SVTPR SOFTWARE VALIDATION TEST PROCEDURES

2002 ◽  
pp. 651-664
Keyword(s):  
Software Validation ◽  
Test Procedures ◽  
Validation Test

scholarly journals Evaluation of a Lyophilized CRISPR-Cas12 Assay for a Sensitive, Specific, and Rapid Detection of SARS-CoV-2

Viruses ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 420
Author(s):  
Lucía Ana Curti ◽  
Ivana Primost ◽  
Sofia Valla ◽  
Daiana Ibañez Alegre ◽  
Cecilia Olguin Perglione ◽  
...  
Keyword(s):  
Respiratory Pathogens ◽  
Kappa Index ◽  
Test Procedures ◽  
Validation Test ◽  
Initial Validation ◽  
Highly Sensitive ◽  
Wide Range ◽  
Sophisticated Equipment ◽  
Lysis Buffer ◽  
Threshold Range

We evaluated a lyophilized CRISPR-Cas12 assay for SARS-CoV-2 detection (Lyo-CRISPR SARS-CoV-2 kit) based on reverse transcription, isothermal amplification, and CRISPR-Cas12 reaction. From a total of 210 RNA samples extracted from nasopharyngeal swabs using spin columns, the Lyo-CRISPR SARS-CoV-2 kit detected 105/105 (100%; 95% confidence interval (CI): 96.55–100) positive samples and 104/105 (99.05%; 95% CI: 94.81–99.97) negative samples that were previously tested using commercial RT-qPCR. The estimated overall Kappa index was 0.991, reflecting an almost perfect concordance level between the two diagnostic tests. An initial validation test was also performed on 30 nasopharyngeal samples collected in lysis buffer, in which the Lyo-CRISPR SARS-CoV-2 kit detected 20/21 (95.24%; 95% CI: 76.18–99.88) positive samples and 9/9 (100%; 95% CI: 66.37–100) negative samples. The estimated Kappa index was 0.923, indicating a strong concordance between the test procedures. The Lyo-CRISPR SARS-CoV-2 kit was suitable for detecting a wide range of RT-qPCR-positive samples (cycle threshold range: 11.45–36.90) and dilutions of heat-inactivated virus (range: 2.5–100 copies/µL); no cross-reaction was observed with the other respiratory pathogens tested. We demonstrated that the performance of the Lyo-CRISPR SARS-CoV-2 kit was similar to that of commercial RT-qPCR, as the former was highly sensitive and specific, timesaving (1.5 h), inexpensive, and did not require sophisticated equipment. The use of this kit would reduce the time taken for diagnosis and facilitate molecular diagnosis in low-resource laboratories.

scholarly journals SentiHotel: a sentiment analysis application of hotel services using an optimized neural network

2021 ◽  
Vol 10 (3) ◽  
Author(s):  
Dyah Apriliani ◽  
Taufiq Abidin ◽  
Edhy Sutanta ◽  
Amir Hamzah ◽  
Oman Somantri
Keyword(s):  
Neural Network ◽  
Sentiment Analysis ◽  
Software Validation ◽  
Application Development ◽  
Validation Test ◽  
Proposed Model ◽  
Hotel Service ◽  
Analysis Application ◽  
Evaluation Stage ◽  
The Right

An assessed hotel service is necessary for tourists and everyone who is traveling, however currently it is still difficult to find recommended hotel information. The solution provided in this research is to propose a smart application that has been developed by implementing machine learning in it. The purpose is to build a sentiment review smart application by applying the sentiment analysis hybrid model of the best neural network (NN) algorithm model that has been optimized using genetic algorithms. To get the right model, the research method was carried out with experiments starting from the initial stages of conducting data preprocessing, tokenization, weighting, modeling experiments, and conducting the system evaluation stage to determine the success of the proposed model. The progress of the application development system is by using the prototyping model. SentiHotel is a sentiment application that was successfully built to provide a solution for tourists in assessing a hotel service. The software validation test is carried out using the blackbox method and the results show that the SentHotel application is in accordance with the expected result; all system functions can run properly.

General considerations for skin test procedures in the diagnosis of drug hypersensitivity

Allergy ◽  
2002 ◽  
Vol 57 (1) ◽  
pp. 45-51 ◽  
Author(s):  
K. Brockow ◽  
A. Romano ◽  
M. Blanca ◽  
J. Ring ◽  
W. Pichler ◽  
...  
Keyword(s):  
Skin Test ◽  
Drug Hypersensitivity ◽  
Test Procedures

Reliability of Laboratory Tests for the Control of Oral Anticoagulation

1974 ◽  
Vol 32 (02/03) ◽  
pp. 483-491
Author(s):  
E. A Loeliger ◽  
M. J Boekhout-Mussert ◽  
L. P van Halem-Visser ◽  
J. D. E Habbema ◽  
H de Jonge
Keyword(s):  
Human Brain ◽  
Laboratory Tests ◽  
Test Procedure ◽  
Rabbit Brain ◽  
Normal Range ◽  
Test Procedures ◽  
Discrimination Power ◽  
Power Of The Test ◽  
Assay Procedure ◽  
Low Sensitivity

