Biological Implications of Polymeric Scaffolds for Bone Tissue Engineering Developed via Solid Freeform Fabrication

2012 ◽  
pp. 483-507
Author(s):  
Andrew Yeatts ◽  
John Fisher
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Solid Freeform Fabrication ◽  
Freeform Fabrication ◽  
Polymeric Scaffolds

Solid Freeform Fabrication of Polycaprolactone∕Hydroxyapatite Tissue Scaffolds

2008 ◽  
Vol 130 (2) ◽  
Author(s):  
L. Shor ◽  
S. Güçeri ◽  
M. Gandhi ◽  
X. Wen ◽  
W. Sun
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Structural Integrity ◽  
Solid Freeform Fabrication ◽  
Porous Scaffolds ◽  
Freeform Fabrication ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation

Bone tissue engineering is an emerging field providing viable substitutes for bone regeneration. Freeform fabrication provides an effective process tool to manufacture scaffolds with complex shapes and designed properties. We developed a novel precision extruding deposition (PED) technique to fabricate composite polycaprolactone∕hydroxyapatite (PCL∕HA) scaffolds. 25% concentration by weight of HA was used to reinforce 3D scaffolds. Two groups of scaffolds having 60% and 70% porosities and with pore sizes of 450μm and 750μm respectively, were evaluated for their morphology and compressive properties using scanning electron microscopy and the mechanical testing. In vitro cell-scaffold interaction study was carried out using primary fetal bovine osteoblasts. The cell proliferation and differentiation were evaluated by Alamar Blue assay and alkaline phosphatase activity. Our results suggested that compressive modulus of PCL∕HA scaffold was 84MPa for 60% porous scaffolds and was 76MPa for 70% porous scaffolds. The osteoblasts were able to migrate and proliferate for the cultured time over the scaffolds. Our study demonstrated the viability of the PED process to fabricate PCL scaffolds having necessary mechanical property, structural integrity, controlled pore size, and pore interconnectivity desired for bone tissue engineering.

Mechanical Behavior of Complex 3D Calcium Phosphate Cement Scaffolds Fabricated by Indirect Solid Freeform Fabrication In Vivo

2006 ◽  
Vol 309-311 ◽  
pp. 957-960 ◽  
Author(s):  
Leenaporn Jongpaiboonkit ◽  
C.Y. Lin ◽  
P.H. Krebsbach ◽  
S.J. Hollister ◽  
J.W. Halloran
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Calcium Phosphate ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Calcium Phosphate Cement ◽  
Solid Freeform Fabrication ◽  
Micro Ct ◽  
Freeform Fabrication

Calcium phosphate cement is a bioceramic with potential applications for bone-tissue engineering. In this work, controlled porous calcium phosphate scaffolds with interconnected pores were computationally designed by an image-based approach and fabricated by indirect solid freeform fabrication (ISFF) or ‘lost mold’ technique. Voxel finite-element analysis (FEA) showed that mechanical properties of design and fabricated scaffold can be predicted computationally. Scaffolds were then implanted subcutaneously to demonstrate tissue in-growth. Previously, we showed the ability of porous calcium phosphate cement scaffolds to have sufficiently strong mechanical properties for bone tissue engineering applications. This work shows the image-based FEAs from micro-CT scans in vivo (four- and eight weeks). Extensive new bone apposition was noted with micro-CT technique after four- and eight weeks. FEA models of the original design and scaffolds with newly bone formed were compared.

