The Hydrophobic-Subtraction Model of Reversed-Phase Column Selectivity

2012 ◽  
pp. 297-376 ◽  
Author(s):  
Lloyd Snyder ◽  
John Dolan ◽  
Daniel Marchand ◽  
Peter Carr
Keyword(s):  
Reversed Phase ◽  
Column Selectivity ◽  
Phase Column ◽  
Reversed Phase Column

A novel method for characterization and comparison of reversed-phase column selectivity

2014 ◽  
Vol 1361 ◽  
pp. 153-161 ◽  
Author(s):  
Jixia Wang ◽  
Chaoran Wang ◽  
Zhimou Guo ◽  
Xuefang Dong ◽  
Yuansheng Xiao ◽  
...  
Keyword(s):  
Reversed Phase ◽  
Novel Method ◽  
Column Selectivity ◽  
Phase Column ◽  
Reversed Phase Column

Contributions to reversed-phase column selectivity

2015 ◽  
Vol 1395 ◽  
pp. 57-64 ◽  
Author(s):  
P.W. Carr ◽  
J.W. Dolan ◽  
J.G. Dorsey ◽  
L.R. Snyder ◽  
J.J. Kirkland
Keyword(s):  
Reversed Phase ◽  
Column Selectivity ◽  
Phase Column ◽  
Reversed Phase Column

Contributions to reversed-phase column selectivity. II. Cation exchange

2011 ◽  
Vol 1218 (40) ◽  
pp. 7110-7129 ◽  
Author(s):  
D.H. Marchand ◽  
P.W. Carr ◽  
D.V. McCalley ◽  
U.D. Neue ◽  
J.W. Dolan ◽  
...  
Keyword(s):  
Cation Exchange ◽  
Reversed Phase ◽  
Column Selectivity ◽  
Phase Column ◽  
Reversed Phase Column

Contributions to reversed-phase column selectivity. I. Steric interaction

2011 ◽  
Vol 1218 (13) ◽  
pp. 1724-1742 ◽  
Author(s):  
P.W. Carr ◽  
J.W. Dolan ◽  
U.D. Neue ◽  
L.R. Snyder
Keyword(s):  
Reversed Phase ◽  
Steric Interaction ◽  
Column Selectivity ◽  
Phase Column ◽  
Reversed Phase Column

Improved separation of C12-C22 fatty acid phenacyl esters by reversed phase column liquid chromatography

1986 ◽  
Vol 51 (12) ◽  
pp. 2722-2726 ◽  
Author(s):  
Tomáš Haniš ◽  
Miroslav Smrž ◽  
Pavel Klír ◽  
Karel Macek ◽  
Zdeněk Deyl
Keyword(s):  
Volume Concentration ◽  
Gradient Elution ◽  
Reversed Phase ◽  
Isocratic Elution ◽  
Water Gradient ◽  
Phenacyl Esters ◽  
Acetonitrile Concentration ◽  
Phase Column ◽  
Reversed Phase Column ◽  
Critical Pairs

Phenacyl esters of C12-C22 fatty acids were separated on Separon SGX C18 column, using a gradient elution with methanol-acetonitrile-water. The proposed gradient showed better resolution of the critical pairs C18:3-C14:0, C16:1-C20:4, and C16:0-C18:1 than the gradient elution with methanol-water or acetonitrile-water, or than the isocratic elution with methanol-acetonitrile-water. The optimum volume concentration (83%) of the sum of both methanol and acetonitrile was maintained constant for 35 min; in this period the acetonitrile concentration decreased linearly from the initial 42-60% to 0% while the methanol concentration increased from the initial 41-23% to 83% at the same rate. After 35 min the elution was completed with a methanol-water gradient. The whole analysis can be performed within 63 min at a flow rate 1 ml/min.

4-Nitrophenol in 4-nitrophenyl phosphate, a substrate for alkaline phosphatase, as measured by paired-ion high-performance liquid chromatography.

1977 ◽  
Vol 23 (12) ◽  
pp. 2288-2291 ◽  
Author(s):  
P H Culbreth ◽  
I W Duncan ◽  
C A Burtis
Keyword(s):  
Alkaline Phosphatase ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Flow Rate ◽  
Mobile Phase ◽  
High Performance ◽  
Reversed Phase ◽  
Nitrophenyl Phosphate ◽  
Phase Column ◽  
Reversed Phase Column

Abstract We used paired-ion high-performance liquid chromatography to determine the 4-nitrophenol content of 4-nitrophenyl phosphate, a substrate for alkaline phosphatase analysis. This was done on a reversed-phase column with a mobile phase of methanol/water, 45/55 by vol, containing 3 ml of tetrabutylammonium phosphate reagent per 200 ml of solvent. At a flow rate of 1 ml/min, 4-nitrophenol was eluted at 9 min and monitored at 404 nm; 4-nitrophenyl phosphate was eluted at 5 min and could be monitored at 311 nm. Samples of 4-nitrophenyl phosphate obtained from several sources contained 0.3 to 7.8 mole of 4-nitrophenol per mole of 4-nitrophenyl phosphate.

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