3. Polymer-modified Gene Delivery Vectors Retargeted with Recombinant Proteins

2012 ◽  
pp. 53-58
Keyword(s):  
Gene Delivery ◽  
Recombinant Proteins ◽  
Gene Delivery Vectors

Recent advances in the analysis full/empty capsid ratio and genome integrity of adeno-associated virus (AAV) gene delivery vectors

2020 ◽  
Vol 20 ◽  
Author(s):  
L. Hajba ◽  
A. Guttman
Keyword(s):  
Gene Delivery ◽  
High Efficiency ◽  
Analytical Techniques ◽  
Viral Gene ◽  
Adeno Associated Virus ◽  
Gene Delivery Vectors ◽  
Empty Capsid ◽  
Purification Methods ◽  
Viral Gene Delivery

: Adeno-associated virus (AAV) is one of the most promising viral gene delivery vectors with long-term gene expression and disease correction featuring high efficiency and excellent safety in human clinical trials. During the production of AAV vectors,there are several quality control (QC)parameters that should be rigorously monitored to comply with clini-cal safety and efficacy. This review gives a short summary of the most frequently used AVV production and purification methods,focusing on the analytical techniques applied to determine the full/empty capsid ratio and the integrity of the encapsidated therapeutic DNA of the products.

pH-sensitive N,N-(dimethyl)-N-alkanamine-N-oxides as gene delivery vectors

2017 ◽  
Vol 71 (9) ◽  
pp. 1739-1748 ◽  
Author(s):  
Gilda Liskayová ◽  
Lukáš Hubčík ◽  
Katarína Šišková ◽  
Ingrid Pauliková ◽  
Dominika Galliková ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Ph Sensitive ◽  
Gene Delivery Vectors

Structure–activity relationship studies of symmetrical cationic bolasomes as non-viral gene vectors

2016 ◽  
Vol 4 (33) ◽  
pp. 5575-5584 ◽  
Author(s):  
Zheng Huang ◽  
Yi-Mei Zhang ◽  
Qian Cheng ◽  
Ji Zhang ◽  
Yan-Hong Liu ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Structure Activity Relationship ◽  
Activity Relationship ◽  
Viral Gene ◽  
Structure Activity ◽  
Gene Delivery Vectors ◽  
Gene Vectors

Bolalipids based on lysine or cyclen headgroups were synthesized and their structure–activity relationship as gene delivery vectors was studied.

Innate immune response induced by gene delivery vectors

2008 ◽  
Vol 354 (1-2) ◽  
pp. 9-15 ◽  
Author(s):  
Haruna Sakurai ◽  
Kenji Kawabata ◽  
Fuminori Sakurai ◽  
Shinsaku Nakagawa ◽  
Hiroyuki Mizuguchi
Keyword(s):  
Immune Response ◽  
Gene Delivery ◽  
Innate Immune Response ◽  
Innate Immune ◽  
Gene Delivery Vectors

scholarly journals Design of Hybrid Lipid/Retroviral-Like Particle Gene Delivery Vectors

2013 ◽  
Vol 10 (5) ◽  
pp. 1725-1735 ◽  
Author(s):  
Rahul K. Keswani ◽  
Ian M. Pozdol ◽  
Daniel W. Pack
Keyword(s):  
Gene Delivery ◽  
Gene Delivery Vectors

scholarly journals Cationic lipids with a cyclen headgroup: synthesis and structure–activity relationship studies as non-viral gene vectors

RSC Advances ◽  
2017 ◽  
Vol 7 (30) ◽  
pp. 18681-18689 ◽  
Author(s):  
De-Chun Chang ◽  
Yi-Mei Zhang ◽  
Ji Zhang ◽  
Yan-Hong Liu ◽  
Xiao-Qi Yu
Keyword(s):  
Gene Delivery ◽  
Structure Activity Relationship ◽  
Cationic Lipids ◽  
Activity Relationship ◽  
Viral Gene ◽  
Structure Activity Relationships ◽  
Structure Activity ◽  
Gene Delivery Vectors ◽  
Gene Vectors ◽  
Viral Gene Delivery

The structure–activity relationships of cyclen-based cationic lipids as non-viral gene delivery vectors were studied and clarified.

Cholesterol derived cationic lipids as potential non-viral gene delivery vectors and their serum compatibility

2016 ◽  
Vol 26 (10) ◽  
pp. 2401-2407 ◽  
Author(s):  
Jia Ju ◽  
Meng-Lei Huan ◽  
Ning Wan ◽  
Yi-Lin Hou ◽  
Xi-Xi Ma ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Cationic Lipids ◽  
Viral Gene ◽  
Gene Delivery Vectors ◽  
Viral Gene Delivery

Biotinylated Cyclen-Contained Cationic Lipids as Non-Viral Gene Delivery Vectors

2013 ◽  
Vol 82 (4) ◽  
pp. 376-383 ◽  
Author(s):  
Qiang Liu ◽  
Wen-Jing Yi ◽  
Yi-Mei Zhang ◽  
Ji Zhang ◽  
Liandi Guo ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Cationic Lipids ◽  
Viral Gene ◽  
Gene Delivery Vectors ◽  
Viral Gene Delivery

RGD peptide-based non-viral gene delivery vectors targeting integrin αvβ3 for cancer therapy

2018 ◽  
Vol 27 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Shuang Fu ◽  
Xiaodong Xu ◽  
Yu Ma ◽  
Shubiao Zhang ◽  
Shufen Zhang
Keyword(s):  
Cancer Therapy ◽  
Gene Delivery ◽  
Integrin Αvβ3 ◽  
Rgd Peptide ◽  
Viral Gene ◽  
Gene Delivery Vectors ◽  
Viral Gene Delivery

scholarly journals Adeno-Associated Virus (AAV) Capsid Stability and Liposome Remodeling During Endo/Lysosomal pH Trafficking

Viruses ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 668 ◽  
Author(s):  
Bridget Lins-Austin ◽  
Saajan Patel ◽  
Mario Mietzsch ◽  
Dewey Brooke ◽  
Antonette Bennett ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Adeno Associated Virus ◽  
Lysosomal Ph ◽  
Negative Stain ◽  
Gene Delivery Vectors ◽  
Successful Infection ◽  
Monogenic Diseases ◽  
The Stability ◽  
Ssdna Viruses ◽  
Capsid Stability

Adeno-associated viruses (AAVs) are small, non-pathogenic ssDNA viruses being used as therapeutic gene delivery vectors for the treatment of a variety of monogenic diseases. An obstacle to successful gene delivery is inefficient capsid trafficking through the endo/lysosomal pathway. This study aimed to characterize the AAV capsid stability and dynamics associated with this process for a select number of AAV serotypes, AAV1, AAV2, AAV5, and AAV8, at pHs representative of the early and late endosome, and the lysosome (6.0, 5.5, and 4.0, respectively). All AAV serotypes displayed thermal melt temperatures that varied with pH. The stability of AAV1, AAV2, and AAV8 increased in response to acidic conditions and then decreased at pH 4.0. In contrast, AAV5 demonstrated a consistent decrease in thermostability in response to acidification. Negative-stain EM visualization of liposomes in the presence of capsids at pH 5.5 or when heat shocked showed induced remodeling consistent with the externalization of the PLA2 domain of VP1u. These observations provide clues to the AAV capsid dynamics that facilitate successful infection. Finally, transduction assays revealed a pH and temperature dependence with low acidity and temperatures > 4 °C as detrimental factors.

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