Combinatorial Biosynthesis of Anticancer Natural Products

2011 ◽  
pp. 687-714
Keyword(s):  
Natural Products ◽  
Combinatorial Biosynthesis

Expanding the Natural Products Heterologous Expression Repertoire in the Model Cyanobacterium Anabaena sp. Strain PCC 7120: Production of Pendolmycin and Teleocidin B-4

2019 ◽  
Author(s):  
Patrick Videau ◽  
Kaitlyn Wells ◽  
Arun Singh ◽  
Jessie Eiting ◽  
Philip Proteau ◽  
...  
Keyword(s):  
Natural Products ◽  
Genome Mining ◽  
Gene Clusters ◽  
Combinatorial Biosynthesis ◽  
Test Case ◽  
Biosynthetic Gene ◽  
Biosynthetic Gene Clusters ◽  
Cyanobacterium Anabaena ◽  
Anabaena Sp ◽  
Pcc 7120

Cyanobacteria are prolific producers of natural products and genome mining has shown that many orphan biosynthetic gene clusters can be found in sequenced cyanobacterial genomes. New tools and methodologies are required to investigate these biosynthetic gene clusters and here we present the use of <i>Anabaena </i>sp. strain PCC 7120 as a host for combinatorial biosynthesis of natural products using the indolactam natural products (lyngbyatoxin A, pendolmycin, and teleocidin B-4) as a test case. We were able to successfully produce all three compounds using codon optimized genes from Actinobacteria. We also introduce a new plasmid backbone based on the native <i>Anabaena</i>7120 plasmid pCC7120ζ and show that production of teleocidin B-4 can be accomplished using a two-plasmid system, which can be introduced by co-conjugation.

scholarly journals Probing the promiscuity of ent-kaurene oxidases via combinatorial biosynthesis

2016 ◽  
Vol 113 (9) ◽  
pp. 2526-2531 ◽  
Author(s):  
Sibongile Mafu ◽  
Meirong Jia ◽  
Jiachen Zi ◽  
Dana Morrone ◽  
Yisheng Wu ◽  
...  
Keyword(s):  
Natural Products ◽  
Metabolic Engineering ◽  
Substrate Specificity ◽  
Plant Hormone ◽  
Structural Similarity ◽  
Combinatorial Biosynthesis ◽  
Underlying Structure ◽  
Engineering System ◽  
Insight Into

The substrate specificity of enzymes from natural products’ metabolism is a topic of considerable interest, with potential biotechnological use implicit in the discovery of promiscuous enzymes. However, such studies are often limited by the availability of substrates and authentic standards for identification of the resulting products. Here, a modular metabolic engineering system is used in a combinatorial biosynthetic approach toward alleviating this restriction. In particular, for studies of the multiply reactive cytochrome P450, ent-kaurene oxidase (KO), which is involved in production of the diterpenoid plant hormone gibberellin. Many, but not all, plants make a variety of related diterpenes, whose structural similarity to ent-kaurene makes them potential substrates for KO. Use of combinatorial biosynthesis enabled analysis of more than 20 such potential substrates, as well as structural characterization of 12 resulting unknown products, providing some insight into the underlying structure–function relationships. These results highlight the utility of this approach for investigating the substrate specificity of enzymes from complex natural products’ biosynthesis.

ChemInform Abstract: Glycosylated Natural Products: Perspectives for Combinatorial Biosynthesis

ChemInform ◽  
2010 ◽  
Vol 30 (49) ◽  
pp. no-no
Author(s):  
A. Bechthold ◽  
S. Domann ◽  
B. Faust ◽  
D. Hoffmeister ◽  
S. Stockert ◽  
...  
Keyword(s):  
Natural Products ◽  
Combinatorial Biosynthesis

scholarly journals Synthetic biology approaches and combinatorial biosynthesis towards heterologous lipopeptide production

2018 ◽  
Vol 9 (38) ◽  
pp. 7510-7519 ◽  
Author(s):  
Fu Yan ◽  
Christian Burgard ◽  
Alexander Popoff ◽  
Nestor Zaburannyi ◽  
Gregor Zipf ◽  
...  
Keyword(s):  
Natural Products ◽  
Synthetic Biology ◽  
Secondary Metabolite ◽  
Combinatorial Biosynthesis ◽  
Secondary Metabolite Production ◽  
Metabolite Production

Synthetic biology techniques coupled with heterologous secondary metabolite production offer opportunities for the discovery and optimisation of natural products.

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