Health Risks of Nonionizing Radiation and Their Prevention and Mitigation

Calicivirus Inactivation by Nonionizing (253.7-Nanometer-Wavelength [UV]) and Ionizing (Gamma) Radiation

Applied and Environmental Microbiology ◽

10.1128/aem.70.9.5089-5093.2004 ◽

2004 ◽

Vol 70 (9) ◽

pp. 5089-5093 ◽

Cited By ~ 83

Author(s):

Ana Maria de Roda Husman ◽

Paul Bijkerk ◽

Willemijn Lodder ◽

Harold van den Berg ◽

Walter Pribil ◽

...

Keyword(s):

Gamma Radiation ◽

Protein Content ◽

Health Risks ◽

Feline Calicivirus ◽

Culture Methods ◽

Low Protein ◽

Bacteriophage Ms2 ◽

Nonionizing Radiation ◽

Radiation Processes ◽

Adequate Management

ABSTRACT Noroviruses (previously Norwalk-like viruses) are the most common viral agents associated with food- and waterborne outbreaks of gastroenteritis. In the absence of culture methods for noroviruses, animal caliciviruses were used as model viruses to study inactivation by nonionizing (253.7-nm-wavelength [UV]) and ionizing (gamma) radiation. Here, we studied the respiratory feline calicivirus (FeCV) and the presumed enteric canine calicivirus (CaCV) and compared them with the well-studied bacteriophage MS2. When UV irradiation was used, a 3-log10 reduction was observed at a fluence of 120 J/m2 in the FeCV suspension and at a fluence of 200 J/m2 for CaCV; for the more resistant phage MS2 there was a 3-log10 reduction at a fluence of 650 J/m2. Few or no differences were observed between levels of UV inactivation in high- and low-protein-content virus stocks. In contrast, ionizing radiation could readily inactivate MS2 in water, and there was a 3-log10 reduction at a dose of 100 Gy, although this did not occur when the phage was diluted in high-protein-content stocks of CaCV or FeCV. The low-protein-content stocks showed 3-log10 reductions at a dose of 500 Gy for FeCV and at a dose of 300 for CaCV. The inactivation rates for both caliciviruses with ionizing and nonionizing radiation were comparable but different from the inactivation rates for MS2. Although most FeCV and CaCV characteristics, such as overall particle and genome size and structure, are similar, the capsid sequences differ significantly, making it difficult to predict human norovirus inactivation. Adequate management of UV and gamma radiation processes for virus inactivation should limit public health risks.

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