A.M. Olovnikov When creating an embryo, cells are synthesizing transitory "perichromosomal" DNA-containing organelles necessary for interpreting positional information

2005 ◽  
pp. 475-490
Keyword(s):  
Positional Information ◽  
Embryo Cells

Morphological Examination of a Herpes-type Virus Indigenous for Tree Shrews

1970 ◽  
Vol 28 ◽  
pp. 160-161
Author(s):  
J. P. Brunschwig ◽  
R. M. McCombs ◽  
R. Mirkovic ◽  
M. Benyesh-Melnick
Keyword(s):  
Electron Microscopy ◽  
Soft Tissue ◽  
Chick Embryo ◽  
Soft Tissue Sarcoma ◽  
Cell Cultures ◽  
Tree Shrew ◽  
Type Virus ◽  
Morphological Examination ◽  
Tree Shrews ◽  
Embryo Cells

A new virus, established as a member of the herpesvirus group by electron microscopy, was isolated from spontaneously degenerating cell cultures derived from the kidneys and lungs of two normal tree shrews. The virus was found to replicate best in cells derived from the homologous species. The cells used were a tree shrew cell line, T-23, which was derived from a spontaneous soft tissue sarcoma. The virus did not multiply or did so poorly for a limited number of passages in human, monkey, rodent, rabbit or chick embryo cells. In the T-23 cells, the virus behaved as members of the subgroup B of herpesvirus, in that the virus remained primarily cell associated.

Spontaneous Production of Type C Virus Particles in a Culture Derived from Rat Embryo Cells

1972 ◽  
Vol 30 ◽  
pp. 288-289
Author(s):  
Elizabeth S. Priori ◽  
T. Shigematsu ◽  
B. Myers ◽  
L. Dmochowski
Keyword(s):  
Virus Particle ◽  
Mouse Embryo ◽  
Calf Serum ◽  
Embryo Cell ◽  
Virus Particles ◽  
Extracellular Virus ◽  
Mouse Embryo Cell ◽  
Mature Virus Particle ◽  
Embryo Cells ◽  
Type C

Spontaneous release of type C virus particles in long-term cultures of mouse embryo cells as well as induction of similar particles in mouse embryo cell cultures with IUDR or BUDR have been reported. The presence of type C virus particles in cultures of normal rat embryos has not been reported.NB-1, a culture derived from embryos of a New Zealand Black (NB) rat (rats obtained from Mr. Samuel M. Poiley, N.C.I., Bethesda, Md.) and grown in McCoy's 5A medium supplemented with 20% fetal calf serum was passaged weekly. Extracellular virus particles similar to murine leukemia particles appeared in the 22nd subculture. General appearance of cells in passage 23 is shown in Fig. 1. Two budding figures and one immature type C virus particle may be seen in Fig. 2. The virus particles and budding were present in all further passages examined (currently passage 39). Various stages of budding are shown in Figs. 3a,b,c,d. Appearance of a mature virus particle is shown in Fig. 4.

Application of Transmission Electron Microscope and Scanning Electron Microscope in experimental tumor studies

1990 ◽  
Vol 48 (3) ◽  
pp. 306-307
Author(s):  
Gao Fengming
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Malignant Transformation ◽  
Transmission Electron Microscope ◽  
Experimental Tumor ◽  
Transmission Electron ◽  
Embryo Cells ◽  
Tumor Studies ◽  
Scanning Electron

Transmission electron microscope(TEM) and scanning electron microscope(SEM) were widely used in experimental tumor studies. They are useful for evaluation of cellular transformation in vitro, classification of histological types of tumors and treating effect of tumors. We have obtained some results as follows:1. Studies on the malignant transformation of mammalian cells in vitro. Syrian golden hamster embryo cells(SGHEC) were transformed in vitro by ThO2 and/or ore dust. In a few days after dust added into medium, some dust crystals were phagocytized. Two weeks later, malignant transformation took place. These cells were of different size, nuclear pleomorphism, numerous ribosomes, increasing of microvilli on cell surface with various length and thickness, and blebs and ruffles(Figs. 1,2). Myelomonocytic leukemic transformation of mouse embryo cells(MEC) was induced in vitro by 3H-TdR. Transformed cells were become round from fusiform. The number of mitochondria and endoplasmic reticulum was reduced, ribosomes and nucleoli increased, shape of nuclei irregular, microvilli increased, and blebs and ruffles appeared(Fig. 3).

