Application of Infrared and Raman Spectroscopy for Detection in Liquid Chromatographic Separations

2011 ◽  
pp. 99-144
Author(s):  
Peter J. Mahon
Keyword(s):  
Raman Spectroscopy ◽  
Chromatographic Separations ◽  
Infrared And Raman Spectroscopy ◽  
Liquid Chromatographic

Identification of filler substances in emeralds by infrared and Raman spectroscopy

2000 ◽  
Vol 26 (1) ◽  
pp. 501-520 ◽  
Author(s):  
L. Kiefert ◽  
H.A. Hänni ◽  
J-P. Chalain ◽  
W. Weber
Keyword(s):  
Raman Spectroscopy ◽  
Infrared And Raman Spectroscopy

Eco-Friendly Green Liquid Chromatographic Separations of a Novel Combination of Azelastine and Fluticasone in the Presence of their Pharmaceutical Dosage form Additives

2020 ◽  
Vol 16 (3) ◽  
pp. 277-286
Author(s):  
Amal A. El-Masry ◽  
Mohammed E. A. Hammouda ◽  
Dalia R. El-Wasseef ◽  
Saadia M. El-Ashry
Keyword(s):  
Lower Limit ◽  
Dosage Form ◽  
Limit Of Detection ◽  
Sodium Dodecyl ◽  
Uv Detection ◽  
Simultaneous Estimation ◽  
Limit Of Quantification ◽  
Chromatographic Separations ◽  
Pharmaceutical Dosage Form ◽  
Liquid Chromatographic

Background: The first highly sensitive, rapid and specific green microemulsion liquid chromatographic (MELC) method was established for the simultaneous estimation of fluticasone propionate (FLU) and azelastine HCl (AZL) in the presence of their pharmaceutical dosage form additives (phenylethyl alcohol (PEA) and benzalkonium chloride (BNZ)). Methods: The separation was performed on a C18 column using (o/w) microemulsion as a mobile phase which contains 0.2 M sodium dodecyl sulphate (SDS) as surfactant, 10% butanol as cosurfactant, 1% n-octanol as internal phase and 0.3% triethylamine (TEA) adjusted at pH 6 by 0.02 M phosphoric acid; with UV detection at 220 nm and programmed with flow rate of 1 mL/min. Results: The validation characteristics e.g. linearity, lower limit of quantification (LOQ), lower limit of detection (LOD), accuracy, precision, robustness and specificity were investigated. The proposed method showed linearity over the concentration range of (0.5-25 µg/mL) and (0.1-25 µg/mL) for FLU and AZL, respectively. Besides that, the method was adopted in a short chromatographic run with satisfactory resolution factors of (2.39, 3.78 and 6.74 between PEA/FLU, FLU/AZL and AZL/BNZ), respectively. The performed method was efficiently applied to pharmaceutical nasal spray with (mean recoveries ± SD) (99.80 ± 0.97) and (100.26 ± 0.96) for FLU and AZL, respectively. Conclusion: The suggested method was based on simultaneous determination of FLU and AZL in the presence of PEA and BNZ in pure form, laboratory synthetic mixture and its combined pharmaceutical dosage form using green MELC technique with UV detection. The proposed method appeared to be superior to the reported ones of being more sensitive and specific, as well as the separation was achieved with good performance in a relatively short analysis time (less than 7.5 min). Highly acceptable values of LOD and % RSD make this method superior to be used in quality control laboratories with of HPLC technique.

scholarly journals Multivariate approach for the authentication of vanilla using infrared and Raman spectroscopy

2021 ◽  
Vol 141 ◽  
pp. 110196
Author(s):  
Ana M. Jiménez-Carvelo ◽  
Margherita Tonolini ◽  
Orla McAleer ◽  
Luis Cuadros-Rodríguez ◽  
Daniel Granato ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Multivariate Approach ◽  
Infrared And Raman Spectroscopy

scholarly journals Authentication of the Botanical and Geographical Origin and Detection of Adulteration of Olive Oil Using Gas Chromatography, Infrared and Raman Spectroscopy Techniques: A Review

Foods ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1565
Author(s):  
Eleni Kakouri ◽  
Panagiota-Kyriaki Revelou ◽  
Charalabos Kanakis ◽  
Dimitra Daferera ◽  
Christos S. Pappas ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Raman Spectroscopy ◽  
Olive Oil ◽  
Nutritional Value ◽  
Review Paper ◽  
Geographical Origin ◽  
Analytical Techniques ◽  
Volatile Fraction ◽  
Olive Oils ◽  
Infrared And Raman Spectroscopy

Olive oil is among the most popular supplements of the Mediterranean diet due to its high nutritional value. However, at the same time, because of economical purposes, it is also one of the products most subjected to adulteration. As a result, authenticity is an important issue of concern among authorities. Many analytical techniques, able to detect adulteration of olive oil, to identify its geographical and botanical origin and consequently guarantee its quality and authenticity, have been developed. This review paper discusses the use of infrared and Raman spectroscopy as candidate tools to examine the authenticity of olive oils. It also considers the volatile fraction as a marker to distinguish between different varieties and adulterated olive oils, using SPME combined with gas chromatography technique.

scholarly journals Infrared and Raman Spectroscopy

2005 ◽  
Vol 10 (3) ◽  
pp. 031101 ◽  
Author(s):  
Michael D. Morris ◽  
Andrew Berger ◽  
Anita Mahadevan-Jansen
Keyword(s):  
Raman Spectroscopy ◽  
Infrared And Raman Spectroscopy

The Fluorescence of Diamond and Raman Spectroscopy at High Pressures Using a New Design of Diamond Anvil Cell

1973 ◽  
Vol 27 (5) ◽  
pp. 377-381 ◽  
Author(s):  
D. M. Adams ◽  
S. J. Payne ◽  
K. Martin
Keyword(s):  
Raman Spectroscopy ◽  
Diamond Anvil Cell ◽  
High Pressures ◽  
Fluorescence Emission ◽  
Type I ◽  
High Pressure Cell ◽  
Scattering Geometry ◽  
Infrared And Raman Spectroscopy ◽  
Bond Stretching ◽  
Diamond Anvil

A new design of diamond anvil high pressure cell suitable for use in infrared and Raman spectroscopy is described. Its performance is demonstrated with particular reference to the pressure dependence of the infrared spectrum of K2PtCl6 and the Raman spectrum of W(CO)6. In contrast to earlier reports, in which forward scattering geometry was used, this design of cell is shown to be very suitable for Raman use in the 180° excitation mode. However, severe limitations are imposed by the fluorescence emission of diamond and of sapphire. Conditions under which the cell can be used for Raman work are summarized. New fluorescence and Raman features are reported for diamond. In particular, a band at 1730 cm−1 is characteristic of type I stones and may be due to C to N bond stretching at defect centers.

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