- Feeding Biology: Advances from Field-Based Observations, Physiological Studies, and Molecular Techniques

Cells function through the complex temporal and spatial interplay of ions, metabolites, macromolecules and macromolecular assemblies. Biochemical approaches allow the investigator to define the components and the solution chemical reactions that might be involved in cellular functions. Static structural methods can yield information concerning the 2- and 3-D organization of known and unknown cellular constituents. Genetic and molecular techniques are powerful approaches that can alter specific functions through the manipulation of gene products and thus identify necessary components and sequences of molecular events. However, full knowledge of the mechanism of particular cell functions will require direct measurement of the interplay of cellular constituents. Therefore, there has been a need to develop methods that can yield chemical and molecular information in time and space in living cells, while allowing the integration of information from biochemical, molecular and genetic approaches at the cellular level.

Download Full-text

Anti-inflammatory, antioxidant, anti-tumor and physiological studies on Levisticum officinale-Koch plant

Planta Medica ◽

10.1055/s-0029-1234623 ◽

2009 ◽

Vol 75 (09) ◽

Cited By ~ 3

Author(s):

SR Abd El-Hamid ◽

YI Abeer ◽

SF Hendawy

Keyword(s):

Physiological Studies ◽

Anti Inflammatory

Download Full-text

Characterisation of Type 2N von Willebrand Disease Using Phenotypic and Molecular Techniques

Thrombosis and Haemostasis ◽

10.1055/s-0038-1650401 ◽

1996 ◽

Vol 75 (06) ◽

pp. 959-964 ◽

Cited By ~ 27

Author(s):

I M Nesbitt ◽

A C Goodeve ◽

A M Guilliatt ◽

M Makris ◽

F E Preston ◽

...

Keyword(s):

Direct Sequencing ◽

Von Willebrand Disease ◽

Molecular Techniques ◽

Haemophilia A ◽

Binding Region ◽

Gene Encoding ◽

Covalently Linked ◽

Von Willebrand ◽

Willebrand Factor

Summaryvon Willebrand factor (vWF) is a multimeric glycoprotein found in plasma non covalently linked to factor VIII (FVIII). Type 2N von Willebrand disease (vWD) is caused by a mutation in the vWF gene that results in vWF with a normal multimeric pattern, but with reduced binding to FVIII.We have utilised methods for the phenotypic and genotypic detection of type 2N vWD. The binding of FVIII to vWF in 69 patients, 36 with type 1 vWD, 32 with mild haemophilia A and one possible haemophilia A carrier with low FVIII levels was studied. Of these, six were found to have reduced binding (five type 1 vWD, one possible haemophilia A carrier), DNA was extracted from these patients and exons 18-23 of the vWF gene encoding the FVIII binding region of vWF were analysed. After direct sequencing and chemical cleavage mismatch detection, a Thr28Met mutation was detected in two unrelated individuals, one of whom appears to be a compound heterozygote for the mutation and a null allele. No mutations were found in the region of the vWF gene encoding the FVIII binding region of vWF in the other four patients

Download Full-text