Chitosan/β-TCP composites scaffolds coated with silk fibroin: a bone tissue engineering approach

2021 ◽  
Vol 17 (1) ◽  
pp. 015003
Author(s):  
Lya Piaia ◽  
Simone S Silva ◽  
Joana M Gomes ◽  
Albina R Franco ◽  
Emanuel M Fernandes ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Silk Fibroin ◽  
Cellular Proliferation ◽  
Mechanical Performance ◽  
Tissue Growth ◽  
Polymeric Scaffolds ◽  
Bioactive Agents

Abstract Bone regeneration and natural repair are long-standing processes that can lead to uneven new tissue growth. By introducing scaffolds that can be autografts and/or allografts, tissue engineering provides new approaches to manage the major burdens involved in this process. Polymeric scaffolds allow the incorporation of bioactive agents that improve their biological and mechanical performance, making them suitable materials for bone regeneration solutions. The present work aimed to create chitosan/beta-tricalcium phosphate-based scaffolds coated with silk fibroin and evaluate their potential for bone tissue engineering. Results showed that the obtained scaffolds have porosities up to 86%, interconnectivity up to 96%, pore sizes in the range of 60–170 μm, and a stiffness ranging from 1 to 2 MPa. Furthermore, when cultured with MC3T3 cells, the scaffolds were able to form apatite crystals after 21 d; and they were able to support cell growth and proliferation up to 14 d of culture. Besides, cellular proliferation was higher on the scaffolds coated with silk. These outcomes further demonstrate that the developed structures are suitable candidates to enhance bone tissue engineering.

Recent progress in 3D-printed polymeric scaffolds for bone tissue engineering

2020 ◽  
pp. 59-81 ◽  
Author(s):  
Pierre P.D. Kondiah ◽  
Yahya E. Choonara ◽  
Pariksha J. Kondiah ◽  
Thashree Marimuthu ◽  
Lisa C. du Toit ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Recent Progress ◽  
Polymeric Scaffolds ◽  
3D Printed

In Situ Bone Tissue Engineering With an Endogenous Stem Cell Mobilizer and Osteoinductive Nanofibrous Polymeric Scaffolds

2017 ◽  
Vol 12 (12) ◽  
pp. 1700062 ◽  
Author(s):  
Jong Seung Lee ◽  
Yoonhee Jin ◽  
Hyun-Ji Park ◽  
Kisuk Yang ◽  
Min Suk Lee ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Polymeric Scaffolds ◽  
Endogenous Stem Cell

Additive manufacturing of wet-spun polymeric scaffolds for bone tissue engineering

2012 ◽  
Vol 14 (6) ◽  
pp. 1115-1127 ◽  
Author(s):  
Dario Puppi ◽  
Carlos Mota ◽  
Matteo Gazzarri ◽  
Dinuccio Dinucci ◽  
Antonio Gloria ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Additive Manufacturing ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Polymeric Scaffolds

Bone Tissue Scaffold Technologies Based on RP Adopted Droplet Assembly

2002 ◽  
Vol 758 ◽  
Author(s):  
Renji Zhang ◽  
Yongnian Yan ◽  
Feng Lin
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue ◽  
Solid Freeform Fabrication ◽  
Deposition Process ◽  
Layer By Layer ◽  
Tissue Engineering Scaffolds ◽  
Molecular Scaffold ◽  
Tissue Scaffold ◽  
Freeform Fabrication ◽  
Assembly Technology

ABSTRACTTissue engineering tries to grow replacement tissues to repair damaged bones. In this paper, the fabrication technology of Multi-nozzle Deposition Manufacturing (MDM) was adopted to fabricate scaffolds of a tissue engineered bone at low temperature. The composite of poly(L-lactic acid) and tri-calcium phosphate (TCP) was chosen to form bone tissue engineering scaffolds. The new computer aided manufacturing process can make porous PLLA/TCP scaffolds. A new surface processing technology of apatite coating on bone tissue engineered scaffolds was also adopted. This digital forming technology was based on rapid prototyping (RP), in which a digital droplets assembly technology was introduced. The MDM technology of 4 nozzles was developed based on the layer-by-layer manufacturing principle of Solid Freeform Fabrication (SFF) in our laboratory. The bone scaffolds made by the multi-nozzle deposition process in the MDM system have good biocompatibility and bone conductive properties as a molecular scaffold for bone morphogenic protein (BMP) in the implantation experiment of repairing segment defects in rabbits' and dogs' radiuses.

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