scholarly journals Analysis of the Chloroplast Protein Kinase Stt7 during State Transitions

2016 ◽  
pp. 240-266
Keyword(s):  
Protein Kinase ◽  
State Transitions ◽  
Chloroplast Protein

scholarly journals Analysis of the Chloroplast Protein Kinase Stt7 during State Transitions

PLoS Biology ◽  
2009 ◽  
Vol 7 (3) ◽  
pp. e1000045 ◽  
Author(s):  
Sylvain Lemeille ◽  
Adrian Willig ◽  
Nathalie Depège-Fargeix ◽  
Christian Delessert ◽  
Roberto Bassi ◽  
...  
Keyword(s):  
Protein Kinase ◽  
State Transitions ◽  
Chloroplast Protein

scholarly journals Plant Physiology, 2021 Functional redox links between lumen thiol oxidoreductase1 and serine/threonine-protein kinase STN7

2021 ◽  
Author(s):  
Jianghao Wu ◽  
Liwei Rong ◽  
Weijun Lin ◽  
Lingxi Kong ◽  
Dengjie Wei ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Disulfide Bonds ◽  
Kinase Activity ◽  
Light Harvesting ◽  
Photosynthetic Electron Transport ◽  
State Transitions ◽  
Light Harvesting Complex ◽  
Light Harvesting Complex Ii

Abstract In response to changing light quantity and quality, photosynthetic organisms perform state transitions, a process which optimizes photosynthetic yield and mitigates photo-damage. The serine/threonine-protein kinase STN7 phosphorylates the light-harvesting complex of photosystem II (PSII; light-harvesting complex II), which then migrates from PSII to photosystem I (PSI), thereby rebalancing the light excitation energy between the photosystems and restoring the redox poise of the photosynthetic electron transport chain. Two conserved cysteines forming intra- or intermolecular disulfide bonds in the lumenal domain (LD) of STN7 are essential for the kinase activity although it is still unknown how activation of the kinase is regulated. In this study, we show lumen thiol oxidoreductase 1 (LTO1) is co-expressed with STN7 in Arabidopsis (Arabidopsis thaliana) and interacts with the LD of STN7 in vitro and in vivo. LTO1 contains thioredoxin (TRX)-like and vitamin K epoxide reductase domains which are related to the disulfide-bond formation system in bacteria. We further show that the TRX-like domain of LTO1 is able to oxidize the conserved lumenal cysteines of STN7 in vitro. In addition, loss of LTO1 affects the kinase activity of STN7 in Arabidopsis. Based on these results, we propose that LTO1 helps to maintain STN7 in an oxidized active state in state 2 through redox interactions between the lumenal cysteines of STN7 and LTO1.

State transitions and light adaptation require chloroplast thylakoid protein kinase STN7

Nature ◽  
2005 ◽  
Vol 433 (7028) ◽  
pp. 892-895 ◽  
Author(s):  
Stéphane Bellafiore ◽  
Frédy Barneche ◽  
Gilles Peltier ◽  
Jean-David Rochaix
Keyword(s):  
Protein Kinase ◽  
Light Adaptation ◽  
State Transitions ◽  
Thylakoid Protein ◽  
Thylakoid Protein Kinase

scholarly journals The Phosphorylation Status of the Chloroplast Protein Kinase STN7 of Arabidopsis Affects Its Turnover

2011 ◽  
Vol 157 (4) ◽  
pp. 2102-2107 ◽  
Author(s):  
Adrian Willig ◽  
Alexey Shapiguzov ◽  
Michel Goldschmidt-Clermont ◽  
Jean-David Rochaix
Keyword(s):  
Protein Kinase ◽  
Chloroplast Protein

scholarly journals Melatonin Regulates Chloroplast Protein Quality Control via a Mitogen-Activated Protein Kinase Signaling Pathway

Antioxidants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 511
Author(s):  
Hyoung Yool Lee ◽  
Kyoungwhan Back
Keyword(s):  
Quality Control ◽  
Protein Kinase ◽  
Protein Quality ◽  
Starch Synthesis ◽  
Protein Quality Control ◽  
Mapk Signaling ◽  
Mitogen Activated Protein Kinase ◽  
Multiple Substrates ◽  
Chloroplast Protein ◽  
Mitogen Activated Protein

Serotonin N-acetyltransferase 1 (SNAT1), the penultimate enzyme for melatonin biosynthesis has shown N-acetyltransferase activity toward multiple substrates, including histones, serotonin, and plastid proteins. Under two different light conditions such as 50 or 100 μmol m−2 s−1, a SNAT1-knockout (snat1) mutant of Arabidopsis thaliana ecotype Columbia (Col-0) exhibited small size phenotypes relative over wild-type (WT) Arabidopsis Col-0. Of note, the small phenotype is stronger when growing at the 50 μmol m−2 s−1, exhibiting a dwarfism phenotype and delayed flowering. The snat1 Arabidopsis Col-0 accumulated less starch than the WT Col-0. Moreover, snat1 exhibited lower Lhcb1, Lhcb4, and RBCL protein levels, compared with the WT Col-0, but no changes in the corresponding transcripts, suggesting the involvement of melatonin in chloroplast protein quality control (CPQC). Accordingly, caseinolytic protease (Clp) and chloroplast heat shock proteins (CpHSPs), two key proteins involved in CPQC, as well as ROS defense were suppressed in snat1. In contrast, exogenous melatonin treatment induced expression of Clp, CpHSP, APX1, and GST, but not other growth-related genes such as DWF4, KS, and IAA1. Finally, the induction of ClpR1, APX1, and GST1 in response to melatonin was inhibited in the mitogen-activated protein kinase (MAPK) knockdown Arabidopsis (mpk3/6), suggesting that melatonin-mediated CPQC was mediated, in part, by the MAPK signaling cascade. These results suggest that melatonin is involved in CPQC, which plays a pivotal role in starch synthesis in plants.

A role for the actin cytoskeleton in the hormonal and growth-factor-mediated activation of protein kinase B

2001 ◽  
Vol 353 (3) ◽  
pp. 735
Author(s):  
K. PEYROLLIER ◽  
E. HAJDUCH ◽  
A. GRAY ◽  
G. J. LITHERLAND ◽  
A. R. PRESCOTT ◽  
...  
Keyword(s):  
Growth Factor ◽  
Protein Kinase ◽  
Actin Cytoskeleton ◽  
Protein Kinase B
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