Label-Free Biosensors for Biomedical Applications

2017 ◽  
pp. 71-104
Author(s):  
Jeffrey W. Chamberlain ◽  
Daniel M. Ratner
Keyword(s):  
Biomedical Applications ◽  
Label Free

scholarly journals Microparticle Inertial Focusing in an Asymmetric Curved Microchannel

Fluids ◽  
2018 ◽  
Vol 3 (3) ◽  
pp. 57 ◽  
Author(s):  
Arzu Özbey ◽  
Mehrdad Karimzadehkhouei ◽  
Hossein Alijani ◽  
Ali Koşar
Keyword(s):  
Biomedical Applications ◽  
Cost Effective ◽  
Label Free ◽  
Promising Technique ◽  
Flow Rates ◽  
Inertial Focusing ◽  
Micro Total Analysis Systems ◽  
Symmetric Geometry ◽  
Total Analysis ◽  
Different Diameters

Inertial Microfluidics offer a high throughput, label-free, easy to design, and cost-effective solutions, and are a promising technique based on hydrodynamic forces (passive techniques) instead of external ones, which can be employed in the lab-on-a-chip and micro-total-analysis-systems for the focusing, manipulation, and separation of microparticles in chemical and biomedical applications. The current study focuses on the focusing behavior of the microparticles in an asymmetric curvilinear microchannel with curvature angle of 280°. For this purpose, the focusing behavior of the microparticles with three different diameters, representing cells with different sizes in the microchannel, was experimentally studied at flow rates from 400 to 2700 µL/min. In this regard, the width and position of the focusing band are carefully recorded for all of the particles in all of the flow rates. Moreover, the distance between the binary combinations of the microparticles is reported for each flow rate, along with the Reynolds number corresponding to the largest distances. Furthermore, the results of this study are compared with those of the microchannel with the same curvature angle but having a symmetric geometry. The microchannel proposed in this study can be used or further modified for cell separation applications.

Strategies for the Development of Metallic‐Nanoparticle‐Based Label‐Free Biosensors and Their Biomedical Applications

ChemBioChem ◽  
2019 ◽  
Vol 21 (5) ◽  
pp. 576-600 ◽  
Author(s):  
Sandeep Kaushal ◽  
Sitansu Sekhar Nanda ◽  
Shashadhar Samal ◽  
Dong Kee Yi
Keyword(s):  
Biomedical Applications ◽  
Label Free ◽  
Metallic Nanoparticle

Label-free multimodal nonlinear optical microscopy for biomedical applications

2021 ◽  
Vol 129 (21) ◽  
pp. 214901
Author(s):  
Guan-Yu Zhuo ◽  
Spandana K U ◽  
Sindhoora K M ◽  
Yury V. Kistenev ◽  
Fu-Jen Kao ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Biomedical Applications ◽  
Nonlinear Optical ◽  
Label Free ◽  
Nonlinear Optical Microscopy

Label-Free 3D Imaging for Lab-on-Chip Biomedical Applications

CLEO: 2014 ◽  
2014 ◽  
Author(s):  
Lisa Miccio ◽  
Francesco Merola ◽  
Pasquale Memmolo ◽  
Pietro Ferraro
Keyword(s):  
Biomedical Applications ◽  
3D Imaging ◽  
Label Free ◽  
Lab On Chip ◽  
On Chip

scholarly journals Three-Dimensional Self-Assembled Nanocarbon For Theranostics Applications

2021 ◽  
Author(s):  
A K M Rezaul Haque Chowdhury
Keyword(s):  
Hela Cells ◽  
Carbon Nanostructures ◽  
Biomedical Applications ◽  
Carbon Nanomaterials ◽  
Three Dimensional ◽  
Cancer Diagnostics ◽  
Label Free ◽  
Diagnostic Applications ◽  
Therapy And Diagnosis

Carbon nanomaterials have been explored for biomedical applications such as scaffolds in tissue engineering, drug delivery carriers, cancer diagnostics and biological imaging. Due to their possible cytotoxicity and biological inertness, they need biological or chemical functionalization to attain biomedical applications. Current research trends are for the synthesis of biocompatible and self-functionalized nanocarbon with prospective application in therapy and diagnosis. The main objectives of this thesis are to synthesize 3D self-functionalized biocompatible nanocarbon for therapeutic and diagnostic applications. The synthesis of the unique three-dimensional carbon nanostructures has been done with ultrashort femtosecond laser processing mechanism, a versatile yet precise technique for nanoscale material generation. First study deals with the synthesis of 3D nanocarbon network and its biocompatibility assessment. Quantitative and qualitative studies of the fibroblast cell response to this nano-network are performed. The findings from the in-vitro study indicate that the platform possesses excellent biocompatibility and promote cell adhesion and subsequent cell proliferation. In next study, the synthesized nanocarbon network (CNRN) platform that possesses a variation in C-C and C-O bond architecture showed dual functionality i. e. cytophilic to fibroblasts but cytotoxic to HeLa cells. Two distict opposite responses like tissue generation for fibroblasts and apoptosis like function for HeLa was observed after 48-hour of culture. The results have potentials or therapeutic appliations. Third study focuses on the diagnostic applications of the nanocarbon. A unique non-plasmonic SERS based bio-sensing platform using 3D nanocarbon is introduced for in-vitro detection and differentiation of HeLa and fibroblast cells. Time based Raman spectroscopy of these cells seeded on nanocarbon revealed chemical fingerprints of intracellular components like DNA/RNA, protein and lipids. Their spectroscopic differences guide differentiation of each cell. Finally, we have synthesized N-enriched nanocarbon probe through nitrogen incorporation-assisted ionization and demonstrate label free SERS based detection of transient variation of cell chemistry and thereby cancer cell diagnosis with N-enriched 3D nanocarbon probe. The results suggested that the SERS functionality not only reveal the chemical fingerprint of the intracellular components (e. g. protein, DNA, RNA etc.) within a cell but also guide detection of cancerous HeLa cells. The results obtained in this thesis point out multifunctional viability of biocompatible self-functionalized nanocarbons for therapy and diagnosis.

