Increased Expression of Heat Shock Protein 105 in Rat Uterus of Early Pregnancy and Its Significance in Embryo Implantation

2011 ◽  
pp. 48-66
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Early Pregnancy ◽  
Embryo Implantation ◽  
Rat Uterus

scholarly journals Increased expression of heat shock protein 105 in rat uterus of early pregnancy and its significance in embryo implantation

2009 ◽  
Vol 7 (1) ◽  
pp. 23 ◽  
Author(s):  
Jin-Xiang Yuan ◽  
Li-Juan Xiao ◽  
Cui-Ling Lu ◽  
Xue-Sen Zhang ◽  
Tao Liu ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Early Pregnancy ◽  
Embryo Implantation ◽  
Rat Uterus

34 DIFFERENTIALLY EXPRESSED PROTEINS OF EARLY-STAGE PLACENTA DERIVED FROM CLONED CAT EMBRYOS USING PROTEOMICS ANALYSIS

2012 ◽  
Vol 24 (1) ◽  
pp. 129
Author(s):  
J. I. Bang ◽  
D. W. Bae ◽  
Y. S. Kwon ◽  
G. K. Deb ◽  
B. H. Choi ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Early Pregnancy ◽  
Protein Disulfide Isomerase ◽  
Triosephosphate Isomerase ◽  
Early Stage ◽  
Differentially Expressed ◽  
Differentially Expressed Proteins ◽  
Disulfide Isomerase ◽  
Protein Disulfide

We have previously demonstrated that differentially expressed proteins affect abnormal development and function of cloned term placenta. This is associated with cloned fetus morbidity and mortality. We also frequently observed loss of the cloned fetus and failed development during early pregnancy periods. To confirm the pattern of important gene expression in cloned placenta during pre- and post-implantation, we investigated expression pattern of proteins in early stage (21 days) domestic cat placentas of cloned embryo transfer (CEP; n = 2) and artificial insemination (CP; n = 4) derived pregnancy. The differentially expressed proteins were investigated by 2-DE and MALDI-TOF/MS. Twenty-three proteins were up- and down-regulated at least 1.5-fold in the CEP (P < 0.05) compared with the CP. Differentially expressed proteins were analysed using PDQest program and statistically analysed by 1-way ANOVA using the SPSS software. In CEP, 13 proteins were up-regulated, such as 78-kDa glucose-regulated protein (GRP78), annexin A2 (ANXA2), protein DJ-1 (DJ1), adenylate kinase isoenzyme 1 (AK1), protein disulfide-isomerase A3 (PDIA3), heat shock protein β-1 (HSPB1), actin, cytoplasmic 1 (ACTB), serum albumin (ALB), protein disulfide-isomerase A6 (PDIA6), G protein-regulated inducer of neurite outgrowth 1 (GRIN1) and triosephosphate isomerase (TIM). In contrast, 10 proteins were down-regulated, such as vinculin (VCL), triosephosphate isomerase (TIM), heterogeneous nuclear ribonucleoprotein H (hnRNPH), tropomyosin α-4 (TPM4), 60-kDa heat shock protein, mitochondrial (Hsp60), serum albumin (ALB), calumenin (CALU), keratin type 1 (CK1) and prohibitin (PHB). To validate the identified proteins in the CEP compared with the CP, we investigate a peptide sequences using MALDI-TOF/TOF tandem mass spectrometry. The sequence information obtained a high ions score from NCBI and Swiss-Prot databases. In conclusion, we did identify abnormal expression of proteins that might be associated with impaired development of CEP, which may endanger the cloned fetus during early pregnancy. This work was partly supported by the BK21 program and the KOSEF (10525010001-05N2501-00110) and the Next-generation BioGreen21 program (No. PJ007990012011).

scholarly journals Expression of Heat Shock Protein 25 000 in Rat Uterus during Pregnancy and Pseudopregnancy1

