Breast Cancer Detection Using Surface Plasmon Resonance-Based Biosensors

2012 ◽  
pp. 245-263
Keyword(s):  
Breast Cancer ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Cancer Detection ◽  
Plasmon Resonance ◽  
Breast Cancer Detection

Intercoupling surface plasmon resonance and diffusion reflection measurements for real-time cancer detection

2012 ◽  
Vol 6 (2) ◽  
pp. 188-196 ◽  
Author(s):  
Rinat Ankri ◽  
Amihai Meiri ◽  
Shemuel I. Lau ◽  
Menachem Motiei ◽  
Rachela Popovtzer ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Real Time ◽  
Surface Plasmon ◽  
Cancer Detection ◽  
Plasmon Resonance ◽  
Diffusion Reflection ◽  
And Diffusion

scholarly journals Discovery of Orphan Olfactory Receptor 6M1 as a New Anticancer Target in MCF-7 Cells by a Combination of Surface Plasmon Resonance-Based and Cell-Based Systems

Sensors ◽  
2021 ◽  
Vol 21 (10) ◽  
pp. 3468
Author(s):  
Yae-Rim Choi ◽  
Jaewon Shim ◽  
Jae-Ho Park ◽  
Young-Suk Kim ◽  
Min-Jung Kim
Keyword(s):  
Breast Cancer ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Drug Targets ◽  
Olfactory Receptors ◽  
Cancer Cell Line ◽  
Whole Cells ◽  
Anticancer Target ◽  
Mcf 7

Olfactory receptors (ORs) account for 49% of all G protein-coupled receptors (GPCRs), which are important targets for drug discovery, and hence ORs may also be potential drug targets. Various ORs are expressed in breast cancer cells; however, most of them are orphan receptors, and thus, their functions are unknown. Herein, we present an experimental strategy using a surface plasmon resonance (SPR) system and a cell-based assay that allowed the identification of orphan OR6M1 as a new anticancer target in the MCF-7 breast cancer cell line. After the construction of stable OR6M1-expressing cells, the SPR-based screening of 108 chemicals for ligand activity was performed against OR6M1-expressing whole cells (primary screening) or membrane fragments (secondary screening). As a result, anthraquinone (AQ) and rutin were discovered to be new OR6M1 ligands. Based on calcium imaging in OR6M1-expressing Hana3A cells, AQ and rutin were classified as an OR6M1 agonist and antagonist, respectively. Cell viability and live/dead assays showed that AQ induced the death of MCF-7 cells, which was inhibited by rutin. Therefore, OR6M1 may be considered an anticancer target, and AQ may be considered a chemotherapeutic agent. This combined method can be widely used to discover the ligands and functions of other orphan GPCRs.

Surface plasmon resonance immunosensor for ErbB2 breast cancer biomarker determination in human serum and raw cancer cell lysates

2016 ◽  
Vol 905 ◽  
pp. 156-162 ◽  
Author(s):  
Unai Eletxigerra ◽  
Josu Martinez-Perdiguero ◽  
Rodrigo Barderas ◽  
José M. Pingarrón ◽  
Susana Campuzano ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Surface Plasmon Resonance ◽  
Human Serum ◽  
Cancer Cell ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Cancer Biomarker ◽  
Cell Lysates
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