Protein Adsorption at the Biomaterial–Tissue Interface

2011 ◽  
pp. 139-156
Keyword(s):  
Protein Adsorption ◽  
Tissue Interface

scholarly journals Protein adsorption/desorption dynamics on Ca-enriched titanium surfaces: biological implications

2021 ◽  
Vol 26 (6) ◽  
pp. 715-726
Author(s):  
Francisco Romero-Gavilán ◽  
Andreia Cerqueira ◽  
Eduardo Anitua ◽  
Ricardo Tejero ◽  
Iñaki García-Arnáez ◽  
...  
Keyword(s):  
Immune Responses ◽  
Protein Adsorption ◽  
Calcium Ions ◽  
Inflammatory Responses ◽  
Titanium Implant ◽  
Physicochemical Characteristics ◽  
Biological Responses ◽  
Titanium Surfaces ◽  
Tissue Interface ◽  
Adsorption Desorption

AbstractCalcium ions are used in the development of biomaterials for the promotion of coagulation, bone regeneration, and implant osseointegration. Upon implantation, the time-dependent release of calcium ions from titanium implant surfaces modifies the physicochemical characteristics at the implant–tissue interface and thus, the biological responses. The aim of this study is to examine how the dynamics of protein adsorption on these surfaces change over time. Titanium discs with and without Ca were incubated with human serum for 2 min, 180 min, and 960 min. The layer of proteins attached to the surface was characterised using nLC-MS/MS. The adsorption kinetics was different between materials, revealing an increased adsorption of proteins associated with coagulation and immune responses prior to Ca release. Implant–blood contact experiments confirmed the strong coagulatory effect for Ca surfaces. We employed primary human alveolar osteoblasts and THP-1 monocytes to study the osteogenic and inflammatory responses. In agreement with the proteomic results, Ca-enriched surfaces showed a significant initial inflammation that disappeared once the calcium was released. The distinct protein adsorption/desorption dynamics found in this work demonstrated to be useful to explain the differential biological responses between the titanium and Ca-ion modified implant surfaces. Graphic abstract

Comparison of Total Body Tissue Interface Pressure of Specialized Pressure-Relieving Mattresses

2004 ◽  
Vol 14 (2) ◽  
pp. 14 ◽  
Author(s):  
William L. Hickerson ◽  
George M. Slugocki ◽  
Reuben L. Thaker ◽  
Robert Duncan ◽  
John F. Bishop ◽  
...  
Keyword(s):  
Body Tissue ◽  
Interface Pressure ◽  
Tissue Interface ◽  
Total Body

Effect of Sintering Processes on Surface Properties and Protein Adsorption of Hydroxyapatite Ceramic Particles

2010 ◽  
Vol 25 (7) ◽  
pp. 770-774 ◽  
Author(s):  
Hui-Jie ZHANG ◽  
Xiang-Dong ZHU ◽  
Xin-Long WANG ◽  
Hong-Song FAN ◽  
Xing-Dong ZHANG
Keyword(s):  
Protein Adsorption ◽  
Surface Properties ◽  
Hydroxyapatite Ceramic ◽  
Ceramic Particles ◽  
Sintering Processes

Controlled radical polymerization of hydrophilic and zwitterionic brush-like polymers from silk fibroin surfaces

2020 ◽  
Vol 8 (45) ◽  
pp. 10392-10406
Author(s):  
Danielle L. Heichel ◽  
Ngoc Chau H. Vy ◽  
Shawn P. Ward ◽  
Douglas H. Adamson ◽  
Kelly A. Burke
Keyword(s):  
Protein Adsorption ◽  
Radical Polymerization ◽  
Silk Fibroin ◽  
Cell Attachment ◽  
Controlled Radical Polymerization

Silk fibroin films were modified with zwitterionic and hydrophilic brush-like polymers via surface-initiated ATRP, resulting in surfaces that reduced protein adsorption and cell attachment.