Proposal and implementation of parking guidance method

Impact ◽  
2019 ◽  
Vol 2019 (10) ◽  
pp. 12-14
Author(s):  
Akira Kawai ◽  
Masahiro Kenmotsu
Keyword(s):  
Traffic Congestion ◽  
Positional Information ◽  
Associate Professor ◽  
Parking Lots ◽  
Gps Technology ◽  
The World ◽  
Car Park ◽  
Free Spaces ◽  
Interest In Research ◽  
Gain Access

Traffic congestion in parking lots is a common phenomenon across the world and larger commercial facilities with multiple parking areas may be particularly affected as many users struggle to gain access to sought-after parking spots close to their destinations. These popular zones often see traffic jams forming as many vehicles arrive within these regions, while less popular areas may remain free from congestion. This creates a very uneven distribution of traffic, with motorists in popular areas becoming trapped and unable to leave bottleneck regions. As a result, the car park management industry has taken an interest in research into parking guidance. Parking guidance has been developed to help improve efficiencies in car parks, guiding drivers to specific spaces using GPS technology to highlight free spaces near their location detailing the most efficient way to get to that spot. Associate Professor Akira Kawai, who is based at Shiga University in Japan, has been working on a KAKEN project that seeks to leverage real-time positional information to help guide drivers to free spaces within parking lots.

Classifying player positions in second-tier Australian football competitions using technical skill indicators

2021 ◽  
pp. 174795412110102
Author(s):  
Adrian J Barake ◽  
Heather Mitchell ◽  
Constantino Stavros ◽  
Mark F Stewart ◽  
Preety Srivastava
Keyword(s):  
Positional Information ◽  
Multinomial Logistic Model ◽  
Sports Science ◽  
Lower Tier ◽  
Australian Football League ◽  
Australian Football ◽  
Second Tier ◽  
Performance Statistics ◽  
Player Performance ◽  
Player Positions

Efficient recruitment to Australia’s most popular professional sporting competition, the Australian Football League (AFL), requires evaluators to assess athlete performances in many lower tier leagues that serve as pathways. These competitions and their games are frequent, widespread, and challenging to track. Therefore, independent, and reliable player performance statistics from these leagues are paramount. This data, however, is only meaningful to recruiters from AFL teams if accurate player positions are known, which was not the case for the competitions from which most players were recruited. This paper explains how this problem was recently solved, demonstrating a process of knowledge translation from academia to industry, that bridged an important gap between sports science, coaching and recruiting. Positional information which is only available from the AFL competition was used to benchmark and develop scientific classification methods using only predictor variables that are also measured in lower tier competitions. Specifically, a Multinomial Logistic model was constructed to allocate players into four primary positions, followed by a Binary Logit model for further refinement. This novel technique of using more complete data from top tier competitions to help fill informational deficiencies in lower leagues could be extended to other sports that face similar issues.

white+ Transgene Insertions Presenting a Dorsal/Ventral Pattern Define a Single Cluster of Homeobox Genes That Is Silenced by the Polycomb-group Proteins in Drosophila melanogaster

Genetics ◽  
1998 ◽  
Vol 149 (1) ◽  
pp. 257-275 ◽  
Author(s):  
Sophie Netter ◽  
Marie-Odile Fauvarque ◽  
Ruth Diez del Corral ◽  
Jean-Maurice Dura ◽  
Dario Coen
Keyword(s):  
Chromatin Structure ◽  
Target Genes ◽  
Position Effect ◽  
Phenotypic Expression ◽  
Positional Information ◽  
Genomic Region ◽  
Polycomb Group ◽  
Position Effect Variegation ◽  
Trithorax Group ◽  
Clear Cut

AbstractWe used the white gene as an enhancer trap and reporter of chromatin structure. We collected white+ transgene insertions presenting a peculiar pigmentation pattern in the eye: white expression is restricted to the dorsal half of the eye, with a clear-cut dorsal/ventral (D/V) border. This D/V pattern is stable and heritable, indicating that phenotypic expression of the white reporter reflects positional information in the developing eye. Localization of these transgenes led us to identify a unique genomic region encompassing 140 kb in 69D1–3 subject to this D/V effect. This region contains at least three closely related homeobox-containing genes that are constituents of the iroquois complex (IRO-C). IRO-C genes are coordinately regulated and implicated in similar developmental processes. Expression of these genes in the eye is regulated by the products of the Polycomb -group (Pc-G) and trithorax-group (trx-G) genes but is not modified by classical modifiers of position-effect variegation. Our results, together with the report of a Pc -G binding site in 69D, suggest that we have identified a novel cluster of target genes for the Pc-G and trx-G products. We thus propose that ventral silencing of the whole IRO-C in the eye occurs at the level of chromatin structure in a manner similar to that of the homeotic gene complexes, perhaps by local compaction of the region into a heterochromatin-like structure involving the Pc-G products.

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