Using CAGE Data for Quantitative Expression

2009 ◽  
pp. 101-121
Author(s):  
Michiel De Hoon ◽  
Nicolas Bertin ◽  
Alistair Chalk
Keyword(s):  
Quantitative Expression

1294: Quantitative Expression of Muscarinic Receptors in Urothelium and Detrusor of Healthy and Neurogenic Human Bladder

2006 ◽  
Vol 175 (4S) ◽  
pp. 417-417
Author(s):  
Shachi Tyagi ◽  
Naoki Yoshimura ◽  
Michael B. Chancellor ◽  
Fernando De Miguel
Keyword(s):  
Muscarinic Receptors ◽  
Human Bladder ◽  
Quantitative Expression

Assays for Phospholipid-Dependent Formation of Thrombin and Xa: A Potential Method for Quantifying Lupus Anticoagulant Activity

1991 ◽  
Vol 66 (04) ◽  
pp. 453-458 ◽  
Author(s):  
John T Brandt
Keyword(s):  
Specific Activity ◽  
Anticoagulant Activity ◽  
Factor Xa ◽  
Clinical Importance ◽  
Potential Method ◽  
Quantitative Expression ◽  
Increased Risk ◽  
Apparent Association

SummaryLupus anticoagulants (LAs) are antibodies which interfere with phospholipid-dependent procoagulant reactions. Their clinical importance is due to their apparent association with an increased risk of thrombo-embolic disease. To date there have been few assays for quantifying the specific activity of these antibodies in vitro and this has hampered attempts to purify and characterize these antibodies. Methods for determining phospholipid-dependent generation of thrombin and factor Xa are described. Isolated IgG fractions from 7 of 9 patients with LAs were found to reproducibly inhibit enzyme generation in these assay systems, permitting quantitative expression of inhibitor activity. Different patterns of inhibitory activity, based on the relative inhibition of thrombin and factor Xa generation, were found, further substantiating the known heterogeneity of these antibodies. These systems may prove helpful in further purification and characterization of LAs.

scholarly journals POS0500 NUMBER AND CO-EXPRESSION OF TNF RECEPTORS TYPE 1 AND 2 ON IMMUNOCOMPETENT CELLS ARE ASSOCIATED WITH STABILITY OF REMISSION IN RHEUMATOID ARTHRITIS PATIENTS

2021 ◽  
Vol 80 (Suppl 1) ◽  
pp. 482.2-483
Author(s):  
A. Alshevskaya ◽  
J. Lopatnikova ◽  
J. Zhukova ◽  
O. Chumasova ◽  
N. Shkaruba ◽  
...  
Keyword(s):  
Disease Activity ◽  
T Regulatory Cells ◽  
Lower Percentage ◽  
Tnf Receptors ◽  
Double Positive ◽  
Low Disease Activity ◽  
Quantitative Expression ◽  
Healthy Donors

Background:The balance of TNFα receptors expression on cells which are actively involved in immunopathological processes affects both the density of distribution of receptors on cells and co-expression in subsets. Previously it was shown that basic effective RA therapy with methotrexate and glucocorticoids leads to equalization of the expression profile either in the percentage of cells or in the number of receptors, approaching those of healthy donors, but not simultaneously. However, questions about the relationship between the effectiveness of biological therapy and receptors co-expression remain unknown.Objectives:To assess the differences in co-expression and quantitative expression of TNF receptors type 1 and 2 in subsets of cells associated with the severity of the disease, depending on the response to rituximab therapy.Methods:Subanalysis of patients with high disease activity level successfully treated with rituximab (alone or in combination treatment scheme) during hospitalization was performed (n = 14). The first group included 6 patients who retained low disease activity during 1 month follow-up (RA, stabilization). The second group consisted of 8 patients who had exacerbation during follow-up period. As a control group, we used data from 43 comparable healthy donors. Subsets of T regulatory cells and monocytes were studied. A comparison was made among the indicators of receptors number and proportion of cells expressing the corresponding receptor.Results:For T regulatory cells, the key differences for patients who did not retain low disease activity were significantly higher number of TNF type 1 and type 2 receptors on double-positive cells with a lower percentage of these cells compared to stable patients. At the same time, higher differences between proportions of double-positive cells in comparison with control values of healthy donors were associated with higher probability of maintaining in remission.For monocytes, the key differences in stable patients were the very high quantitative expression of type 1 receptors on double-positive cells, with a lower percentage of these cells compared to patients with exacerbation. At the same time, lower differences between proportions of double-positive cells in comparison with control values of healthy donors were associated with higher probability of maintaining in remission.Conclusion:Obtained data confirm the previously proposed hypothesis about the essential role of balance in quantitative expression of TNF receptors type 1 and 2 on double-positive cells to determine the intensity and type of cell response to the mediator and its association with the level of disease activity and response to therapy.Acknowledgements:This study is supported by grant of the President of the Russian Federation for state support of young Russian PhD scientists №МК-2433.2020.4Disclosure of Interests:None declared

