Methods of Stem Cell Lineage Analysis In Situ and in Cell Culture

2016 ◽  
pp. 600-613
Keyword(s):  
Cell Culture ◽  
Stem Cell ◽  
Cell Lineage ◽  
Stem Cell Lineage ◽  
Lineage Analysis

scholarly journals StemMapper: a curated gene expression database for stem cell lineage analysis

2017 ◽  
Vol 46 (D1) ◽  
pp. D788-D793 ◽  
Author(s):  
José P Pinto ◽  
Rui S R Machado ◽  
Ramiro Magno ◽  
Daniel V Oliveira ◽  
Susana Machado ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cell ◽  
Cell Lineage ◽  
Gene Expression Database ◽  
Stem Cell Lineage ◽  
Lineage Analysis

Lox2, a putative leech segment identity gene, is expressed in the same segmental domain in different stem cell lineages

Development ◽  
1992 ◽  
Vol 116 (3) ◽  
pp. 697-710 ◽  
Author(s):  
D. Nardelli-Haefliger ◽  
M. Shankland
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Embryonic Stem ◽  
Cell Lineage ◽  
Gene Products ◽  
Cell Lineages ◽  
Stem Cell Lineage ◽  
Hybridization Reaction ◽  
Cell Segment

The segmented tissues of the adult leech arise from a set of five, bilaterally paired embryonic stem cells via a stereotyped sequence of cell lineage. Individual segments exhibit unique patterns of cell differentiation, and previous studies have suggested that each stem cell lineage establishes at least some aspects of its own segmental specificity autonomously. In this paper, we describe a putative leech segment identity gene, Lox2, and examine its expression in the various stem cell lineages. Both sequence analysis and the segmental pattern of Lox2 expression suggest a specific homology to the fruitfly segment identity genes Ubx and abdA. In situ hybridization reveals a cellular accumulation of Lox2 RNA over a contiguous domain of 16 midbody segments (M6-M21), including postmitotic neurons, muscles and the differentiating genitalia. Lox2 transcripts were not detected at the stage when segment identities are first established, suggesting that Lox2 gene products may not be part of the initial specification process. Individual stem cell lineages were labeled by intracellular injection of fluorescent tracers, and single cell colocalization of lineage tracer and hybridization reaction product revealed expression of Lox2 RNA in the progeny of four different stem cells. The segmental domain of Lox2 RNA was very similar in the various stem cell lineages, despite the fact that some stem cells generate one founder cell/segment, whereas other stem cells generate two founder cells/segment.

scholarly journals Clonal reversal of ageing-associated stem cell lineage bias via a pluripotent intermediate

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Martin Wahlestedt ◽  
Eva Erlandsson ◽  
Trine Kristiansen ◽  
Rong Lu ◽  
Cord Brakebusch ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Lineage ◽  
Stem Cell Lineage
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