scholarly journals Positive Contrast Imaging of SPIO Nanoparticles

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Chenghong Lin ◽  
Shuhui Cai ◽  
Jianghua Feng
Keyword(s):  
Negative Contrast ◽  
Superparamagnetic Iron Oxide ◽  
Positive Contrast ◽  
Disease Diagnosis ◽  
Cell Labeling ◽  
Contrast Imaging ◽  
Advantages And Disadvantages ◽  
Tissue Interface ◽  
Dark Signal ◽  
Ideal Method

It is advantageous to achieve positive contrast images instead of negative contrast images in superparamagnetic iron-oxide (SPIO) nanoparticles-based MR imaging in order to distinguish the signal surrounding SPIO nanoparticles from the dark signal due to local field inhomogeneity and the artifacts due to tissue interface and background noise, eliminate the inherent defects in the traditional MRI such as partial-volume effects and large void volume for reliable visualization, and increase contrast-to-noise ratio. Many methods generating positive signal with SPIO nanoparticles have been developed in the last decade. This paper provides an overview of current visualization methods and states their advantages and disadvantages. In practice, these techniques have been widely applied to cell labeling and disease diagnosis and monitoring. However, there is still a need for an ideal method to achieve both accuracy and sensitivity.

scholarly journals Influence of calcium ion-modified implant surfaces in protein adsorption and implant integration

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Eduardo Anitua ◽  
Andreia Cerqueira ◽  
Francisco Romero-Gavilán ◽  
Iñaki García-Arnáez ◽  
Cristina Martinez-Ramos ◽  
...  
Keyword(s):  
Protein Adsorption ◽  
Titanium Implant ◽  
Volume Density ◽  
Bone Implant ◽  
The Mean ◽  
Intervention Costs ◽  
Medial Section ◽  
Post Implantation

Abstract Background Calcium (Ca) is a well-known element in bone metabolism and blood coagulation. Here, we investigate the link between the protein adsorption pattern and the in vivo responses of surfaces modified with calcium ions (Ca-ion) as compared to standard titanium implant surfaces (control). We used LC–MS/MS to identify the proteins adhered to the surfaces after incubation with human serum and performed bilateral surgeries in the medial section of the femoral condyles of 18 New Zealand white rabbits to test osseointegration at 2 and 8 weeks post-implantation (n=9). Results Ca-ion surfaces adsorbed 181.42 times more FA10 and 3.85 times less FA12 (p<0.001), which are factors of the common and the intrinsic coagulation pathways respectively. We also detected differences in A1AT, PLMN, FA12, KNG1, HEP2, LYSC, PIP, SAMP, VTNC, SAA4, and CFAH (p<0.01). At 2 and 8 weeks post-implantation, the mean bone implant contact (BIC) with Ca-ion surfaces was respectively 1.52 and 1.25 times higher, and the mean bone volume density (BVD) was respectively 1.35 and 1.13 times higher. Differences were statistically significant for BIC at 2 and 8 weeks and for BVD at 2 weeks (p<0.05). Conclusions The strong thrombogenic protein adsorption pattern at Ca-ion surfaces correlated with significantly higher levels of implant osseointegration. More effective implant surfaces combined with smaller implants enable less invasive surgeries, shorter healing times, and overall lower intervention costs, especially in cases of low quantity or quality of bone.

Design of oriented mesoporous silica films for guiding protein adsorption states

2021 ◽  
Author(s):  
Yadong Chai ◽  
Yuri Maruko ◽  
Zizhen Liu ◽  
Motohiro Tagaya
Keyword(s):  
Mesoporous Silica ◽  
Protein Adsorption ◽  
Silica Films

The highly-oriented cylindrical mesoporous silica films were synthesized on the rubbing-treated polyimide, and effectively adsorbed the mesoscale biomolecules such as proteins and guided to be their anisotropic adsorption shapes.

Protein Adsorption on Surfaces Functionalized with COOH Groups Promotes Anti-inflammatory Macrophage Responses

2021 ◽  
Vol 13 (6) ◽  
pp. 7021-7036
Author(s):  
Emily Buck ◽  
Seunghwan Lee ◽  
Laura S. Stone ◽  
Marta Cerruti
Keyword(s):  
Protein Adsorption ◽  
Anti Inflammatory ◽  
Inflammatory Macrophage
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