Growth, nisA Gene Expression, and In Situ Activity of Novel Lactococcus lactis subsp. cremoris Costarter Culture in Commercial Hard Cheese Production

2017 ◽  
Vol 80 (12) ◽  
pp. 2137-2146 ◽  
Author(s):  
Dimitrios Noutsopoulos ◽  
Athanasia Kakouri ◽  
Eleftheria Kartezini ◽  
Dimitrios Pappas ◽  
Efstathios Hatziloukas ◽  
...  
Keyword(s):  
Gene Expression ◽  
Lactococcus Lactis ◽  
Starter Culture ◽  
Fold Increase ◽  
Raw Milk ◽  
Good Growth ◽  
Lactococcus Lactis Subsp ◽  
Quantitative Expression ◽  
Hard Cheese

ABSTRACT This study evaluated in situ expression of the nisA gene by an indigenous, nisin A–producing (NisA+) Lactococcus lactis subsp. cremoris raw milk genotype, represented by strain M78, in traditional Greek Graviera cheeses under real factory-scale manufacturing and ripening conditions. Cheeses were produced with added a mixed thermophilic and mesophilic commercial starter culture (CSC) or with the CSC plus strain M78 (CSC+M78). Cheeses were sampled after curd cooking (day 0), fermentation of the unsalted molds for 24 h (day 1), brining (day 7), and ripening of the brined molds (14 to 15 kg each) for 30 days in a fully controlled industrial room (16.5°C; 91% relative humidity; day 37). Total RNA was directly extracted from the cheese samples, and the expression of nisA gene was evaluated by real-time reverse transcription PCR (qRT-PCR). Agar overlay and well diffusion bioassays were correspondingly used for in situ detection of the M78 NisA+ colonies in the cheese agar plates and antilisterial activity in whole-cheese slurry samples, respectively. Agar overlay assays showed good growth (>8 log CFU/g of cheese) of the NisA+ strain M78 in coculture with the CSC and vice versa. The nisA expression was detected in CSC+M78 cheese samples only, with its expression levels being the highest (16-fold increase compared with those of the control gene) on day 1, followed by significant reduction on day 7 and almost negligible expression on day 37. Based on the results, certain intrinsic and mainly implicit hurdle factors appeared to reduce growth prevalence rates and decrease nisA gene expression, as well as the nisin A–mediated antilisterial activities of the NisA+ strain M78 postfermentation. To our knowledge, this is the first report on quantitative expression of the nisA gene in a Greek cooked hard cheese during commercial manufacturing and ripening conditions by using a novel, rarely isolated, indigenous NisA+ L. lactis subsp. cremoris genotype as costarter culture.

Quantitative expression of Toll-like receptor-2, -4, and -9 in dendritic cells generated from blasts of patients with acute myeloid leukemia

Transfusion ◽  
2008 ◽  
Vol 48 (5) ◽  
pp. 861-870 ◽  
Author(s):  
Anita Schmitt ◽  
Li Li ◽  
Krzysztof Giannopoulos ◽  
Jochen Greiner ◽  
Peter Reinhardt ◽  
...  
Keyword(s):  
Acute Myeloid Leukemia ◽  
Dendritic Cells ◽  
Myeloid Leukemia ◽  
Toll Like Receptor ◽  
Quantitative Expression ◽  
Toll Like Receptor 2 ◽  
Acute Myeloid

scholarly journals Mechanisms of autoantibody production in autoimmune MRL mice.

