Analysis of Single Cells Using Lab-on-a-Chip Systems

2010 ◽  
Author(s):  
Tobias Preckel
Keyword(s):  
Single Cells ◽  
Lab On A Chip

scholarly journals 3D printed microfluidic lab-on-a-chip device for fiber-based dual beam optical manipulation

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Haoran Wang ◽  
Anton Enders ◽  
John-Alexander Preuss ◽  
Janina Bahnemann ◽  
Alexander Heisterkamp ◽  
...  
Keyword(s):  
Optical Fibers ◽  
Single Cells ◽  
Lab On A Chip ◽  
Cost Effective ◽  
Optical Manipulation ◽  
Hydrodynamic Focusing ◽  
Trapping Region ◽  
Dual Beam ◽  
3D Printed ◽  
On Chip

Abstract3D printing of microfluidic lab-on-a-chip devices enables rapid prototyping of robust and complex structures. In this work, we designed and fabricated a 3D printed lab-on-a-chip device for fiber-based dual beam optical manipulation. The final 3D printed chip offers three key features, such as (1) an optimized fiber channel design for precise alignment of optical fibers, (2) an optically clear window to visualize the trapping region, and (3) a sample channel which facilitates hydrodynamic focusing of samples. A square zig–zag structure incorporated in the sample channel increases the number of particles at the trapping site and focuses the cells and particles during experiments when operating the chip at low Reynolds number. To evaluate the performance of the device for optical manipulation, we implemented on-chip, fiber-based optical trapping of different-sized microscopic particles and performed trap stiffness measurements. In addition, optical stretching of MCF-7 cells was successfully accomplished for the purpose of studying the effects of a cytochalasin metabolite, pyrichalasin H, on cell elasticity. We observed distinct changes in the deformability of single cells treated with pyrichalasin H compared to untreated cells. These results demonstrate that 3D printed microfluidic lab-on-a-chip devices offer a cost-effective and customizable platform for applications in optical manipulation.

Lower Limits of Detection for Single Biological Particles Using Impedance Spectroscopy

2005 ◽  
Author(s):  
Pahnit Seriburi ◽  
Ashutosh Shastry ◽  
Angelique Van’t Wout ◽  
John Mittler ◽  
Shih-Hui Chao ◽  
...  
Keyword(s):  
Theoretical Model ◽  
Impedance Spectroscopy ◽  
Minimally Invasive ◽  
Limit Of Detection ◽  
Single Cells ◽  
Lab On A Chip ◽  
Minimally Invasive Approach ◽  
Invasive Approach ◽  
Membrane Bound ◽  
Lower Limits

Single-cell impedance spectroscopy integrated with lab-on-a-chip systems provides a direct and minimally invasive approach for monitoring and characterizing properties of individual cells in real-time. Here we investigate the theoretical potential and limitations of this technique for analyzing single membrane-bound particles as small as 100 nm in diameter. Our theoretical model suggests a lower limit of detection for single cells on the order of a few microns.

Impedance spectra of patch clamp scenarios for single cells immobilized on a lab-on-a-chip

2013 ◽  
Vol 17 (2) ◽  
pp. 263-274 ◽  
Author(s):  
M. Alberti ◽  
D. Snakenborg ◽  
J. M. Lopacinska ◽  
M. Dufva ◽  
J. P. Kutter
Keyword(s):  
Patch Clamp ◽  
Single Cells ◽  
Lab On A Chip ◽  
Impedance Spectra

scholarly journals Lab-On-A-Chip Device for Yeast Cell Characterization in Low-Conductivity Media Combining Cytometry and Bio-Impedance

Sensors ◽  
2019 ◽  
Vol 19 (15) ◽  
pp. 3366 ◽  
Author(s):  
Julien Claudel ◽  
Arthur Luiz Alves De Araujo ◽  
Mustapha Nadi ◽  
Djilali Kourtiche
Keyword(s):  
Electrical Properties ◽  
Tap Water ◽  
Single Cells ◽  
Short Circuit ◽  
Impedance Measurement ◽  
Lab On A Chip ◽  
Yeast Cells ◽  
Pass Band ◽  
Internal Cell ◽  
Conductivity Medium

