Characterizing RNA–Ligand Interactions Using Two-Dimensional Combinatorial Screening

2016 ◽  
pp. 216-241
Keyword(s):  
Two Dimensional ◽  
Combinatorial Screening ◽  
Ligand Interactions

scholarly journals Two-Dimensional Combinatorial Screening Identifies Specific 6′-Acylated Kanamycin A− and 6′-Acylated Neamine−RNA Hairpin Interactions†

Biochemistry ◽  
2008 ◽  
Vol 47 (48) ◽  
pp. 12670-12679 ◽  
Author(s):  
Olga Aminova ◽  
Dustin J. Paul ◽  
Jessica L. Childs-Disney ◽  
Matthew D. Disney
Keyword(s):  
Two Dimensional ◽  
Combinatorial Screening ◽  
Rna Hairpin

scholarly journals Probing a 2-Aminobenzimidazole Library for Binding to RNA Internal Loops via Two-Dimensional Combinatorial Screening

2012 ◽  
Vol 7 (11) ◽  
pp. 1902-1909 ◽  
Author(s):  
Sai Pradeep Velagapudi ◽  
Alexei Pushechnikov ◽  
Lucas P. Labuda ◽  
Jonathan M. French ◽  
Matthew D. Disney
Keyword(s):  
Two Dimensional ◽  
Combinatorial Screening ◽  
Internal Loops

scholarly journals Two-Dimensional NMR Lineshape Analysis

2016 ◽  
Author(s):  
Christopher Andrew Waudby ◽  
Andres Ramos ◽  
Lisa D Cabrita ◽  
John Christodoulou
Keyword(s):  
Software Tool ◽  
Two Dimensional ◽  
Theoretical Frameworks ◽  
Biomolecular Systems ◽  
One Dimensional ◽  
Lineshape Analysis ◽  
Peak Overlap ◽  
Protein Ligand Interactions ◽  
Kinetic Information ◽  
Ligand Interactions

NMR titration experiments are a rich source of structural, mechanistic, thermodynamic and kinetic information on biomolecular interactions, which can be extracted through the quantitative analysis of resonance lineshapes. However, applications of such analyses are frequently limited by peak overlap inherent to complex biomolecular systems. Moreover, systematic errors may arise due to the analysis of two-dimensional data using theoretical frameworks developed for one-dimensional experiments. Here we introduce a more accurate and convenient method for the analysis of such data, based on the direct quantum mechanical simulation and fitting of entire two-dimensional experiments, which we implement in a new software tool, TITAN (TITration ANalysis). We expect the approach, which we demonstrate for a variety of protein-protein and protein-ligand interactions, to be particularly useful in providing information on multi-step or multi-component interactions.

Two-Dimensional Self-Assembly of a Porphyrin-Polypyridyl Ruthenium(II) Hybrid on HOPG Surface through Metal-Ligand Interactions

ChemPhysChem ◽  
2010 ◽  
pp. NA-NA ◽  
Author(s):  
Aimei Gao ◽  
Xinrui Miao ◽  
Jie Liu ◽  
Ping Zhao ◽  
Jinwang Huang ◽  
...  
Keyword(s):  
Self Assembly ◽  
Two Dimensional ◽  
Ligand Interactions ◽  
Metal Ligand
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