Cytochrome P450 2C - a source of EDHF and reactive oxygen species in the porcine coronary artery

Edhf 2000 ◽  
2003 ◽  
pp. 195-200
Keyword(s):  
Reactive Oxygen Species ◽  
Cytochrome P450 ◽  
Coronary Artery ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Porcine Coronary Artery ◽  
Cytochrome P450 2C

scholarly journals HYPOXIC VASORELAXATION IN PORCINE CORONARY ARTERY IS NOT MEDIATED BY REACTIVE OXYGEN SPECIES

2009 ◽  
Vol 23 (S1) ◽  
Author(s):  
Richard J. Paul ◽  
Peggy Sue Bowman ◽  
Anthony J. Parr ◽  
Yukisato Ishida ◽  
Robert L. Wardle ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Coronary Artery ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Porcine Coronary Artery

Hawthorn berry (crataegus songarica) causes endothelium-dependent relaxation of the porcine coronary artery: Role of Estrogen receptors

2021 ◽  
pp. 1-17
Author(s):  
Waqas Younis ◽  
Alamgeer ◽  
Valerie B. Schini-Kerth ◽  
Muhammad Akmal farooq ◽  
Musaad Althobaiti ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Coronary Artery ◽  
Estrogen Receptors ◽  
Intracellular Signaling ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Src Tyrosine Kinase ◽  
Aqueous Fraction ◽  
Porcine Coronary Artery ◽  
Vascular Insufficiency

BACKGROUND: Fruits of Crataegus songarica are commonly used for the treatment of vascular insufficiency and heart problems. OBJECTIVE: Our aim was to determine the effect of C. songarica on vascular tone and to determine the mechanisms underlying the vasorelaxant properties. METHODS: Extracts of C. songarica were tested for vasodilator activity of porcine coronary artery after pre-contraction with the thromboxane mimetic U46619 in the presence or absence of inhibitors of intracellular signaling cascades. Reactive oxygen species were assessed by dihydroethidine staining and the level of eNOS and AKT phosphorylation was measured by immunohistochemical staining. RESULTS: Extracts of C. songarica berries produced endothelium dependent vasorelaxation, with most significant effect induced by aqueous fraction (AS-CS). This vasorelaxant effect of AS-CS was reduced by inhibition of nitric oxide pathways and inhibition of potassium channels. Inhibition of phosphatidylinositol 3- kinase and Src tyrosine kinase, as well as scavenging of reactive oxygen species, produced an attenuation of the relaxation response. Estrogen receptor antagonists (tamoxifen and ICI 182,782) reduced the AS-CS mediated vasorelaxation. AS-CS also stimulated the endothelial formation of ROS and phosphorylation of Akt and eNOS. CONCLUSION: The data indicated that C. songarica produces an endothelium-dependent vasorelaxation, which is partly dependent upon estrogen receptors, and sensitive to inhibition of ROS/Src/PI3K/NO pathways.

scholarly journals The role of reactive oxygen species in the infarct-limiting effect of hypoxic preconditioning

2021 ◽  
Vol 20 (2) ◽  
pp. 87-91
Author(s):  
A. S. Sementsov ◽  
N. V. Naryzhnaya ◽  
M. A. Sirotina ◽  
L. N. Maslov
Keyword(s):  
Reactive Oxygen Species ◽  
Coronary Artery ◽  
Ischemia Reperfusion ◽  
Reactive Oxygen ◽  
Coronary Artery Occlusion ◽  
Artery Occlusion ◽  
Hypoxic Preconditioning ◽  
Male Rats ◽  
Oxygen Species ◽  
Infarct Size Reduction

Introduction. Increased resistance of the heart to ischemia/reperfusion (I/R) is an urgent aim of physiology, pharmacology, and cardiac surgery, since I/R injury of the heart is often the cause of cardiogenic shock and subsequent death of patients in the postoperative period. Materials and methods. The study was carried out in male rats which were subjected to coronary artery occlusion (45 min) and reperfusion (2 h). Before coronary occlusion, early hypoxic preconditioning (HP) was modeled. The rats were subjected to six sessions of hypoxia (8 % O2, 10 min) and reoxygenation (21 % O2, 10 min) 30 min before coronary artery occlusion. The rats were injected with the following drugs: 1,3-dimethylthiourea (DMTM), 2-mercaptopropionyl glycine (2-MPG), deferoxamine. Results. It was found that HP contributes to infarct size reduction by 30 %. Preliminary administration of DMTM, 2-MPG, deferoxamine eliminated the infarct-reducing effect of HP. Conclisuon. The obtained data indicate that reactive oxygen species are involved in the cardioprotective effect of HP.

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