Acute RNA Interference for Basic Research and Therapy

2011 ◽  
pp. 1-16
Author(s):  
Federico Calegari
Keyword(s):  
Rna Interference ◽  
Basic Research

scholarly journals RNA interference in biology and disease

Blood ◽  
2005 ◽  
Vol 106 (3) ◽  
pp. 787-794 ◽  
Author(s):  
Carol A. Sledz ◽  
Bryan R. G. Williams
Keyword(s):  
Gene Expression ◽  
Rna Interference ◽  
Large Scale ◽  
Target Gene ◽  
Therapeutic Potential ◽  
Research Effort ◽  
Leukemia Cell ◽  
Basic Research ◽  
Target Gene Expression ◽  
Biologic Response

Abstract RNA interference (RNAi) is a conserved biologic response to double-stranded RNA that results in the sequence-specific silencing of target gene expression. Over the past 5 years, an intensive research effort has facilitated the rapid movement of RNAi from a relatively obscure biologic phenomenon to a valuable tool used to silence target gene expression and perform large-scale functional genomic screens. In fact, recent studies reported in this journal and others have demonstrated success using RNAi to address the role of oncogene expression in leukemia cell lines and to validate the therapeutic potential of RNAi for treating these blood disorders. In order to advance these applications and gain an appreciation for the future of RNAi both in basic research and in the treatment of diseases caused by aberrant gene expression, it is important to have an understanding of the process of RNAi and its limitations.

Gene silencing to induce pathogen-derived resistance in plants.

2021 ◽  
pp. 4-13
Author(s):  
Elena Zuriaga ◽  
Ángela Polo-Oltra ◽  
Maria Luisa Badenes
Keyword(s):  
Rna Interference ◽  
Gene Silencing ◽  
Basic Research ◽  
Rnai Technology ◽  
History Of ◽  
Pathogen Derived Resistance ◽  
Target Effects

Abstract This chapter provides information on the history of the use of pathogen-derived resistance (PDR) in plants and use of PDR for basic research and commercial purposes. Some limitations of the RNA interference (RNAi) technology are presented and various tools that can be used to design RNAi constructs and screen for potential off-target effects are also discussed.

scholarly journals Establishing RNAi for basic research and pest control and identification of the most efficient target genes for pest control: a brief guide

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Sonja Mehlhorn ◽  
Vera S. Hunnekuhl ◽  
Sven Geibel ◽  
Ralf Nauen ◽  
Gregor Bucher
Keyword(s):  
Public Health ◽  
Rna Interference ◽  
Special Reference ◽  
Pest Control ◽  
Gene Function ◽  
Target Genes ◽  
Basic Research ◽  
Insect Species ◽  
Biological Research ◽  
Potential Control

AbstractRNA interference (RNAi) has emerged as a powerful tool for knocking-down gene function in diverse taxa including arthropods for both basic biological research and application in pest control. The conservation of the RNAi mechanism in eukaryotes suggested that it should—in principle—be applicable to most arthropods. However, practical hurdles have been limiting the application in many taxa. For instance, species differ considerably with respect to efficiency of dsRNA uptake from the hemolymph or the gut. Here, we review some of the most frequently encountered technical obstacles when establishing RNAi and suggest a robust procedure for establishing this technique in insect species with special reference to pests. Finally, we present an approach to identify the most effective target genes for the potential control of agricultural and public health pests by RNAi.

A New 1,000 kV Electron Microscope

1967 ◽  
Vol 25 ◽  
pp. 260-261
Author(s):  
M. Nishigaki ◽  
S. Katagiri ◽  
H. Kimura ◽  
B. Tadano
Keyword(s):  
Electron Microscope ◽  
High Voltage ◽  
Basic Research ◽  
Chromatic Aberration ◽  
High Accuracy ◽  
Biological Specimen ◽  
Voltage Electron ◽  
High Voltage Electron Microscope ◽  
High Voltage Electron

The high voltage electron microscope has many advantageous features in comparison with the ordinary electron microscope. They are a higher penetrating efficiency of the electron, low chromatic aberration, high accuracy of the selected area diffraction and so on. Thus, the high voltage electron microscope becomes an indispensable instrument for the metallurgical, polymer and biological specimen studies. The application of the instrument involves today not only basic research but routine survey in the various fields. Particularly for the latter purpose, the performance, maintenance and reliability of the microscope should be same as those of commercial ones. The authors completed a 500 kV electron microscope in 1964 and a 1,000 kV one in 1966 taking these points into consideration. The construction of our 1,000 kV electron microscope is described below.

An emerging technology: Nuclear magnetic resonance microscopy

1988 ◽  
Vol 46 ◽  
pp. 1000-1001
Author(s):  
M.J. Hennessy ◽  
E. Kwok
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Relaxation Times ◽  
Basic Research ◽  
Local Environment ◽  
Magnetic Resonance Images ◽  
Nmr Microscopy ◽  
Mri Scans ◽  
Temperature Relaxation ◽  
Nuclear Magnetic

Much progress in nuclear magnetic resonance microscope has been made in the last few years as a result of improved instrumentation and techniques being made available through basic research in magnetic resonance imaging (MRI) technologies for medicine. Nuclear magnetic resonance (NMR) was first observed in the hydrogen nucleus in water by Bloch, Purcell and Pound over 40 years ago. Today, in medicine, virtually all commercial MRI scans are made of water bound in tissue. This is also true for NMR microscopy, which has focussed mainly on biological applications. The reason water is the favored molecule for NMR is because water is,the most abundant molecule in biology. It is also the most NMR sensitive having the largest nuclear magnetic moment and having reasonable room temperature relaxation times (from 10 ms to 3 sec). The contrast seen in magnetic resonance images is due mostly to distribution of water relaxation times in sample which are extremely sensitive to the local environment.

RNA Interference Technology

2005 ◽  
Author(s):  
Andrew Fire ◽  
Marshall Nirenberg
Keyword(s):  
Rna Interference

Mother-to-child transmission of HIV: developing integration of healthcare programmes with clinical, social and basic research studies

2003 ◽  
Vol 92 (11) ◽  
pp. 1343-1348
Author(s):  
Menu E ◽  
Scarlatti G ◽  
Barré-Sinoussi F ◽  
Gray G ◽  
Bollinger B ◽  
...  
Keyword(s):  
Basic Research ◽  
Mother To Child Transmission ◽  
Child Transmission ◽  
Mother To Child ◽  
Research Studies
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