SummaryThe present study concerned the reproducibility of the so-called prothrombin time as assessed with a series of more commonly used modifications of the Quick’s onestage assay procedure, i.e. the British comparative reagent, homemade human brain thromboplastin, Simplastin, Simplastin A, and Thrombotest. All five procedures were tested manually on pooled lyophilized normal and patients’ plasmas. In addition, Simplastin A and Thrombotest were investigated semiautomatically on individual freshly prepared patients’ plasmas. From the results obtained, the following conclusions may be drawn :The reproducibility of results obtained with manual reading on lyophilized plasmas is satisfactory for all five test procedures. For Simplastin, the reproducibility of values in the range of insufficient anticoagulation is relatively low due to the low discrimination power of the test procedure in the near-normal range (so-called low sensitivity of rabbit brain thromboplastins). The reproducibility of Thrombotest excels as a consequence of its particularly easily discerned coagulation endpoint.The reproducibility of Thrombotest, when tested on freshly prepared plasmas using Schnitger’s semiautomatic coagulometer (a fibrinometer-liJce apparatus), is no longer superior to that of Simplastin A.The constant of proportionality between the coagulation times formed with Simplastin A and Thrombotest was estimated at 0.64.Reconstituted Thrombotest is stable for 24 hours when stored at 4° C, whereas reconstituted Simplastin A is not.The Simplastin A method and Thrombotest seem to be equally sensitive to “activation” of blood coagulation upon storage.

Use of a Lyophilized Reference Plasma to Compare Coagulation Test Procedures

1975 ◽  
Vol 34 (02) ◽  
pp. 426-444 ◽  
Author(s):  
J Kahan ◽  
I Nohén
Keyword(s):  
Oral Anticoagulant Therapy ◽  
Repeated Measurements ◽  
Test Procedures ◽  
Coagulation Activity ◽  
Close Relationship ◽  
Measured Activity ◽  
Test Materials ◽  
The Difference ◽  
The Stability ◽  
The One

SummaryIn 4 collaborative trials, involving a varying number of hospital laboratories in the Stockholm area, the coagulation activity of different test materials was estimated with the one-stage prothrombin tests routinely used in the laboratories, viz. Normotest, Simplastin-A and Thrombotest. The test materials included different batches of a lyophilized reference plasma, deep-frozen specimens of diluted and undiluted normal plasmas, and fresh and deep-frozen specimens from patients on long-term oral anticoagulant therapy.Although a close relationship was found between different methods, Simplastin-A gave consistently lower values than Normotest, the difference being proportional to the estimated activity. The discrepancy was of about the same magnitude on all the test materials, and was probably due to a divergence between the manufacturers’ procedures used to set “normal percentage activity”, as well as to a varying ratio of measured activity to plasma concentration. The extent of discrepancy may vary with the batch-to-batch variation of thromboplastin reagents.The close agreement between results obtained on different test materials suggests that the investigated reference plasma could be used to calibrate the examined thromboplastin reagents, and to compare the degree of hypocoagulability estimated by the examined PIVKA-insensitive thromboplastin reagents.The assigned coagulation activity of different batches of the reference plasma agreed closely with experimentally obtained values. The stability of supplied batches was satisfactory as judged from the reproducibility of repeated measurements. The variability of test procedures was approximately the same on different test materials.

DEVELOPMENT OF A TEACHING BOOK BASED ON LOCAL WISDOM OF JEPARA DISTRICT FOR CLASS V ELEMENTARY SCHOOL STUDENTSDEVELOPMENT OF A TEACHING BOOK BASED ON LOCAL WISDOM OF JEPARA DISTRICT FOR CLASS V ELEMENTARY SCHOOL STUDENTS

2020 ◽  
Vol 4 (6) ◽  
Author(s):  
Zaenal Arifin
Keyword(s):  
Elementary School ◽  
Data Collection ◽  
Learning Outcomes ◽  
Elementary School Students ◽  
Descriptive Analysis ◽  
School Students ◽  
Initial Product ◽  
Class V ◽  
Validation Test ◽  
Analysis Technique

This study aims to: (1) develop thematic textbooks based on local wisdom of our best friend's environmental theme material for grade V elementary school students, (2) Know the validity, practicality and effectiveness of thematic textbooks based on local wisdom of our best friend's environmental theme material for grade students V elementary school. The development of thematic textbook learning media based on local wisdom uses the Research and Development (R & D) method. The development stages include: (1) Potentials and problems, (2) Data collection, (3) Product design, (4) Design validation, (5) Design revision, (6) Product testing, (7) Product revision (Sugiyono, 2016). The research was conducted with 33 students from class V SDN 2 Sendang Jepara Regency. Initial product development is a process of making media based on needs analysis. Expert testing or validation was carried out by 2 experts, namely media experts and material experts. The data collection method in this research is observation, interview, and questionnaire. The data analysis technique used descriptive analysis and comparison test of two independent groups, namely using the independent t-test. Based on the results of the trial, this thematic teaching material is very valid, very interesting, effective and can be applied in learning. This can be seen from the results of the validation test from three experts which show that the product is good. The results of the effectiveness test showed that the experimental class students had better learning outcomes than the control class. This means that textbooks based on local wisdom of Jepara Regency are developed effectively

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