1996 ◽  
Vol 54 (6) ◽  
pp. 1326-1335 ◽  
Author(s):  
Daniel R. Ciocca ◽  
Arturo O. Stati ◽  
Mariel A. Fanelli ◽  
Matthias Gaestel
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Rat Uterus

scholarly journals Expression and regulation of stanniocalcin 1 and 2 in rat uterus during embryo implantation and decidualization

Reproduction ◽  
2006 ◽  
Vol 131 (6) ◽  
pp. 1137-1149 ◽  
Author(s):  
Li-Juan Xiao ◽  
Jin-Xiang Yuan ◽  
Xin-Xin Song ◽  
Yin-Chuan Li ◽  
Zhao-Yuan Hu ◽  
...  
Keyword(s):  
Early Pregnancy ◽  
Embryo Implantation ◽  
Normal Pregnancy ◽  
Implantation Site ◽  
Luminal Epithelium ◽  
Rat Uterus ◽  
Delayed Implantation ◽  
Physiological Processes ◽  
Stanniocalcin 2 ◽  
Weak Expression

Stanniocalcin-1 (STC-1) is a recently discovered polypeptide hormone, while stanniocalcin-2 (STC-2) is a subsequently identified homologue of stanniocalcin-1. Although previous studies have shown that both STC-1 and -2 are involved in various physiological processes, such as ion transport, reproduction and development, their expression in the uterus and roles in implantation and early pregnancy are unclear. Here we have investigated the expression and regulation of both STC-1 and STC-2 in rat uterus during early pregnancy under various physiological conditions. We show that only basal levels of STC-1 and STC-2 mRNA were detected in the uterus from day one (D1) to day five (D5) of pregnancy. STC-2 immunostaining was gradually increased in the glandular epithelium from day two (D2), with a peak occurring on D5. High levels of both STC-1 and STC-2 mRNA were observed in the stoma cells at the implantation site on day six (D6) of pregnancy, whereas their immunostaining signals were also significant in the luminal epithelium. Basal levels of both STC-1 and STC-2 mRNA and STC-1 immunostaining were detected in the uterus with delayed implantation. After the delayed implantation was terminated by estrogen treatment, both STC-1 and STC-2 mRNA signals were significantly induced in the stroma underlying the luminal epithelium at the implantation site, and STC-2 immunostaining was also observed in the luminal epithelium surrounding the implanting blastocyst. Embryo transfer experiments further confirmed that STC-1 and STC-2 expression at the implantation sites was induced by the implanting blastocyst. Both STC-1 mRNA and immunostaining were seen in the decidualized cells from day seven (D7) to day nine (D9) of pregnancy. STC-2 mRNA was also found in the whole decidua from D7 to D9 of pregnancy; STC-2 protein, however, was strictly localized to the primary deciduas on D7 and D8, with a weak expression in the whole deciduas on D9. Consistent with the normal pregnancy process, strong STC-1 and STC-2 mRNA signals were detected in the decidualized cells under artificial decidualization, whereas only basal levels of STC-1 mRNA and immunostaining were observed in the control horn. These data suggest, for the first time, that STC-1 together with STC-2 may play important roles in the processes of implantation and decidualization in the rat.

scholarly journals Cross-Reactivity between Chlamydia trachomatis Heat Shock Protein 10 and Early Pregnancy Factor

2003 ◽  
Vol 10 (3) ◽  
pp. 446-450 ◽  
Author(s):  
Fotini Betsou ◽  
Maria José Borrego ◽  
Nicolas Guillaume ◽  
Maria Anjos Catry ◽  
Sandra Romão ◽  
...  
Keyword(s):  
Heat Shock ◽  
Chlamydia Trachomatis ◽  
Heat Shock Protein ◽  
Early Pregnancy ◽  
Genital Tract ◽  
Cross Reactivity ◽  
Genital Tract Infection ◽  
Early Pregnancy Factor ◽  
History Of ◽  
Chaperonin 10