This paper proposes a simple approach to optimize the operating frequency band of a lab-on-a-chip based on bio-impedance cytometry for a single cell. It mainly concerns applications in low-conductivity media. Bio-impedance allows for the characterization of low cell concentration or single cells by providing an electrical signature. Thus, it may be necessary to perform impedance measurements up to several tens of megahertz in order to extract the internal cell signature. In the case of single cells, characterization is performed in a very small volume down to 1 pL. At the same time, measured impedances increase from tens of kilo-ohms for physiological liquids up to several mega-ohms for low conductivity media. This is, for example, the case for water analysis. At frequencies above hundreds of kilohertz, parasitic effects, such as coupling capacitances, can prevail over the impedance of the sample and completely short-circuit measurements. To optimize the sensor under these conditions, a complete model of a cytometry device was developed, including parasitic coupling capacitances of the sensor to take into account all the impedances. It appears that it is possible to increase the pass band by optimizing track geometries and placement without changing the sensing area. This assumption was obtained by measuring and comparing electrical properties of yeast cells in a low-conductivity medium (tap water). Decreased coupling capacitance by a factor higher than 10 was obtained compared with a previous non-optimized sensor, which allowed for the impedance measurement of all electrical properties of cells as small as yeast cells in a low-conductivity medium.

Single cells as experimentation units in lab-on-a-chip devices

2010 ◽  
Vol 28 (2) ◽  
pp. 55-62 ◽  
Author(s):  
Séverine Le Gac ◽  
Albert van den Berg
Keyword(s):  
Single Cells ◽  
Lab On A Chip

Single-cell analysis based on lab on a chip fluidic system

2015 ◽  
Vol 7 (20) ◽  
pp. 8524-8533 ◽  
Author(s):  
Alireza Valizadeh ◽  
Ahmad Yari Khosroushahi
Keyword(s):  
Single Cell ◽  
Cell Biology ◽  
Single Cell Analysis ◽  
Single Cells ◽  
Lab On A Chip ◽  
Spatiotemporal Analysis ◽  
Cell Analysis ◽  
System P

The combination of nano/microfabrication-based technologies with cell biology has laid the foundation for facilitating the spatiotemporal analysis of single cells under well-defined physiologically relevant conditions.

scholarly journals Lab-on-a-chip technologies for proteomic analysis from isolated cells

2008 ◽  
Vol 5 (suppl_2) ◽  
Author(s):  
H Sedgwick ◽  
F Caron ◽  
P.B Monaghan ◽  
W Kolch ◽  
J.M Cooper
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescence Microscopy ◽  
Fluorescent Protein ◽  
Single Cells ◽  
Lab On A Chip ◽  
Carcinoma Cells ◽  
Isolated Cells ◽  
Confocal Fluorescence ◽  
On Chip ◽  
Green Fluorescent

Lab-on-a-chip systems offer a versatile environment in which low numbers of cells and molecules can be manipulated, captured, detected and analysed. We describe here a microfluidic device that allows the isolation, electroporation and lysis of single cells. A431 human epithelial carcinoma cells, expressing a green fluorescent protein-labelled actin, were trapped by dielectrophoresis within an integrated lab-on-a-chip device containing saw-tooth microelectrodes. Using these same trapping electrodes, on-chip electroporation was performed, resulting in cell lysis. Protein release was monitored by confocal fluorescence microscopy.

Effects of carbamylcholine on chick embryo aggregate retina cell cultures

1988 ◽  
Vol 46 ◽  
pp. 350-351
Author(s):  
Glenn M. Cohen ◽  
Radharaman Ray
Keyword(s):  
Cell Cultures ◽  
Single Cells ◽  
Retinal Cell ◽  
Cell Aggregates ◽  
High Concentration ◽  
In Ovo ◽  
Sterile Culture ◽  
Retinal Tissue ◽  
Synaptic Junctions ◽  
And Control

Retinal,cell aggregates develop in culture in a pattern similar to the in ovo retina, forming neurites first and then synapses. In the present study, we continuously exposed chick retinal cell aggregates to a high concentration (1 mM) of carbamylcholine (carbachol), an acetylcholine (ACh) analog that resists hydrolysis by acetylcholinesterase (AChE). This situation is similar to organophosphorus anticholinesterase poisoning in which the ACh level is elevated at synaptic junctions due to inhibition of AChE, Our objective was to determine whether continuous carbachol exposure either damaged cholino- ceptive neurites, cell bodies, and synaptic elements of the aggregates or influenced (hastened or retarded) their development.The retinal tissue was isolated aseptically from 11 day embryonic White Leghorn chicks and then enzymatically (trypsin) and mechanically (trituration) dissociated into single cells. After washing the cells by repeated suspension and low (about 200 x G) centrifugation twice, aggregate cell cultures (about l0 cells/culture) were initiated in 1.5 ml medium (BME, GIBCO) in 35 mm sterile culture dishes and maintained as experimental (containing 10-3 M carbachol) and control specimens.

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