
pp. 323-347
Author(s):  
Robert Goldstein ◽  
Benjamin RosenblÜt
Keyword(s):  
Conditioned Stimulus ◽  
White Light ◽  
Pure Tone ◽  
Alpha Rhythm ◽  
White Women ◽  
White Men ◽  
Inverse Relation ◽  
Factors Influencing ◽  
Electrodermal Responses ◽  
Normal Adults

Electrodermal and electroencephalic responsivity to sound and to light was studied in 96 normal-hearing adults in three separate sessions. The subjects were subdivided into equal groups of white men, white women, colored men, and colored women. A 1 000 cps pure tone was the conditioned stimulus in two sessions and white light was used in a third session. Heat was the unconditioned stimulus in all sessions. Previously, an inverse relation had been found in white men between the prominence of alpha rhythm in the EEG and the ease with which electrodermal responses could be elicited. This relation did not hold true for white women. The main purpose of the present study was to answer the following questions: (1) are the previous findings on white subjects applicable to colored subjects? (2) are subjects who are most (or least) responsive electrophysiologically on one day equally responsive (or unresponsive) on another day? and (3) are subjects who are most (or least) responsive to sound equally responsive (or unresponsive) to light? In general, each question was answered affirmatively. Other factors influencing responsivity were also studied.

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