Genome Mapping and QTL Analysis in Cucurbits

2011 ◽  
pp. 238-285
Author(s):  
Hugo Cuevas ◽  
Jack Staub ◽  
Juan Zalapa
Keyword(s):  
Author(s):  
B.A. Hamkalo ◽  
S. Narayanswami ◽  
A.P. Kausch

The availability of nonradioactive methods to label nucleic acids an the resultant rapid and greater sensitivity of detection has catapulted the technique of in situ hybridization to become the method of choice to locate of specific DNA and RNA sequences on chromosomes and in whole cells in cytological preparations in many areas of biology. It is being applied to problems of fundamental interest to basic cell and molecular biologists such as the organization of the interphase nucleus in the context of putative functional domains; it is making major contributions to genome mapping efforts; and it is being applied to the analysis of clinical specimens. Although fluorescence detection of nucleic acid hybrids is routinely used, certain questions require greater resolution. For example, very closely linked sequences may not be separable using fluorescence; the precise location of sequences with respect to chromosome structures may be below the resolution of light microscopy(LM); and the relative positions of sequences on very small chromosomes may not be feasible.


2009 ◽  
Vol 35 (1) ◽  
pp. 57-65 ◽  
Author(s):  
Rong ZHOU ◽  
Xian-Zhi WANG ◽  
Hai-Feng CHEN ◽  
Xiao-Juan ZHANG ◽  
Zhi-Hui SHAN ◽  
...  
Keyword(s):  

2010 ◽  
Vol 36 (2) ◽  
pp. 242-248 ◽  
Author(s):  
Guang-Jun LI ◽  
He-Nan LI ◽  
Li-Guo CHENG ◽  
Yuan-Ming ZHANG

2018 ◽  
Vol 44 (4) ◽  
pp. 522
Author(s):  
Hai-Ping GUO ◽  
Gao-Yang SUN ◽  
Xiao-Xiang ZHANG ◽  
Peng-Shuai YAN ◽  
Kun LIU ◽  
...  

2011 ◽  
Vol 37 (9) ◽  
pp. 1577-1584 ◽  
Author(s):  
Hua-Li LI ◽  
Mei-Xia CHEN ◽  
Dong-Xin ZHOU ◽  
Shun-Hui CHEN ◽  
Ai-Fen TAO ◽  
...  

2011 ◽  
Vol 37 (9) ◽  
pp. 1525-1532 ◽  
Author(s):  
Yue FENG ◽  
Rong-Rong ZHAI ◽  
Li-Yong CAO ◽  
Ze-Chuan LIN ◽  
Xing-Hua WEI ◽  
...  

2016 ◽  
Vol 42 (2) ◽  
pp. 303
Author(s):  
Ya-Kun LEI ◽  
Bing-Qiang LIU ◽  
Rui DI ◽  
Long YAN ◽  
Chun-Yan YANG ◽  
...  
Keyword(s):  

2011 ◽  
Vol 37 (8) ◽  
pp. 1342-1350 ◽  
Author(s):  
Yan-Yu YANG ◽  
Sheng-Qiang YANG ◽  
Zhe-Hong CHEN ◽  
Chun-Yun GUAN ◽  
She-Yuan CHEN ◽  
...  

2010 ◽  
Vol 36 (11) ◽  
pp. 1832-1842 ◽  
Author(s):  
Bo PENG ◽  
Yang WANG ◽  
Yong-Xiang LI ◽  
Cheng LIU ◽  
Zhi-Zhai LIU ◽  
...  

2020 ◽  
Vol 110 (9) ◽  
pp. 1511-1521
Author(s):  
Juliet Wilkes ◽  
Christopher Saski ◽  
Mariola Klepadlo ◽  
Benjamin Fallen ◽  
Paula Agudelo

Reniform nematode (Rotylenchulus reniformis) is a yield-limiting pathogen of soybean (Glycine max) in the southeastern region of the United States. A population of 250 recombinant inbred lines (RIL) (F2:8) developed from a cross between reniform nematode resistant soybean cultivar Forrest and susceptible cultivar Williams 82 was utilized to identify regions associated with host suitability. A genetic linkage map was constructed using single-nucleotide polymorphism markers generated by genotyping-by-sequencing. The phenotype was measured in the RIL population and resistance was characterized using normalized and transformed nematode reproduction indices in an optimal univariate cluster analysis. Quantitative trait loci (QTL) analysis using normalized phenotype scores identified two QTLs on each arm of chromosome 18 (rrn-1 and rrn-2). The same QTL analysis performed with log10(x) transformed phenotype data also identified two QTLs: one on chromosome 18 overlapping the same region in the other analysis (rrn-1), and one on chromosome 11 (rrn-3). While rrn-1 and rrn-3 have been reported associated with reduced reproduction of reniform nematode, this is the first report of the rrn-2 region associated with host suitability to reniform nematode. The resistant parent allele at rrn-2 showed an inverse relationship with the resistance phenotype, correlating with an increase in nematode reproduction or host suitability. Several candidate genes within these regions corresponded with host plant defense systems. Interestingly, a characteristic pathogen resistance gene with a leucine-rich repeat was discovered within rrn-2. These genetic markers can be used by soybean breeders in marker-assisted selection to develop lines with resistance to reniform nematode.


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