Optochemical Nanosensors for Noninvasive Cellular Analysis

2002 ◽  
Author(s):  
Heather Clark ◽  
Raoul Kopelman ◽  
Susan Barker
Keyword(s):  
Cellular Analysis

The promises and perfidy of cryomicroscopy and analysis

1994 ◽  
Vol 52 ◽  
pp. 382-383
Author(s):  
Patrick Echlin
Keyword(s):  
Low Temperature ◽  
High Energy ◽  
Short Paper ◽  
List Type ◽  
Time Resolved ◽  
Energy Beam ◽  
Cellular Analysis ◽  
Dissolved Ions ◽  
Embedding Medium ◽  
Low Temperature Microscopy

The unusual title of this short paper and its accompanying tutorial is deliberate, because the intent is to investigate the effectiveness of low temperature microscopy and analysis as one of the more significant elements of the less interventionist procedures we can use to prepare, examine and analyse hydrated and organic materials in high energy beam instruments. The promises offered by all these procedures are well rehearsed and the litany of petitions and responses may be enunciated in the following mantra.Vitrified water can form the perfect embedding medium for bio-organic samples.Frozen samples provide an important, but not exclusive, milieu for the in situ sub-cellular analysis of the dissolved ions and electrolytes whose activities are central to living processes.The rapid conversion of liquids to solids provides a means of arresting dynamic processes and permits resolution of the time resolved interactions between water and suspended and dissolved materials.The low temperature environment necessary for cryomicroscopy and analysis, diminish, but alas do not prevent, the deleterious side effects of ionizing radiation.Sample contamination is virtually eliminated.

scholarly journals S2K Guideline for Diagnosis of Idiopathic Pulmonary Fibrosis

Respiration ◽  
2021 ◽  
pp. 1-34
Author(s):  
Jürgen Behr ◽  
Andreas Günther ◽  
Francesco Bonella ◽  
Julien Dinkel ◽  
Ludger Fink ◽  
...  
Keyword(s):  
Idiopathic Pulmonary Fibrosis ◽  
Pulmonary Fibrosis ◽  
Disease Diagnosis ◽  
Diagnostic Process ◽  
High Resolution Computed Tomography ◽  
Cellular Analysis ◽  
Golden Standard ◽  
Technical Advances ◽  
Transbronchial Lung Cryobiopsy ◽  
Effective Diagnosis

Idiopathic pulmonary fibrosis (IPF) is a severe and often fatal disease. Diagnosis of IPF requires considerable expertise and experience. Since the publication of the international IPF guideline in the year 2011 and the update 2018 several studies and technical advances have occurred, which made a new assessment of the diagnostic process mandatory. The goal of this guideline is to foster early, confident, and effective diagnosis of IPF. The guideline focusses on the typical clinical context of an IPF patient and provides tools to exclude known causes of interstitial lung disease including standardized questionnaires, serologic testing, and cellular analysis of bronchoalveolar lavage. High-resolution computed tomography remains crucial in the diagnostic workup. If it is necessary to obtain specimens for histology, transbronchial lung cryobiopsy is the primary approach, while surgical lung biopsy is reserved for patients who are fit for it and in whom a bronchoscopic diagnosis did not provide the information needed. After all, IPF is a diagnosis of exclusion and multidisciplinary discussion remains the golden standard of diagnosis.

Cellular analysis of long-term habituation of the gill-withdrawal reflex of Aplysia californica

Science ◽  
1978 ◽  
Vol 202 (4374) ◽  
pp. 1306-1308 ◽  
Author(s):  
V. Castellucci ◽  
T. Carew ◽  
E. Kandel
Keyword(s):  
Aplysia Californica ◽  
Withdrawal Reflex ◽  
Cellular Analysis

scholarly journals In silico, in vitro and cellular analysis with a kinome-wide inhibitor panel correlates cellular LRRK2 dephosphorylation to inhibitor activity on LRRK2

2014 ◽  
Vol 7 ◽  
Author(s):  
Renée Vancraenenbroeck ◽  
Joren De Raeymaecker ◽  
Evy Lobbestael ◽  
Fangye Gao ◽  
Marc De Maeyer ◽  
...  
Keyword(s):  
In Silico ◽  
Inhibitor Activity ◽  
Cellular Analysis

Creation of a primary breast cancer culture repository from patients with a BMI >30 kg/m2: A Mexican endeavor.

