The effect of melatonin and corticosterone on the phagocytic function of BALB/c mice macrophages

2005 ◽  
pp. 29-38
Keyword(s):  
Phagocytic Function

Impaired phagocytic function and increased immune complexes in diabetics with severe microangiopathy

Diabetes ◽  
1982 ◽  
Vol 31 (1) ◽  
pp. 7-11 ◽  
Author(s):  
M. Iavicoli ◽  
U. Di Mario ◽  
P. Pozzilli ◽  
J. Canalese ◽  
L. Ventriglia ◽  
...  
Keyword(s):  
Immune Complexes ◽  
Phagocytic Function

scholarly journals The inflammatory response induced by Pseudomonas aeruginosa in macrophages enhances apoptotic cell removal

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Adriana Valeria Jäger ◽  
Paula Arias ◽  
Maria Virginia Tribulatti ◽  
Marcela Adriana Brocco ◽  
Maria Victoria Pepe ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Pseudomonas Aeruginosa ◽  
Inflammatory Response ◽  
Apoptotic Cell ◽  
Bacterial Pathogen ◽  
Environmental Cues ◽  
Apoptotic Cells ◽  
Phagocytic Function ◽  
Inflammatory Stimulus ◽  
Inflammatory Conditions

AbstractPathogens phagocytosis and the uptake of apoptotic cells (efferocytosis) are essential macrophages tasks, classically considered as mutually exclusive. Macrophages have been observed to polarize into either pro-inflammatory/microbicidal or anti-inflammatory/efferocytic phenotypes. However, macrophage functions have shown to be more complex. Furthermore, little is known about the regulation of efferocytosis under inflammatory conditions. In this study, we elucidate the modulation of the macrophage efferocytic function during an inflammatory stimulus. We find that bone marrow-derived macrophages (BMDM) are very efficient in engulfing both the bacterial pathogen Pseudomonas aeruginosa and apoptotic cells. BMDM showed a high bactericidal capacity unaffected by the concomitant presence of apoptotic material. Plasticity in macrophage programming, in response to changing environmental cues, may modulate efferocytic capability. In this work, we further show that, after phagocyting and processing Pseudomonas aeruginosa, macrophages highly increase their efferocytic capacity without affecting their phagocytic function. Moreover, we demonstrate that Pseudomonas aeruginosa enhances efferocytosis of these phagocytes through the IL-6 signaling pathway. Our results show that the inflammatory response generated by the bacterial processing enhances these macrophages’ capacity to control inflammation through an increased efferocytosis.

Sodium molybdate improves the phagocytic function in alloxan-induced diabetic rats

2003 ◽  
Vol 145 (2) ◽  
pp. 159-163 ◽  
Author(s):  
Saraswathi Panneerselvam ◽  
Swaminathan Govindasamy
Keyword(s):  
Sodium Molybdate ◽  
Diabetic Rats ◽  
Phagocytic Function

PHAGOCYTIC FUNCTION OF ALVEOLAR MACROPHAGES DURING OPEN HEART SURGERY

1994 ◽  
Vol 81 (SUPPLEMENT) ◽  
pp. A245 ◽  
Author(s):  
N. Kotani ◽  
H. Hashimoto ◽  
J. S. Wang ◽  
C. Y. Lin ◽  
M. F. Roizen
Keyword(s):  
Alveolar Macrophages ◽  
Heart Surgery ◽  
Open Heart Surgery ◽  
Open Heart ◽  
Phagocytic Function

Abstract WP98: Role of Hydrogen Peroxide in Preconditioning of Microglia to Achieve Improved Hematoma Cleanup After ICH

Stroke ◽  
2017 ◽  
Vol 48 (suppl_1) ◽  
Author(s):  
Xiurong Zhao ◽  
Guanghua Sun ◽  
Shun-Ming Ting ◽  
Jaroslaw Aronowski
Keyword(s):  
Oxidative Injury ◽  
Repair Process ◽  
Brain Parenchyma ◽  
Secondary Brain Injury ◽  
Related Factor ◽  
Blood Components ◽  
Phagocytic Function ◽  
Phagocytic Capacity ◽  
Biologically Relevant

Background: Red blood cells ( RBC ) and other blood components deposited in brain parenchyma during intracerebral hemorrhage ( ICH ) are the source of secondary brain injury, inflammation, and oxidative stress. Therefore, a fast and efficient removal of the blood from the brain is essential for ameliorating secondary injury and for recovery and repair process. Microglia/macrophages ( MΦ )-mediated phagocytosis is a key component of hematoma clearance after ICH. However, the high levels of pro-oxidative molecules (including H 2 O 2 ) generated by MΦ during phagocytosis could be highly cytotoxic not only to brain cells, but also to MΦ themselves. Thus, an efficient coupling between MΦ-mediated phagocytosis and anti-oxidative processes is essential for the safe and efficient hematoma cleanup. Methods and Results: H 2 O 2 at higher concentrations is known to be neurotoxic. In this study, we established that H 2 O 2 at submicromolar, biologically relevant concentrations, acts as a pro-survival factor for MΦ. By activating transcription factor nuclear factor-erythroid 2 p45-related factor 2 ( Nrf2 ), a master regulator of anti-oxidative regulation, H 2 O 2 stimulates the expression of many antioxidant proteins, which protects MΦ from oxidative injury and damaging components of inflammation. We established that Nrf2-deficient MΦ are more susceptible to H 2 O 2 -mediated or toxic blood components-mediated damage (“ICH-like” injury). Nrf2-KO mice subjected to ICH experienced more severe brain edema and delayed hematoma resolution. In addition, the phagocytosis of RBCs (in vitro model of hematoma clearance) was significantly diminished in MΦ treated with Nrf2 decoy or in MΦ that were harvested from Nrf2-KO mice. On the other hand, pharmacologic activation of Nrf2 or administration of low dosages of H 2 O 2 improved phagocytic capacity of MΦ toward RBC. Furthermore, low levels of H 2 O 2 protected MΦ from ICH-like injury, suggesting that improved phagocytic function could involve preservation of MΦ’ integrity by activating Nrf2. Conclusion: H 2 O 2 at low doses could protect MΦ for oxidative injury and facilitate their phagocytic function, which could benefit hematoma clearance, inflammation resolution, and improve neurological recovery after ICH.

scholarly journals Assessing Retinal Microglial Phagocytic Function In Vivo Using a Flow Cytometry-based Assay

10.3791/54677 ◽  
2016 ◽  
Author(s):  
Salome Murinello ◽  
Stacey K. Moreno ◽  
Matthew S. Macauley ◽  
Susumu Sakimoto ◽  
Peter D. Westenskow ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Phagocytic Function
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