Microparticle Inertial Focusing in an Asymmetric Curved Microchannel

2018 ◽  
Author(s):  
Arzu Özbey ◽  
Mehrdad Karimzadehkhouei ◽  
Hossein Alijani ◽  
Ali Koşar
Keyword(s):  
Reynolds Number ◽  
Biomedical Applications ◽  
Cost Effective ◽  
Reynolds Numbers ◽  
Band Width ◽  
Label Free ◽  
Inertial Focusing ◽  
Micro Total Analysis Systems ◽  
Total Analysis ◽  
Asymmetric Microchannel

Inertial microfluidics offers high throughput, label-free, easy to design, and cost-effective solutions and is a promising technique based on hydrodynamic forces (passive techniques) instead of external ones, which can be employed in lab-on-a-chip and micro-total-analysis-systems for focusing, manipulation, and separation of microparticles in chemical and biomedical applications. The current work, studies the focusing behavior of microparticles in an asymmetric curvilinear microchannel. For this purpose, focusing behavior, including position and band width, of microparticles of diameters of 10, 15 and 20 µm, which served as representatives of different cells, in an asymmetric curvilinear microchannel with curvature angle of 280° was experimentally studied at flow rates from 400 to 2700 µL/min (corresponding to Reynolds numbers between 30 and 205). The results revealed that the largest distance between focusing bands of 20 µm and 10 µm microparticles as well as between focusing bands of 15 µm and 10 µm was obtained at Reynolds number of 121. For the case of microparticles of diameters 20 µm and 15 µm, the largest distance was seen at Reynolds number of 144. The focusing band width became smaller in the asymmetric microchannel so that focusing could be more clearly observed in this configuration.

scholarly journals Surface plasmon resonance: principles, methods and applications in biomedical sciences

2003 ◽  
Vol 17 (2-3) ◽  
pp. 255-273 ◽  
Author(s):  
Patrick Englebienne ◽  
Anne Van Hoonacker ◽  
Michel Verhas
Keyword(s):  
Surface Plasmon Resonance ◽  
Real Time ◽  
Metal Surface ◽  
Surface Plasmon ◽  
High Throughput ◽  
Plasmon Resonance ◽  
Colloidal Gold ◽  
Biomedical Applications ◽  
Clinical Chemistry ◽  
Label Free

Surface plasmon resonance (SPR) is a phenomenon occuring at metal surfaces (typically gold and silver) when an incident light beam strikes the surface at a particular angle. Depending on the thickness of a molecular layer at the metal surface, the SPR phenomenon results in a graded reduction in intensity of the reflected light. Biomedical applications take advantage of the exquisite sensitivity of SPR to the refractive index of the medium next to the metal surface, which makes it possible to measure accurately the adsorption of molecules on the metal surface and their eventual interactions with specific ligands. The last ten years have seen a tremendous development of SPR use in biomedical applications. The technique is applied not only to the measurement in real-time of the kinetics of ligand–receptor interactions and to the screening of lead compounds in the pharmaceutical industry, but also to the measurement of DNA hybridization, enzyme–substrate interactions, in polyclonal antibody characterization, epitope mapping, protein conformation studies and label-free immunoassays. Conventional SPR is applied in specialized biosensing instruments. These instruments use expensive sensor chips of limited reuse capacity and require complex chemistry for ligand or protein immobilization. Our laboratory has successfully applied SPR with colloidal gold particles in buffered solution. This application offers many advantages over conventional SPR. The support is cheap, easily synthesized, and can be coated with various proteins or protein–ligand complexes by charge adsorption. With colloidal gold, the SPR phenomenon can be monitored in any UV-vis spectrophotometer. For high‒throughput applications, we have adapted the technology in an automated clinical chemistry analyzer. This simple technology finds application in label-free quantitative immunoassay techniques for proteins and small analytes, in conformational studies with proteins as well as in the real-time association-dissociation measurements of receptor–ligand interactions, for high-throughput screening and lead optimization.

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