Gene Silencing with siRNA Encapsulated Nanoparticles to Overcome Tumor Multidrug Resistance

2011 ◽  
pp. 298-314
Keyword(s):  
Multidrug Resistance ◽  
Gene Silencing ◽  
Encapsulated Nanoparticles

scholarly journals Functionalized Graphene Oxide Mediated Adriamycin Delivery and miR-21 Gene Silencing to Overcome Tumor Multidrug Resistance In Vitro

PLoS ONE ◽  
2013 ◽  
Vol 8 (3) ◽  
pp. e60034 ◽  
Author(s):  
Feng Zhi ◽  
Haifeng Dong ◽  
Xuefeng Jia ◽  
Wenjie Guo ◽  
Huiting Lu ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Multidrug Resistance ◽  
Gene Silencing ◽  
Functionalized Graphene ◽  
Functionalized Graphene Oxide

Reversing Multidrug Resistance by Multiplexed Gene Silencing for Enhanced Breast Cancer Chemotherapy

2018 ◽  
Vol 10 (18) ◽  
pp. 15461-15466 ◽  
Author(s):  
Yanli Li ◽  
Xiaonan Gao ◽  
Zhengze Yu ◽  
Bo Liu ◽  
Wei Pan ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Multidrug Resistance ◽  
Gene Silencing ◽  
Cancer Chemotherapy ◽  
Breast Cancer Chemotherapy

scholarly journals Effect of Adenovirus-Mediated RNA Interference on Endogenous MicroRNAs in a Mouse Model of Multidrug Resistance Protein 2 Gene Silencing

2006 ◽  
Vol 80 (24) ◽  
pp. 12236-12247 ◽  
Author(s):  
Iñigo Narvaiza ◽  
Oscar Aparicio ◽  
María Vera ◽  
Nerea Razquin ◽  
Sergia Bortolanza ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Rna Interference ◽  
Gene Silencing ◽  
Viral Vectors ◽  
Minimal Amount ◽  
Multidrug Resistance Protein ◽  
Shrna Expression ◽  
Multidrug Resistance Protein 2 ◽  
Resistance Protein

ABSTRACT RNA interference with viral vectors that express short hairpin RNAs (shRNAs) has emerged as a powerful tool for functional genomics and therapeutic purposes. However, little is known about shRNA in vivo processing, accumulation, functional kinetics, and side effects related to shRNA saturation of the cellular gene silencing machinery. Therefore, we constructed first-generation recombinant adenoviruses encoding different shRNAs against murine ATP-binding cassette multidrug resistance protein 2 (Abcc2), which is involved in liver transport of bilirubin to bile, and analyzed Abcc2 silencing kinetics. C57/BL6 mice injected with these viruses showed significant impairment of Abcc2 function for up to 3 weeks, as reflected by increased serum bilirubin levels. The lack of Abcc2 function correlated with a specific reduction of Abcc2 mRNA and with high levels of processed shRNAs targeting Abcc2. Inhibition was lost at longer times postinfection, correlating with a decrease in the accumulation of processed shRNAs. This finding suggests that a minimal amount of processed shRNAs is required for efficient silencing in vivo. This system was also used to evaluate the effect of shRNA expression on the saturation of silencing factors. Saturation of the cellular silencing processing machinery alters the accumulation and functionality of endogenous microRNAs (miRNAs) and pre-miRNAs. However, expression of functional exogenous shRNAs did not change the levels of endogenous miRNAs or their precursors. In summary, this work shows that adenoviral vectors can deliver sufficient shRNAs to mediate inhibition of gene expression without saturating the silencing machinery.

Study of the mechanism responsible for multidrug resistance in breast cancer MCF-7 cell mediated by 14-3-3σ

2010 ◽  
Vol 34 (8) ◽  
pp. S47-S47
Author(s):  
Guopei Zheng ◽  
Sisi Yi ◽  
Yafei Li ◽  
Fangren Kong ◽  
Yanhui Yu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Multidrug Resistance ◽  
Mcf 7

Expression and localization of the multidrug resistance proteins MRP2 and MRP3 in human gallbladder epithelia

2001 ◽  
Vol 120 (5) ◽  
pp. A93-A93
Author(s):  
D ROST ◽  
J KONIG ◽  
G WEISS ◽  
E KLAR ◽  
W STREMMEL ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Multidrug Resistance Proteins ◽  
Human Gallbladder ◽  
Resistance Proteins

968: Gelsolin Gene Silencing Involving Unusual Chromatin Structures and a Novel Stem Loop Structure of the Promoter Region in Human Bladder Cancer

2004 ◽  
Vol 171 (4S) ◽  
pp. 256-257
Author(s):  
Kazunori Haga ◽  
Ataru Sazawa ◽  
Toru Harabayashi ◽  
Nobuo Shinohara ◽  
Minoru Nomoto ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Gene Silencing ◽  
Promoter Region ◽  
Loop Structure ◽  
Human Bladder Cancer ◽  
Human Bladder ◽  
Stem Loop ◽  
Stem Loop Structure
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