Regulation of C/N metabolism by reversible protein phosphorylation

1997 ◽  
pp. 194-217
Keyword(s):  
Protein Phosphorylation ◽  
N Metabolism ◽  
Reversible Protein Phosphorylation

scholarly journals Analysis of Flagellar Phosphoproteins from Chlamydomonas reinhardtii

2009 ◽  
Vol 8 (7) ◽  
pp. 922-932 ◽  
Author(s):  
Jens Boesger ◽  
Volker Wagner ◽  
Wolfram Weisheit ◽  
Maria Mittag
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Protein Phosphorylation ◽  
Proteome Analysis ◽  
Physiological Status ◽  
Phosphorylation Sites ◽  
Cell Organelles ◽  
Flagellar Proteins ◽  
Reversible Protein Phosphorylation ◽  
Cilia And Flagella

ABSTRACT Cilia and flagella are cell organelles that are highly conserved throughout evolution. For many years, the green biflagellate alga Chlamydomonas reinhardtii has served as a model for examination of the structure and function of its flagella, which are similar to certain mammalian cilia. Proteome analysis revealed the presence of several kinases and protein phosphatases in these organelles. Reversible protein phosphorylation can control ciliary beating, motility, signaling, length, and assembly. Despite the importance of this posttranslational modification, the identities of many ciliary phosphoproteins and knowledge about their in vivo phosphorylation sites are still missing. Here we used immobilized metal affinity chromatography to enrich phosphopeptides from purified flagella and analyzed them by mass spectrometry. One hundred forty-one phosphorylated peptides were identified, belonging to 32 flagellar proteins. Thereby, 126 in vivo phosphorylation sites were determined. The flagellar phosphoproteome includes different structural and motor proteins, kinases, proteins with protein interaction domains, and many proteins whose functions are still unknown. In several cases, a dynamic phosphorylation pattern and clustering of phosphorylation sites were found, indicating a complex physiological status and specific control by reversible protein phosphorylation in the flagellum.

Chemical Approaches to Reversible Protein Phosphorylation

ChemInform ◽  
2003 ◽  
Vol 34 (37) ◽  
Author(s):  
Philip A. Cole ◽  
Aliya D. Courtney ◽  
Kui Shen ◽  
Zhongsen Zhang ◽  
Yingfeng Qiao ◽  
...  
Keyword(s):  
Protein Phosphorylation ◽  
Reversible Protein Phosphorylation

scholarly journals Ca2+/Calmodulin-dependent Protein Kinase II Regulates Tiam1 by Reversible Protein Phosphorylation

1999 ◽  
Vol 274 (18) ◽  
pp. 12753-12758 ◽  
Author(s):  
Ian N. Fleming ◽  
Cassondra M. Elliott ◽  
F. Gregory Buchanan ◽  
C. Peter Downes ◽  
John H. Exton
Keyword(s):  
Protein Kinase ◽  
Protein Phosphorylation ◽  
Dependent Protein Kinase ◽  
Protein Kinase Ii ◽  
Dependent Protein ◽  
Reversible Protein Phosphorylation
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