Wound Healing Assay

A wound healing assay is simple but effective method to study cell migration in vitro. Cell migration in vitro was found to mimic migration in vivo to some extent [1,2]. In wound healing assays, a “wound” is created by either scraping or mechanically crushing cells in a monolayer, thereby forming a denuded area. Cells migrate into the denuded area to complete coverage, and thereby “heal” the wound. Micrographs at regular time intervals are captured during such experiments for analysis of the process of migration.

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Encyclopedia of Cancer ◽

10.1007/978-3-540-47648-1_6262 ◽

2008 ◽

pp. 3211-3211

Keyword(s):

Wound Healing ◽

Wound Healing Assay

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Wound Healing Assay

Encyclopedia of Cancer ◽

10.1007/978-3-642-16483-5_6262 ◽

2011 ◽

pp. 3958-3958

Keyword(s):

Wound Healing ◽

Wound Healing Assay

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Neuropilin 1 modulates TGF-β1-induced epithelial–mesenchymal transition in non-small cell lung cancer

10.21203/rs.2.10348/v1 ◽

2019 ◽

Author(s):

Zongli Ding ◽

Wenwen Du ◽

Zhe Lei ◽

Yang Zhang ◽

Jianjie Zhu ◽

...

Keyword(s):

Lung Cancer ◽

Wound Healing ◽

Epithelial Mesenchymal Transition ◽

Small Cell ◽

Migration And Invasion ◽

Wound Healing Assay ◽

Small Cell Lung ◽

Mesenchymal Transition ◽

Tgf Β1

Abstract Background: TGF-β1 signaling is a potent inducer of epithelial-mesenchymal transition (EMT) in various cancers. Our previous study has indicated that NRP1 was significantly up-regulated and acted as a vital promoter in the metastasis of non-small cell lung cancer (NSCLC). However, the function of NRP1 in regulation of TGF-β1-induced EMT and NSCLC cell migration and invasion remained unclear. Methods: The differential expression level of NRP1 was determined by RT-PCR analysis in human tissue samples with or without lymph node metastasis. Transwell assay and wound healing assay were conducted to determine cell ability of migration. Lentivirus-mediated stable knockdown and overexpression of NRP1 cell lines were constructed. Exogenous TGF-β1 stimulation, SIS3 treatment, western blot analysis and in vivo metastatic model were utilized to clarify the underlying regulatory mechanism. Results: Increased expression of NRP1 was found in metastatic NSCLC tissues and can promote NSCLC metastasis in vivo. Transwell assays, wound healing assay and western blot analysis showed that knockdown of NRP1 significantly inhibited TGF-β1-mediated EMT and migratory and invasive capabilities of A549 and H226 cells. Furthermore, overexpression of NRP1 could weak the decreased migratory and invasive capabilities with SIS3 treatment. Co-IP data showed that NRP1 can interact with TGFβRⅡ to induce EMT. Conclusion: This is the first time to report that NRP1 can modulate TGF-β1-induced EMT and cell migration and invasion in NSCLC.

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Wound Healing Assay for Melanoma Cell Migration

Methods in Molecular Biology - Melanoma ◽

10.1007/978-1-0716-1205-7_4 ◽

2021 ◽

pp. 65-71

Author(s):

Juliano T. Freitas ◽

Ivan Jozic ◽

Barbara Bedogni

Keyword(s):

Wound Healing ◽

Cell Migration ◽

Melanoma Cell ◽

Wound Healing Assay

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CHANGES OF PER2 GENE KNOCKOUT ON TISSUE REPAIR: EPIDERMAL WOUND HEALING ASSAY IN VIVO

Oral Surgery Oral Medicine Oral Pathology and Oral Radiology ◽

10.1016/j.oooo.2020.04.669 ◽

2020 ◽

Vol 130 (3) ◽

pp. e254

Author(s):

VERONICA QUISPE YUJRA ◽

ERICKA JANINE D. DA SILVEIRA ◽

DANIEL ARAKI RIBEIRO ◽

ROGERIO CASTILHOS ◽

CRISTIANE SQUARIZE

Keyword(s):

Wound Healing ◽

Tissue Repair ◽

Gene Knockout ◽

Wound Healing Assay

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Investigating Epidermal Interactions Through an In Vivo Cutaneous Wound-Healing Assay

Methods in Molecular Biology - Wound Regeneration ◽

10.1007/978-1-0716-0845-6_1 ◽

2020 ◽

pp. 1-11

Author(s):

John L. Zemkewicz ◽

Racheal G. Akwii ◽

Constantinos M. Mikelis ◽

Colleen L. Doçi

Keyword(s):

Wound Healing ◽

Cutaneous Wound Healing ◽

Wound Healing Assay ◽

Cutaneous Wound

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