Bioactive Compounds from Okinawan Marine Cyanobacteria

2012 ◽  
pp. 21-26
Author(s):  
Toshiaki Teruya ◽  
Osamu Ohno ◽  
Kiyotake Suenaga
Keyword(s):  
Bioactive Compounds ◽  
Marine Cyanobacteria

scholarly journals Bioprospecting Sponge-Associated Marine Cyanobacteria to Produce Bioactive Compounds

Toxins ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 73 ◽  
Author(s):  
Despoina Konstantinou ◽  
Eleni Mavrogonatou ◽  
Sevasti-Kiriaki Zervou ◽  
Panagiotis Giannogonas ◽  
Spyros Gkelis
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Bioactive Compounds ◽  
Pathogenic Bacteria ◽  
Human Cancer ◽  
Cancer Cell Lines ◽  
Marine Cyanobacteria ◽  
Human Cancer Cell ◽  
Metabolic Potential ◽  
Human Cancer Cell Lines

Marine cyanobacteria are considered a prolific source of bioactive natural products with a range of biotechnological and pharmacological applications. However, data on the production of natural compounds from sponge-associated cyanobacteria are scarce. This study aimed to assess the potential of sponge-associated cyanobacteria strains representing different taxonomic groups for the production of bioactive compounds and the biological activity of their extracts. Phylogenetic analysis of sponge-associated cyanobacteria and screening for the presence of genes encoding non-ribosomal peptide synthetases (NRPSs) and polyketide synthases (PKSs) were performed. Methanol extracts of the sponge-associated strains were analyzed for cyanotoxin production and tested for antioxidant activity and cytotoxic activity against several human cancer cell lines and pathogenic bacteria. PKS were detected in all sponge-associated strains examined, indicating the metabolic potential of the isolates. PKS genes were more ubiquitous than NRPS genes. Cyanotoxins (i.e., cylindrospermopsin, anatoxin-a, nodularin, and microcystins) were not detected in any of the sponge-associated cyanobacterial strains. Strains belonging to Leptothoe, Pseudanabaena, and Synechococcus were found to have activity mainly against Staphylococcus aureus. In addition, sponge-associated Leptothoe strains (TAU-MAC 0915, 1015, 1115, and 1215) were found to be highly cytotoxic and in most cases more effective against human cancer cell lines than against normal cells. Extracts with the most promising bioactivity deserve further investigation in order to isolate and identify the bioactive molecule(s).

scholarly journals Application of Bioactive Thermal Proteome Profiling to Decipher the Mechanism of Action of the Lipid Lowering 132-Hydroxy-pheophytin Isolated from a Marine Cyanobacteria

Marine Drugs ◽  
2019 ◽  
Vol 17 (6) ◽  
pp. 371 ◽  
Author(s):  
Ana Carrasco del Amor ◽  
Sara Freitas ◽  
Ralph Urbatzka ◽  
Olatz Fresnedo ◽  
Susana Cristobal
Keyword(s):  
Bioactive Compounds ◽  
Molecular Mechanisms ◽  
Functional Characterization ◽  
Bioactive Compound ◽  
Lipid Lowering ◽  
Marine Cyanobacteria ◽  
Protein Targets ◽  
Pheophytin A ◽  
Proteome Profiling ◽  
Obesity And Diabetes

The acceleration of the process of understanding the pharmacological application of new marine bioactive compounds requires identifying the compound protein targets leading the molecular mechanisms in a living cell. The thermal proteome profiling (TPP) methodology does not fulfill the requirements for its application to any bioactive compound lacking chemical and functional characterization. Here, we present a modified method that we called bTPP for bioactive thermal proteome profiling that guarantees target specificity from a soluble subproteome. We showed that the precipitation of the microsomal fraction before the thermal shift assay is crucial to accurately calculate the melting points of the protein targets. As a probe of concept, the protein targets of 132-hydroxy-pheophytin, a compound previously isolated from a marine cyanobacteria for its lipid reducing activity, were analyzed on the hepatic cell line HepG2. Our improved method identified 9 protein targets out of 2500 proteins, including 3 targets (isocitrate dehydrogenase, aldehyde dehydrogenase, phosphoserine aminotransferase) that could be related to obesity and diabetes, as they are involved in the regulation of insulin sensitivity and energy metabolism. This study demonstrated that the bTPP method can accelerate the field of biodiscovery, revealing protein targets involved in mechanisms of action (MOA) connected with future applications of bioactive compounds.

scholarly journals Exploring Marine Cyanobacteria for Lead Compounds of Pharmaceutical Importance

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Bushra Uzair ◽  
Sobia Tabassum ◽  
Madiha Rasheed ◽  
Saima Firdous Rehman
Keyword(s):  
Bioactive Compounds ◽  
Biological Activities ◽  
Structural Diversity ◽  
Marine Cyanobacteria ◽  
Pharmacological Activities ◽  
Lead Compounds ◽  
Acquired Immunodeficiency ◽  
Living Organisms ◽  
Immunodeficiency Syndrome ◽  
Excellent Source

The Ocean, which is called the “mother of origin of life,” is also the source of structurally unique natural products that are mainly accumulated in living organisms. Cyanobacteria are photosynthetic prokaryotes used as food by humans. They are excellent source of vitamins and proteins vital for life. Several of these compounds show pharmacological activities and are helpful for the invention and discovery of bioactive compounds, primarily for deadly diseases like cancer, acquired immunodeficiency syndrome (AIDS), arthritis, and so forth, while other compounds have been developed as analgesics or to treat inflammation, and so forth. They produce a large variety of bioactive compounds, including substances with anticancer and antiviral activity, UV protectants, specific inhibitors of enzymes, and potent hepatotoxins and neurotoxins. Many cyanobacteria produce compounds with potent biological activities. This paper aims to showcase the structural diversity of marine cyanobacterial secondary metabolites with a comprehensive coverage of alkaloids and other applications of cyanobacteria.

Investigation of bioactive compounds in the genus Garcinia (Guttiferae) of Cameroon

Planta Medica ◽  
2009 ◽  
Vol 75 (09) ◽  
Author(s):  
B Biloa Messi ◽  
R Ho ◽  
A Meli Lannang ◽  
JG Tangmouo ◽  
A Marston ◽  
...  
Keyword(s):  
Bioactive Compounds

Strategies and Methods for a Sustainable Search for Bioactive Compounds

Planta Medica ◽  
2012 ◽  
Vol 78 (05) ◽  
Author(s):  
L Bohlin ◽  
C Alsmark ◽  
U Göransson ◽  
M Klum ◽  
C Wedén ◽  
...  
Keyword(s):  
Bioactive Compounds
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