2021 ◽  
Vol 39 (15_suppl) ◽  
pp. e12574-e12574
Author(s):  
Daniela Shveid Gerson ◽  
Alejandro Zentella - Dehesa ◽  
Raquel Gerson Cwilich ◽  
Benigno Rodriguez ◽  
Omar Serrano Villamayor ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Primary Breast Cancer ◽  
Medical Center ◽  
Primary Cultures ◽  
Obese Women ◽  
Cellular Analysis ◽  
Response To Chemotherapy

e12574 Background: Currently there are no primary cultures or cell lines derived from patients with breast cancer and obesity. It has been postulated that breast cancer in obese women behaves differently as it does in non-obese women, as is composed of distinct biological features, as was generated in a different metabolic environment, as well as pertains to a different prognosis and different response to chemotherapy, lower rates of overall survival and a greater probability of recurrence. By creating a primary breast cancer culture bank of breast cancer tumors from women with obesity (BMI > 30kg/m2), we will establish a cell line exclusive to obese women in Mexico, where targeted therapy may be tested and treatment may be individualized depending on the characteristics of the patient. Methods: This study recruited 32 women with breast cancer and a BMI > 30 kg/m2, matched by 6 controls with are non-obese women with breast cancer. Elegibility criteria was determined by women with breast cancer confirmed by pathology, who had not been subjected to prior treatment regarding the neoplasm. The breast cancer removing surgeries and the patients were selected from the ABC Medical Center in Mexico City and all procedures were approved by the research and ethics committee of the hospital in question. Results: Through extensive communication a cooperative protocol was established between the departments of surgery, oncology, pathology and nursing to coordinate efforts and be able to take a 2 – 5 mm sample of the breast tumor removed from the patient. To be able to distinguish cancer cells from non-cancer cells (epithelial cells, fibroblasts, adipocytes) the Hayflick limit was be utilized. Once a primary breast cancer culture was established, 12 million cells will be injected into the subscapular area of athymic, nu-nu mice to be able to monitor tumoral growth in vivo and conduct a subsequent cellular analysis, determining it still pertains to the same characteristics of the tumor from which it was obtained. Conclusions: A primary breast cancer culture repository from patients with a BMI > 30 kg/m2 was established. This is the first primary breast cancer culture for both Mexican and obese women with breast cancer, the first in vitro method of analysis of specific characteristics typical of the Mexican population. Translational research may now be conducted on these new tumoral cultures to create individualized therapy for women with the distinct, aforementioned characteristics.

Multifunctional aggregates for precise cellular analysis

2021 ◽  
Author(s):  
Jing-Jing Hu ◽  
Xiaoqi Dong ◽  
Wenlian Jiang ◽  
Fan Xia ◽  
Xiaoding Lou
Keyword(s):  
Cellular Analysis

scholarly journals Motion Analysis of Live Objects by Super-Resolution Fluorescence Microscopy

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Chunyan Yao ◽  
Jianwei Zhang ◽  
Guang Wu ◽  
Houxiang Zhang
Keyword(s):  
Fluorescence Microscopy ◽  
Motion Analysis ◽  
Cell Cancer ◽  
Super Resolution ◽  
Biological Engineering ◽  
Related Field ◽  
Cellular Analysis ◽  
Detection And Tracking ◽  
Motion Behavior ◽  
Analysis Of Motion

Motion analysis plays an important role in studing activities or behaviors of live objects in medicine, biotechnology, chemistry, physics, spectroscopy, nanotechnology, enzymology, and biological engineering. This paper briefly reviews the developments in this area mostly in the recent three years, especially for cellular analysis in fluorescence microscopy. The topic has received much attention with the increasing demands in biomedical applications. The tasks of motion analysis include detection and tracking of objects, as well as analysis of motion behavior, living activity, events, motion statistics, and so forth. In the last decades, hundreds of papers have been published in this research topic. They cover a wide area, such as investigation of cell, cancer, virus, sperm, microbe, karyogram, and so forth. These contributions are summarized in this review. Developed methods and practical examples are also introduced. The review is useful to people in the related field for easy referral of the state of the art.

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