1980 ◽  
Vol 152 (5) ◽  
pp. 1302-1310 ◽  
Author(s):  
D S Pisetsky ◽  
G A McCarty ◽  
D V Peters
Keyword(s):  
Antibody Response ◽  
Time Course ◽  
Fundamental Difference ◽  
Autoantibody Production ◽  
Quantitative Expression ◽  
Autoantibody Response ◽  
Time Courses ◽  
Antibody Levels

The quantitative expression of anti-DNA and anti-Sm antibodies has been investigated in autoimmune MRL-lpr/lpr and MRL-+/+ mice. Anti-Sm antibodies were detected in sera from 21/23 lpr/lpr and 10/16 +/+ mice, with individual animals showing striking variation in the time-course and magnitude of this autoantibody response. The peak antibody levels of the responding animals of each substrain did not differ significantly. For anti-DNA antibody, a different pattern of responsiveness was observed. Individual animals of each substrain produced very similar responses in terms of the magnitude and time-course of serum anti-DNA antibody. The differences in the peak levels of the two substrains were highly significant, with lpr/lpr mice demonstrating a much greater anti-DNA antibody response than +/+ mice. In lpr/lpr mice tested for both autoantibody systems, serum anti-DNA and anti-Sm antibodies showed distinct time-courses. These studies indicate that anti-DNA and anti-Sm antibodies are expressed independently in MRL mice, with the expression of anti-DNA, but not anti-Sm antibody markedly influenced by the presence of the 1pr gene. A fundamental difference in the mechanisms involved in the generation of anti-DNA and anti-Sm antibodies is suggested by the quantitative pattern of the two responses.

scholarly journals Quantitative expression and immunogenicity of MAGE-3 and -6 in upper aerodigestive tract cancer

2009 ◽  
Vol 125 (8) ◽  
pp. 1912-1920 ◽  
Author(s):  
Pedro A. Andrade Filho ◽  
Andrés López-Albaitero ◽  
Liqiang Xi ◽  
William Gooding ◽  
Tony Godfrey ◽  
...  
Keyword(s):  
Aerodigestive Tract ◽  
Upper Aerodigestive Tract ◽  
Quantitative Expression ◽  
Tract Cancer ◽  
Upper Aerodigestive Tract Cancer ◽  
Aerodigestive Tract Cancer

scholarly journals Quantitative Expression of the Burial Phenomenon of Deep Seafloor Manganese Nodules

Minerals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 227
Author(s):  
Akira Tsune
Keyword(s):  
Recent Progress ◽  
Mathematical Expression ◽  
Mineral Resources ◽  
Economic Potential ◽  
Visual Data ◽  
Manganese Nodules ◽  
Quantitative Expression ◽  
Size Number ◽  
Polymetallic Nodules ◽  
Geological Origin

Manganese (polymetallic) nodules on the deep seafloor in the open ocean have attracted great interest because of their economic potential. Visual data on nodules found on the deep seafloor such as photographs and videos have increased exponentially with the recent progress of related technologies. These data are expected to reflect useful information for estimating these mineral resources, as well as understanding their geological origin. Although the size, number, and coverage of manganese nodules have been measured in seafloor images, the burial of such nodules has not been sufficiently examined. This paper focuses on mathematical expression of the burial of the manganese nodules and attempts to quantitatively elucidate relations among burial degree and nodule geological parameters. The results, that is, a dataset obtained by calculations of relations among parameters, are also utilized for considerations of quantitative expression of burial. These considerations are expected to contribute to a better understanding of the geological origin of manganese nodules.

scholarly journals The Attempt of Quantitative Expression for Colour Emotion. Influence of Assessment Method.

2000 ◽  
Vol 56 (11) ◽  
pp. 508-517 ◽  
Author(s):  
Taeko Nakamura ◽  
Hiroshi Hoshino ◽  
Tetsuya Sato ◽  
Kanji Kajiwara
Keyword(s):  
Assessment Method ◽  
Quantitative Expression
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