Artificial MicroRNA

2011 ◽  
pp. 113-124
Author(s):  
Pranjal Yadava
Keyword(s):  
Artificial Microrna

scholarly journals Effective inhibition of cancer cells by recombinant adenovirus expressing EGFR-targeting artificial microRNA and reversed-caspase-3

PLoS ONE ◽  
2020 ◽  
Vol 15 (8) ◽  
pp. e0237098 ◽  
Author(s):  
Maoxiao Yan ◽  
Jia Chen ◽  
Hua Jiang ◽  
Yuqiong Xie ◽  
Chunchun Li ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Caspase 3 ◽  
Recombinant Adenovirus ◽  
Artificial Microrna ◽  
Effective Inhibition

Artificial MicroRNA and Its Applications

2011 ◽  
pp. 505-521 ◽  
Author(s):  
Pranjal Yadava ◽  
Sunil Kumar Mukherjee
Keyword(s):  
Artificial Microrna

scholarly journals Development of a Dual-Vector System Utilizing MicroRNA Mimics of the Autographa californica miR-1 for an Inducible Knockdown in Insect Cells

2019 ◽  
Vol 20 (3) ◽  
pp. 533
Author(s):  
Krisztina Koczka ◽  
Wolfgang Ernst ◽  
Dieter Palmberger ◽  
Miriam Klausberger ◽  
Lisa Nika ◽  
...  
Keyword(s):  
Fluorescence Intensity ◽  
Insect Cell ◽  
Fluorescent Protein ◽  
Expression System ◽  
Yellow Fluorescent Protein ◽  
Mrna Level ◽  
Vector System ◽  
Sf9 Cells ◽  
Artificial Microrna ◽  
T7 Promoter

The baculovirus-insect cell expression system is a popular tool for the manufacturing of various attractive recombinant products. Over the years, several attempts have been made to engineer and further improve this production platform by targeting host or baculoviral genes by RNA interference. In this study, an inducible knockdown system was established in insect (Sf9) cells by combining an artificial microRNA precursor mimic of baculoviral origin and the bacteriophage T7 transcription machinery. Four structurally different artificial precursor constructs were created and tested in a screening assay. The most efficient artificial microRNA construct resulted in a 69% reduction in the fluorescence intensity of the target enhanced yellow fluorescent protein (eYFP). Next, recombinant baculoviruses were created carrying either the selected artificial precursor mimic under the transcriptional control of the T7 promoter or solely the T7 RNA polymerase under a baculoviral promoter. Upon co-infecting Sf9 cells with these two viruses, the fluorescence intensity of eYFP was suppressed by ~30–40% on the protein level. The reduction in the target mRNA level was demonstrated with real-time quantitative PCR. The presented inducible knockdown system may serve as an important and valuable tool for basic baculovirus-insect cell research and for the improvement of production processes using this platform.

Enzymatic preparation of an artificial microRNA library

2009 ◽  
Vol 390 (3) ◽  
pp. 791-796 ◽  
Author(s):  
Liang Xue ◽  
Qing Yuan ◽  
Ying Yang ◽  
Jiarui Wu
Keyword(s):  
Artificial Microrna ◽  
Enzymatic Preparation

scholarly journals Undesired Small RNAs Originate from an Artificial microRNA Precursor in Transgenic Petunia (Petunia hybrida)

PLoS ONE ◽  
2014 ◽  
Vol 9 (6) ◽  
pp. e98783 ◽  
Author(s):  
Yulong Guo ◽  
Yao Han ◽  
Jing Ma ◽  
Huiping Wang ◽  
Xianchun Sang ◽  
...  
Keyword(s):  
Small Rnas ◽  
Petunia Hybrida ◽  
Artificial Microrna ◽  
Transgenic Petunia

Hepatic Stellate Cell-Specific Gene Silencing Induced by an Artificial MicroRNA for Antifibrosis In Vitro

2009 ◽  
Vol 55 (3) ◽  
pp. 642-653 ◽  
Author(s):  
Ying Chang ◽  
Hua-jun Jiang ◽  
Xue-mei Sun ◽  
Xiao-kun Cai ◽  
Xing-xing He ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Hepatic Stellate Cell ◽  
Stellate Cell ◽  
Specific Gene ◽  
Artificial Microrna
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