ABSTRACT Chlamydia trachomatis heat shock protein 10 (Chsp10) is associated with chronic genital tract infection with C. trachomatis. Chsp10 is homologous to human chaperonin 10 (Cpn10) and early pregnancy factor (EPF), a form of human Cpn10 that is specifically secreted at the start of pregnancy. We investigated cross-reactions between serum anti-Chsp10 antibodies and anti-EPF antibodies in pregnant and nonpregnant patients. Pregnancy was found to be associated with the presence of anti-EPF antibodies, which are specifically induced in pregnant women with a history of C. trachomatis infection, and with the presence of serum anti-Chsp10 antibodies. We also found that infertility was associated with the presence of anti-Chsp10 and anti-EPF antibodies. The HLA class II haplotype DR8 DQ4 was associated with the presence of anti-Chsp10 antibodies but not of anti-EPF antibodies.

scholarly journals Signal transducer and activator of transcription 3 (Stat3) expression and activation in rat uterus during early pregnancy

Reproduction ◽  
2004 ◽  
Vol 128 (2) ◽  
pp. 197-205 ◽  
Author(s):  
Chun-Bo Teng ◽  
Hong-Lu Diao ◽  
Hong Ma ◽  
Jing Cong ◽  
Hao Yu ◽  
...  
Keyword(s):  
Early Pregnancy ◽  
Stromal Cells ◽  
Embryo Implantation ◽  
Luminal Epithelium ◽  
Signal Transducer ◽  
Uterine Receptivity ◽  
Rat Uterus ◽  
Stat3 Phosphorylation ◽  
Decidual Cells ◽  
Transcription 3

Signal transducer and activator of transcription 3 (Stat3), a member of the Stat family, is specifically activated during mouse embryo implantation. The aim of this study was to investigate the expression, activation and regulation of Stat3 in rat uterus during early pregnancy, pseudopregnancy, delayed implantation and artificial decidualization. Stat3 mRNA was highly expressed in the luminal epithelium on day 5 and in the luminal epithelium and underlying stromal cells at implantation sites on day 6 of pregnancy. There was a strong level of Stat3 protein expression and phosphorylation in the stromal cells near the lumen and in the luminal epithelium on day 5 of pregnancy, which was similar to day 5 of pseudopregnancy. In the afternoon of day 6, the strong level of Stat3 phosphorylation was detected only in the luminal epithelium. Stat3 was highly expressed and activated in the decidual cells from days 7 to 9 of pregnancy and under artificial decidualization in the present study. Our results suggest that the strong level of Stat3 activation in the luminal epithelium and underlying stromal cells during the pre-implantation period may be important for establishing uterine receptivity as in mice, and the high level of Stat3 expression and activation in decidual cells may play a role during decidualization.

scholarly journals Birth defects and anti–heat shock protein 70 antibodies in early pregnancy

2006 ◽  
Vol 11 (1) ◽  
pp. 101 ◽  
Author(s):  
David F. Child ◽  
Peter R. Hudson ◽  
Claire Hunter-Lavin ◽  
Sagarika Mukhergee ◽  
Susnata China ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Birth Defects ◽  
Early Pregnancy ◽  
Heat Shock Protein 70

Glycyrrhizin effects rat hepatocytes via the reduction of heat shock protein 90 expression

2001 ◽  
Vol 120 (5) ◽  
pp. A357-A357
Author(s):  
T YOH ◽  
T NAKASHIMA ◽  
Y SUMIDA ◽  
Y KAKISAKA ◽  
H ISHIKAWA ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 90 ◽  
Rat Hepatocytes

Ammonia aggravates emotional stress-induced gastric mucosal oxidative injury through the cancellation of heat shock protein 70 (HSP70)

2001 ◽  
Vol 120 (5) ◽  
pp. A152-A152
Author(s):  
H SUZUKI ◽  
S NAGAHASHI ◽  
M MIYAZAWA ◽  
M MORI ◽  
H NAGATA ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Emotional Stress ◽  
Heat Shock Protein 70 ◽  
Oxidative Injury
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