scholarly journals Biomimicry at the Cell–Material Interface

Biomimetics ◽  
2016 ◽  
pp. 115-150
Keyword(s):  
Material Interface ◽  
Cell Material

scholarly journals Biomaterial substrate modifications that influence cell-material interactions to prime cellular responses to nonviral gene delivery

2019 ◽  
Vol 244 (2) ◽  
pp. 100-113 ◽  
Author(s):  
Amy Mantz ◽  
Angela K Pannier
Keyword(s):  
Gene Delivery ◽  
Intracellular Trafficking ◽  
Cellular Response ◽  
Adhesion Formation ◽  
Nonviral Gene Delivery ◽  
Material Interface ◽  
Cytoskeletal Remodeling ◽  
Cell Material ◽  
Focal Adhesion Formation ◽  
A Cell

Gene delivery is the transfer of exogenous genetic material into somatic cells to modify their gene expression, with applications including tissue engineering, regenerative medicine, sensors and diagnostics, and gene therapy. Viral vectors are considered the most effective system to deliver nucleic acids, yet safety concerns and many other disadvantages have resulted in investigations into an alternative option, i.e. nonviral gene delivery. Chemical nonviral gene delivery is typically accomplished by electrostatically complexing cationic lipids or polymers with negatively charged nucleic acids. Unfortunately, nonviral gene delivery suffers from low efficiency due to barriers that impede transfection success, including intracellular processes such as internalization, endosomal escape, cytosolic trafficking, and nuclear entry. Efforts to improve nonviral gene delivery have focused on modifying nonviral vectors, yet a novel solution that may prove more effective than vector modifications is stimulating or “priming” cells before transfection to modulate and mitigate the cellular response to nonviral gene delivery. In applications where a cell-material interface exists, cell priming can come from cues from the substrate, through chemical modifications such as the addition of natural coatings, ligands, or functional side groups, and/or physical modifications such as topography or stiffness, to mimic extracellular matrix cues and modulate cellular behaviors that influence transfection efficiency. This review summarizes how biomaterial substrate modifications can prime the cellular response to nonviral gene delivery (e.g. integrin binding and focal adhesion formation, cytoskeletal remodeling, endocytic mechanisms, intracellular trafficking) to aid in improving gene delivery for future therapeutic applications. Impact statement This review summarizes how biomaterial substrate modifications (e.g. chemical modifications like natural coatings, ligands, or functional side groups, and/or physical modifications such as topography or stiffness) can prime the cellular response to nonviral gene delivery (e.g. affecting integrin binding and focal adhesion formation, cytoskeletal remodeling, endocytic mechanisms, and intracellular trafficking), to aid in improving gene delivery for applications where a cell-material interface might exist (e.g. tissue engineering scaffolds, medical implants and devices, sensors and diagnostics, wound dressings).

scholarly journals Role of superhydrophobicity in the biological activity of fibronectin at the cell–material interface

Soft Matter ◽  
2011 ◽  
Vol 7 (22) ◽  
pp. 10803 ◽  
Author(s):  
José Ballester-Beltrán ◽  
Patricia Rico ◽  
David Moratal ◽  
Wenlong Song ◽  
João F. Mano ◽  
...  
Keyword(s):  
Biological Activity ◽  
Material Interface ◽  
Cell Material

scholarly journals Revealing the Cell–Material Interface with Nanometer Resolution by Focused Ion Beam/Scanning Electron Microscopy

ACS Nano ◽  
2017 ◽  
Vol 11 (8) ◽  
pp. 8320-8328 ◽  
Author(s):  
Francesca Santoro ◽  
Wenting Zhao ◽  
Lydia-Marie Joubert ◽  
Liting Duan ◽  
Jan Schnitker ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Focused Ion Beam ◽  
Ion Beam ◽  
Beam Scanning ◽  
Material Interface ◽  
Nanometer Resolution ◽  
Cell Material ◽  
Scanning Electron

scholarly journals Lateral Chain Length in Polyalkyl Acrylates Determines the Mobility of Fibronectin at the Cell/Material Interface

Langmuir ◽  
2016 ◽  
Vol 32 (3) ◽  
pp. 800-809 ◽  
Author(s):  
Fatma Bathawab ◽  
Mark Bennett ◽  
Marco Cantini ◽  
Julien Reboud ◽  
Matthew J. Dalby ◽  
...  
Keyword(s):  
Chain Length ◽  
Material Interface ◽  
Cell Material

scholarly journals Revealing the cell-material interface with nanometer resolution by FIB-SEM

2017 ◽  
Author(s):  
Francesca Santoro ◽  
Wenting Zhao ◽  
Lydia-Marie Joubert ◽  
Liting Duan ◽  
Jan Schnitker ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Focused Ion Beam ◽  
Ion Beam ◽  
Titanium Implants ◽  
Chemical Compositions ◽  
Material Interface ◽  
Microscopy Imaging ◽  
Substrate Removal ◽  
Cell Material

The interface between biological cells and non-biological surfaces profoundly influences cellular activities, chronic tissue responses, and ultimately the success of medical implants. Materials in contact with cells can be plastics, metal, ceramics or other synthetic materials, and their surfaces vary widely in chemical compositions, stiffness, topography and levels of roughness. To understand the molecular mechanism of how cells and tissues respond to different materials, it is of critical importance to directly visualize the cell-material interface at the relevant length scale of nanometers. Conventional ultrastructural analysis by transmission electron microscopy (TEM) often requires substrate removal before microtome sectioning, which is not only challenging for most substrates but also can cause structural distortions of the interface. Here, we present a new method for in situ examination of the cell-to-material interface at any desired cellular location, based on focused-ion beam milling and scanning electron microscopy imaging (FIB-SEM). This method involves a thin-layer plastification procedure that preserves adherent cells as well as enhances the contrast of biological specimen. We demonstrate that this unique procedure allows the visualization of cell-to-material interface and intracellular structures with 10nm resolution, compatible with a variety of materials and surface topographies, and capable of volume and multi-directional imaging. We expect that this method will be very useful for studies of cell-to-material interactions and also suitable for in vivo studies such as examining osteoblast adhesion and new bone formation in response to titanium implants.

Vitronectin alters fibronectin organization at the cell–material interface

2013 ◽  
Vol 111 ◽  
pp. 618-625 ◽  
Author(s):  
Cristina González-García ◽  
Marco Cantini ◽  
David Moratal ◽  
George Altankov ◽  
Manuel Salmerón-Sánchez
Keyword(s):  
Material Interface ◽  
Cell Material
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