Dynamics in Diamagnetic Materials from Solid-State NMR: Methods, Measurements, and Analysis

2016 ◽  
pp. 156-181
Keyword(s):  
Solid State ◽  
Solid State Nmr ◽  
Nmr Methods

Solid state NMR of membrane proteins: methods and applications

2021 ◽  
Author(s):  
Vivien Yeh ◽  
Boyan B. Bonev
Keyword(s):  
Solid State ◽  
High Resolution ◽  
Membrane Proteins ◽  
Solid State Nmr ◽  
Lipid Membrane ◽  
Cell Function ◽  
Membrane Lipid ◽  
Pharmacological Intervention ◽  
Nmr Methods ◽  
Informative Approach

Membranes of cells are active barriers, in which membrane proteins perform essential remodelling, transport and recognition functions that are vital to cells. Membrane proteins are key regulatory components of cells and represent essential targets for the modulation of cell function and pharmacological intervention. However, novel folds, low molarity and the need for lipid membrane support present serious challenges to the characterisation of their structure and interactions. We describe the use of solid state NMR as a versatile and informative approach for membrane and membrane protein studies, which uniquely provides information on structure, interactions and dynamics of membrane proteins. High resolution approaches are discussed in conjunction with applications of NMR methods to studies of membrane lipid and protein structure and interactions. Signal enhancement in high resolution NMR spectra through DNP is discussed as a tool for whole cell and interaction studies.

Protein structure by solid-state NMR spectroscopy

1987 ◽  
Vol 19 (1-2) ◽  
pp. 7-49 ◽  
Author(s):  
S. J. Opella ◽  
P. L. Stewart ◽  
K. G. Valentine
Keyword(s):  
Solid State ◽  
Nmr Spectroscopy ◽  
Solid State Nmr ◽  
Structural Information ◽  
Biological Systems ◽  
Genetic Regulation ◽  
Protein Structures ◽  
Crystalline Solid ◽  
Solid State Nmr Spectroscopy ◽  
Nmr Methods

The three-dimensional structures of proteins are among the most valuable contributions of biophysics to the understanding of biological systems (Dickerson & Geis, 1969; Creighton, 1983). Protein structures are utilized in the description and interpretation of a wide variety of biological phenomena, including genetic regulation, enzyme mechanisms, antibody recognition, cellular energetics, and macroscopic mechanical and structural properties of molecular assemblies. Virtually all of the information currently available about the structures of proteins at atomic resolution has been obtained from diffraction studies of single crystals of proteins (Wyckoff et al, 1985). However, recently developed NMR methods are capable of determining the structures of proteins and are now being applied to a variety of systems, including proteins in solution and other non-crystalline environments that are not amenable for X-ray diffraction studies. Solid-state NMR methods are useful for proteins that undergo limited overall reorientation by virtue of their being in the crystalline solid state or integral parts of supramolecular structures that do not reorient rapidly in solution. For reviews of applications of solid-state NMR spectroscopy to biological systems see Torchia and VanderHart (1979), Griffin (1981), Oldfield et al. (1982), Opella (1982), Torchia (1982), Gauesh (1984), Torchia (1984) and Opella (1986). This review describes how solid-state NMR can be used to obtain structural information about proteins. Methods applicable to samples with macroscopic orientation are emphasized.

scholarly journals Solid-State NMR Techniques for the Structural Characterization of Cyclic Aggregates Based on Borane–Phosphane Frustrated Lewis Pairs

Molecules ◽  
2020 ◽  
Vol 25 (6) ◽  
pp. 1400 ◽  
Author(s):  
Robert Knitsch ◽  
Melanie Brinkkötter ◽  
Thomas Wiegand ◽  
Gerald Kehr ◽  
Gerhard Erker ◽  
...  
Keyword(s):  
Solid State ◽  
Structural Characterization ◽  
Solid State Nmr ◽  
Magic Angle ◽  
Frustrated Lewis Pairs ◽  
Wide Range ◽  
Nmr Techniques ◽  
Nmr Methods ◽  
Lewis Pairs

Modern solid-state NMR techniques offer a wide range of opportunities for the structural characterization of frustrated Lewis pairs (FLPs), their aggregates, and the products of cooperative addition reactions at their two Lewis centers. This information is extremely valuable for materials that elude structural characterization by X-ray diffraction because of their nanocrystalline or amorphous character, (pseudo-)polymorphism, or other types of disordering phenomena inherent in the solid state. Aside from simple chemical shift measurements using single-pulse or cross-polarization/magic-angle spinning NMR detection techniques, the availability of advanced multidimensional and double-resonance NMR methods greatly deepened the informational content of these experiments. In particular, methods quantifying the magnetic dipole–dipole interaction strengths and indirect spin–spin interactions prove useful for the measurement of intermolecular association, connectivity, assessment of FLP–ligand distributions, and the stereochemistry of adducts. The present review illustrates several important solid-state NMR methods with some insightful applications to open questions in FLP chemistry, with a particular focus on supramolecular associates.

Network Former Mixing (NFM) Effects in Ion-Conducting Glasses - Structure/Property Correlations Studied by Modern Solid-State NMR Techniques

2016 ◽  
Vol 6 ◽  
pp. 144-193 ◽  
Author(s):  
Hellmut Eckert
Keyword(s):  
Solid State ◽  
Solid State Nmr ◽  
Glass Structure ◽  
Energy Storage And Conversion ◽  
Structure Property ◽  
Local Environments ◽  
Ion Conducting ◽  
Multiple Network ◽  
Nmr Methods ◽  
Property Correlations

Glassy solid electrolytes are important integral components for all-solid-state devices for energy storage and conversion. The use of multiple network formers is an important part of their design strategy for specific applications. In many glass systems the interaction between the different network formers results in strongly non-linear variations in physical properties (network former mixing (NFM) effects), requiring a detailed understanding on a structural basis.The issues to be addressed involve both the structural organization and connectivities within the framework, the local environments and spatial distributions of the mobile ions, and the dynamical aspects of ion transport, to be discussed in relation to possible phase separation or nano-segregation effects. Besides Raman and X-ray photoelectron spectroscopies, solid state nuclear magnetic resonance (NMR) methods are particularly useful for providing detailed answers to such issues. The present review introduces the basic principles of modern solid state NMR methods and their applications to glass structure, with a particular focus on the characterization of network-former mixing effects in the most common lithium and sodium conducting oxide and chalcogenide glass systems. Based on the current state of the literature reviewed in the present work, some emerging general principles governing structure/property correlations are identified, to be tested by further experimenteation in the future.

Understanding the Molecular Dynamics Associated with Polymorphic Transitions ofdl-Norvaline with Solid-State NMR Methods

2011 ◽  
Vol 115 (12) ◽  
pp. 2814-2823 ◽  
Author(s):  
Pingping Ren ◽  
Detlef Reichert ◽  
Qinghua He ◽  
Limin Zhang ◽  
Huiru Tang
Keyword(s):  
Molecular Dynamics ◽  
Solid State ◽  
Solid State Nmr ◽  
Polymorphic Transitions ◽  
Nmr Methods

scholarly journals Complex F,Cl Apatite Solid Solution Investigated Using Multinuclear Solid-State NMR Methods

2017 ◽  
Vol 122 (1) ◽  
pp. 530-539 ◽  
Author(s):  
John S. Vaughn ◽  
Donald H. Lindsley ◽  
Hanna Nekvasil ◽  
John M. Hughes ◽  
Brian L. Phillips
Keyword(s):  
Solid Solution ◽  
Solid State ◽  
Solid State Nmr ◽  
Nmr Methods
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